USP7 (NM_001286458) Human Tagged ORF Clone

CAT#: RC239869

USP7 (myc-DDK-tagged) - Human ubiquitin specific peptidase 7 (herpes virus-associated) (USP7), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: USP7
• Synonyms: HAUSP; TEF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239869 representing NM_001286458
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGATGCCACGCTTTTATCCAGACAGACCACACCAAAAAAGCGTAGGATTCTTTCTCCAGTGCAATG
  CTGAATCTGATTCCACGTCATGGTCTTGCCATGCACAAGCAGTGCTGAAGATAATAAATTACAGAGATGA
  TGAAAAGTCGTTCAGTCGTCGTATTAGTCATTTGTTCTTCCATAAAGAAAATGATTGGGGATTTTCCAAT
  TTTATGGCCTGGAGTGAAGTGACCGATCCTGAGAAAGGATTTATAGATGATGACAAAGTTACCTTTGAAG
  TCTTTGTACAGGCGGATGCTCCCCATGGAGTTGCGTGGGATTCAAAGAAGCACACAGGCTACGTCGGCTT
  AAAGAATCAGGGAGCGACTTGTTACATGAACAGCCTGCTACAGACGTTATTTTTCACGAATCAGCTACGA
  AAGGCTGTGTACATGATGCCAACCGAGGGGGATGATTCGTCTAAAAGCGTCCCTTTAGCATTACAAAGAG
  TGTTCTATGAATTACAGCATAGTGATAAACCTGTAGGAACAAAAAAGTTAACAAAGTCATTTGGGTGGGA
  AACTTTAGATAGCTTCATGCAACATGATGTTCAGGAGCTTTGTCGAGTGTTGCTCGATAATGTGGAAAAT
  AAGATGAAAGGCACCTGTGTAGAGGGCACCATACCCAAATTATTCCGCGGCAAAATGGTGTCCTATATCC
  AGTGTAAAGAAGTAGACTATCGGTCTGATAGAAGAGAAGATTATTATGATATCCAGCTAAGTATCAAAGG
  AAAGAAAAATATATTTGAATCATTTGTGGATTATGTGGCAGTAGAACAGCTCGATGGGGACAATAAATAC
  GACGCTGGGGAACATGGCTTACAGGAAGCAGAGAAAGGTGTGAAATTCCTAACATTGCCACCAGTGTTAC
  ATCTACAACTGATGAGATTTATGTATGACCCTCAGACGGACCAAAATATCAAGATCAATGATAGGTTTGA
  ATTCCCAGAGCAGTTACCACTTGATGAATTTTTGCAAAAAACAGATCCTAAGGACCCTGCAAATTATATT
  CTTCATGCAGTCCTGGTTCATAGTGGAGATAATCATGGTGGACATTATGTGGTTTATCTAAACCCCAAAG
  GGGATGGCAAATGGTGTAAATTTGATGACGACGTGGTGTCAAGGTGTACTAAAGAGGAAGCAATTGAGCA
  CAATTATGGGGGTCACGATGACGACCTGTCTGTTCGACACTGCACTAATGCTTACATGTTAGTCTACATC
  AGGGAATCAAAACTGAGTGAAGTTTTACAGGCGGTCACCGACCATGATATTCCTCAGCAGTTGGTGGAGC
  GATTACAAGAAGAGAAAAGGATCGAGGCTCAGAAGCGGAAGGAGCGGCAGGAAGCCCATCTCTATATGCA
  AGTGCAGATAGTCGCAGAGGACCAGTTTTGTGGCCACCAAGGGAATGACATGTACGATGAAGAAAAAGTG
  AAATACACTGTGTTCAAAGTATTGAAGAACTCCTCGCTTGCTGAGTTTGTTCAGAGCCTCTCTCAGACCA
  TGGGATTTCCACAAGATCAAATTCGATTGTGGCCCATGCAAGCAAGGAGTAATGGAACAAAACGACCAGC
  AATGTTAGATAATGAAGCCGACGGCAATAAAACAATGATTGAGCTCAGTGATAATGAAAACCCTTGGACA
  ATATTCCTGGAAACAGTTGATCCCGAGCTGGCTGCTAGTGGAGCGACCTTACCCAAGTTTGATAAAGATC
  ATGATGTAATGTTATTTTTGAAGATGTATGATCCCAAAACGCGGAGCTTGAATTACTGTGGGCATATCTA
  CACACCAATATCCTGTAAAATACGTGACTTGCTCCCAGTTATGTGTGACAGAGCAGGATTTATTCAAGAT
  ACTAGCCTTATCCTCTATGAGGAAGTTAAACCGAATTTAACAGAGAGAATTCAGGACTATGACGTGTCTC
  TTGATAAAGCCCTTGATGAACTAATGGATGGTGACATCATAGTATTTCAGAAGGATGACCCTGAAAATGA
  TAACAGTGAATTACCCACCGCAAAGGAGTATTTCCGAGATCTCTACCACCGCGTTGATGTCATTTTCTGT
  GATAAAACAATCCCTAATGATCCTGGATTTGTGGTTACGTTATCAAATAGAATGAATTATTTTCAGGTTG
  CAAAGACAGTTGCACAGAGGCTCAACACAGATCCAATGTTGCTGCAGTTTTTCAAGTCTCAAGGTTATAG
  GGATGGCCCAGGTAATCCTCTTAGACATAATTATGAAGGTACTTTAAGAGATCTTCTACAGTTCTTCAAG
  CCTAGACAACCTAAGAAACTTTACTATCAGCAGCTTAAGATGAAAATCACAGACTTTGAGAACAGGCGAA
  GTTTTAAATGTATATGGTTAAACAGCCAATTTAGGGAAGAGGAAATAACACTATATCCAGACAAGCATGG
  GTGTGTCCGGGACCTGTTAGAAGAATGTAAAAAGGCCGTGGAGCTTGGGGAGAAAGCATCAGGGAAACTT
  AGGCTGCTAGAAATTGTAAGCTACAAAATCATTGGTGTTCATCAAGAAGATGAACTATTAGAATGTTTAT
  CTCCTGCAACGAGCCGGACGTTTCGAATAGAGGAAATCCCTTTGGACCAGGTGGACATAGACAAAGAGAA
  TGAGATGCTTGTCACAGTGGCGCATTTCCACAAAGAGGTCTTCGGAACGTTCGGAATCCCGTTTTTGCTG
  AGGATACACCAGGGCGAGCATTTTCGAGAAGTGATGAAGCGAATCCAGAGCCTGCTGGACATCCAGGAGA
  AGGAGTTTGAGAAGTTTAAATTTGCAATTGTAATGATGGGCCGACACCAGTACATAAATGAAGACGAGTA
  TGAAGTAAATTTGAAAGACTTTGAGCCACAGCCCGGTAATATGTCTCATCCTCGGCCTTGGCTAGGGCTC
  GACCACTTCAACAAAGCCCCAAAGAGGAGTCGCTACACTTACCTTGAAAAGGCCATTAAAATCCATAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239869 representing NM_001286458
  Red=Cloning site Green=Tags(s)
  
  MVMPRFYPDRPHQKSVGFFLQCNAESDSTSWSCHAQAVLKIINYRDDEKSFSRRISHLFFHKENDWGFSN
  FMAWSEVTDPEKGFIDDDKVTFEVFVQADAPHGVAWDSKKHTGYVGLKNQGATCYMNSLLQTLFFTNQLR
  KAVYMMPTEGDDSSKSVPLALQRVFYELQHSDKPVGTKKLTKSFGWETLDSFMQHDVQELCRVLLDNVEN
  KMKGTCVEGTIPKLFRGKMVSYIQCKEVDYRSDRREDYYDIQLSIKGKKNIFESFVDYVAVEQLDGDNKY
  DAGEHGLQEAEKGVKFLTLPPVLHLQLMRFMYDPQTDQNIKINDRFEFPEQLPLDEFLQKTDPKDPANYI
  LHAVLVHSGDNHGGHYVVYLNPKGDGKWCKFDDDVVSRCTKEEAIEHNYGGHDDDLSVRHCTNAYMLVYI
  RESKLSEVLQAVTDHDIPQQLVERLQEEKRIEAQKRKERQEAHLYMQVQIVAEDQFCGHQGNDMYDEEKV
  KYTVFKVLKNSSLAEFVQSLSQTMGFPQDQIRLWPMQARSNGTKRPAMLDNEADGNKTMIELSDNENPWT
  IFLETVDPELAASGATLPKFDKDHDVMLFLKMYDPKTRSLNYCGHIYTPISCKIRDLLPVMCDRAGFIQD
  TSLILYEEVKPNLTERIQDYDVSLDKALDELMDGDIIVFQKDDPENDNSELPTAKEYFRDLYHRVDVIFC
  DKTIPNDPGFVVTLSNRMNYFQVAKTVAQRLNTDPMLLQFFKSQGYRDGPGNPLRHNYEGTLRDLLQFFK
  PRQPKKLYYQQLKMKITDFENRRSFKCIWLNSQFREEEITLYPDKHGCVRDLLEECKKAVELGEKASGKL
  RLLEIVSYKIIGVHQEDELLECLSPATSRTFRIEEIPLDQVDIDKENEMLVTVAHFHKEVFGTFGIPFLL
  RIHQGEHFREVMKRIQSLLDIQEKEFEKFKFAIVMMGRHQYINEDEYEVNLKDFEPQPGNMSHPRPWLGL
  DHFNKAPKRSRYTYLEKAIKIHN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286458
• ORF Size: 3009 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001286458.1, NP_001273387.1
• RefSeq Size: 5456
• RefSeq ORF: 3012
• Locus ID: 7874
• Protein Families: Druggable Genome, Protease
• MW: 117.4 kDa
• Gene Summary: The protein encoded by this gene belongs to the peptidase C19 family, which includes ubiquitinyl hydrolases. This protein deubiquitinates target proteins such as p53 (a tumor suppressor protein) and WASH (essential for endosomal protein recycling), and regulates their activities by counteracting the opposing ubiquitin ligase activity of proteins such as HDM2 and TRIM27, involved in the respective process. Mutations in this gene have been implicated in a neurodevelopmental disorder. [provided by RefSeq, Mar 2016]