DDX46 (NM_001300860) Human Tagged ORF Clone

CAT#: RC239901

DDX46 (myc-DDK-tagged) - Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 (DDX46), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DDX46
• Synonyms: Prp5; PRPF5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239901 representing NM_001300860
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTCGGGAGTCACGCCACTATCGAAAACGATCGGCATCCCGGGGTCGCTCTGGAAGTCGGTCTAGAA
  GTCGCTCACCCTCAGACAAAAGAAGTAAACGTGGAGATGACAGACGGTCTAGAAGTAGAGATAGAGATAG
  GAGGAGAGAGAGGTCTCGTAGCAGGGATAAAAGAAGATCTCGGTCAAGGGACAGGAAGCGTCTGAGACGT
  TCCAGAAGTAGAGAGAGAGACAGAAGCCGAGAGCGAAGAAGATCTCGAAGTAGAGACAGGAGACGCTCAA
  GGAGTAGAAGCCGGGGCCGGCGATCCCGATCCTCCAGTCCTGGAAATAAAAGCAAGAAAACTGAGAATAG
  ATCTAGGTCCAAAGAGAAAACTGATGGTGGGGAAAGTTCTAAAGAGAAGAAAAAAGACAAAGATGACAAG
  GAGGATGAAAAAGAAAAAGATGCTGGCAACTTTGACCAGAATAAGCTGGAAGAAGAAATGAGAAAGCGAA
  AAGAAAGAGTAGAAAAATGGCGAGAAGAGCAACGTAAAAAGGCTATGGAAAACATAGGAGAACTGAAAAA
  GGAAATCGAAGAGATGAAACAAGGGAAAAAGTGGAGTTTAGAGGACGATGATGATGACGAAGATGATCCT
  GCAGAAGCTGAAAAGGAGGGAAATGAAATGGAGGGTGAGGAGTTAGATCCATTAGATGCTTACATGGAAG
  AAGTGAAAGAGGAAGTAAAAAAATTTAACATGAGAAGTGTAAAAGGTGGTGGGGGAAATGAAAAGAAGTC
  TGGGCCAACGGTCACAAAAGTTGTCACTGTTGTGACAACCAAAAAAGCAGTTGTGGATTCTGATAAGAAG
  AAAGGTGAGCTGATGGAGAATGACCAGGATGCCATGGAGTATTCTTCAGAGGAGGAAGAAGTTGATCTTC
  AGACAGCCCTTACAGGGTATCAAACAAAACAGCGAAAGCTTCTAGAACCAGTTGATCATGGAAAAATTGA
  GTATGAGCCATTTAGGAAAAACTTCTATGTTGAAGTTCCAGAACTAGCAAAAATGTCTCAAGAAGAGGTA
  AATGTGTTTCGATTGGAAATGGAGGGCATTACAGTTAAAGGAAAAGGTTGCCCCAAACCAATTAAATCCT
  GGGTCCAGTGTGGAATTTCCATGAAGATCTTAAATTCCCTCAAGAAGCATGGCTATGAAAAGCCCACGCC
  CATCCAAACCCAAGCTATTCCTGCTATAATGTCTGGACGAGATTTGATTGGCATTGCCAAAACAGGAAGT
  GGAAAGACCATTGCTTTTCTGTTGCCCATGTTTAGACACATCATGGATCAGAGGTCATTAGAGGAAGGAG
  AGGGGCCAATAGCTGTCATCATGACTCCAACTCGAGAACTGGCTTTACAGATTACTAAAGAGTGTAAGAA
  GTTTTCCAAGACTTTGGGACTTAGAGTGGTCTGTGTTTACGGAGGAACAGGAATCAGTGAGCAGATTGCT
  GAGCTGAAAAGAGGTGCTGAAATTATTGTTTGCACACCTGGTCGAATGATTGACATGTTAGCCGCTAACA
  GTGGTCGGGTCACAAATCTTCGAAGAGTGACATATGTTGTTTTAGATGAAGCAGACAGAATGTTTGACAT
  GGGTTTTGAACCCCAGGTCATGCGCATCGTGGATAATGTTCGTCCTGATCGACAGACGGTTATGTTTTCA
  GCTACTTTCCCCAGAGCTATGGAGGCTTTGGCTCGCAGGATCCTCAGTAAACCTATTGAAGTACAAGTTG
  GAGGCAGGAGTGTGGTTTGCTCAGATGTGGAGCAACAAGTGATTGTGATTGAAGAAGAAAAGAAATTCTT
  GAAGTTACTTGAGCTTCTAGGCCATTATCAAGAGTCAGGATCTGTCATTATATTTGTGGATAAGCAGGAA
  CATGCTGATGGTCTTCTTAAGGATTTAATGAGAGCATCTTATCCTTGCATGTCTCTTCATGGAGGCATTG
  ATCAATATGACAGAGATAGCATCATAAATGACTTTAAGAATGGGACCTGCAAACTTCTTGTGGCTACCTC
  TGTTGCTGCCCGAGGTCTAGATGTGAAACATCTGATTCTTGTAGTAAATTATAGCTGCCCCAACCATTAT
  GAGGATTATGTACACAGAGCAGGGCGGACTGGAAGAGCAGGAAACAAGGGTTATGCTTATACTTTTATCA
  CAGAGGATCAAGCTCGCTATGCTGGTGACATAATTAAAGCTCTTGAATTGTCAGGGACTGCAGTACCTCC
  TGATTTAGAGAAACTGTGGAGTGATTTCAAAGATCAGCAGAAAGCTGAGGGGAAAATAATTAAAAAGAGT
  AGTGGGTTCTCTGGTAAGGGATTCAAGTTTGATGAAACAGAACAAGCTTTGGCTAATGAGAGGAAGAAGT
  TACAAAAAGCAGCTCTTGGTCTACAAGATTCAGATGATGAGGATGCTGCAGTTGATATTGATGAGCAAAT
  TGAAAGCATGTTTAATTCAAAGAAGAGAGTAAAGGATATGGCTGCTCCTGGAACATCAAGTGTTCCTGCT
  CCAACTGCAGGAAATGCTGAGAAATTAGAAATTGCTAAGAGATTGGCTCTTAGAATCAATGCCCAGAAGA
  ATTTGGGCATCGAGTCTCAGGTAGATGTGATGCAGCAGGCCACCAATGCAATTCTTAGGGGTGGCACCAT
  TCTGGCTCCCACTGTTTCTGCAAAAACCATTGCAGAACAACTTGCTGAAAAGATCAATGCCAAGCTCAAT
  TATGTGCCGTTAGAGAAACAAGAAGAAGAGAGACAGGATGGTGGACAGAATGAATCTTTTAAGAGATATG
  AAGAAGAATTAGAGATCAATGACTTCCCACAGACTGCTAGGTGGAAAGTTACCTCTAAGGAAGCTCTGCA
  GAGAATCAGTGAATACTCTGAAGCCGCAATTACAATCAGAGGAACCTACTTCCCTCCTGGCAAAGAACCC
  AAGGAAGGCGAGCGGAAGATTTACTTGGCAATTGAAAGTGCCAATGAACTGGCTGTGCAGAAAGCAAAGG
  CAGAAATCACCAGGCTCATAAAAGAAGAGCTGATCCGGCTGCAAAATTCATACCAACCAACAAATAAAGG
  AAGATACAAAGTCTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239901 representing NM_001300860
  Red=Cloning site Green=Tags(s)
  
  MGRESRHYRKRSASRGRSGSRSRSRSPSDKRSKRGDDRRSRSRDRDRRRERSRSRDKRRSRSRDRKRLRR
  SRSRERDRSRERRRSRSRDRRRSRSRSRGRRSRSSSPGNKSKKTENRSRSKEKTDGGESSKEKKKDKDDK
  EDEKEKDAGNFDQNKLEEEMRKRKERVEKWREEQRKKAMENIGELKKEIEEMKQGKKWSLEDDDDDEDDP
  AEAEKEGNEMEGEELDPLDAYMEEVKEEVKKFNMRSVKGGGGNEKKSGPTVTKVVTVVTTKKAVVDSDKK
  KGELMENDQDAMEYSSEEEEVDLQTALTGYQTKQRKLLEPVDHGKIEYEPFRKNFYVEVPELAKMSQEEV
  NVFRLEMEGITVKGKGCPKPIKSWVQCGISMKILNSLKKHGYEKPTPIQTQAIPAIMSGRDLIGIAKTGS
  GKTIAFLLPMFRHIMDQRSLEEGEGPIAVIMTPTRELALQITKECKKFSKTLGLRVVCVYGGTGISEQIA
  ELKRGAEIIVCTPGRMIDMLAANSGRVTNLRRVTYVVLDEADRMFDMGFEPQVMRIVDNVRPDRQTVMFS
  ATFPRAMEALARRILSKPIEVQVGGRSVVCSDVEQQVIVIEEEKKFLKLLELLGHYQESGSVIIFVDKQE
  HADGLLKDLMRASYPCMSLHGGIDQYDRDSIINDFKNGTCKLLVATSVAARGLDVKHLILVVNYSCPNHY
  EDYVHRAGRTGRAGNKGYAYTFITEDQARYAGDIIKALELSGTAVPPDLEKLWSDFKDQQKAEGKIIKKS
  SGFSGKGFKFDETEQALANERKKLQKAALGLQDSDDEDAAVDIDEQIESMFNSKKRVKDMAAPGTSSVPA
  PTAGNAEKLEIAKRLALRINAQKNLGIESQVDVMQQATNAILRGGTILAPTVSAKTIAEQLAEKINAKLN
  YVPLEKQEEERQDGGQNESFKRYEEELEINDFPQTARWKVTSKEALQRISEYSEAAITIRGTYFPPGKEP
  KEGERKIYLAIESANELAVQKAKAEITRLIKEELIRLQNSYQPTNKGRYKVL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001300860
• ORF Size: 3096 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001300860.1, NP_001287789.1
• RefSeq Size: 5750
• RefSeq ORF: 3099
• Locus ID: 9879
• Protein Pathways: Spliceosome
• MW: 117.9 kDa
• Gene Summary: This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a component of the 17S U2 snRNP complex; it plays an important role in pre-mRNA splicing. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2014]