CACNA2D2 (NM_001291101) Human Tagged ORF Clone

CAT#: RC239941

CACNA2D2 (myc-DDK-tagged) - Human calcium channel, voltage-dependent, alpha 2/delta subunit 2 (CACNA2D2), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CACNA2D2
• Synonyms: CACNA2D
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239941 representing NM_001291101
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCACTGGGCCCGGCGTCTGGAGCAGGAGGTCGACGGCGTGATGCGGATTTTTGGAGGCGTCCAGC
  AGCTCCGTGAGATTTACAAGGACAACCGGAACCTGTTCGAGGTACAGGAGAATGAGCCTCAGAAGTTGGT
  GGAGAAGGTGGCAGGGGACATTGAGAGCCTTCTGGACAGGAAGGTGCAGGCCCTGAAGAGACTGGCTGAT
  GCTGCAGAGAACTTCCAGAAAGCACACCGCTGGCAGGACAACATCAAGGAGGAAGACATCGTGTACTATG
  ACGCCAAGGCTGACGCTGAGCTGGACGACCCTGAGAGTGAGGATGTGGAAAGGGGGTCTAAGGCCAGCAC
  CCTAAGGCTGGACTTCATCGAGGACCCAAACTTCAAGAACAAGGTCAACTATTCATACGCGGCTGTACAG
  ATCCCTACGGACATCTACAAAGGCTCCACTGTCATCCTCAATGAGCTCAACTGGACAGAGGCCCTGGAGA
  ATGTGTTCATGGAAAACCGCAGACAAGACCCCACACTGCTGTGGCAGGTCTTCGGCAGCGCCACAGGAGT
  CACTCGCTACTACCCGGCCACCCCGTGGCGAGCCCCCAAGAAGATCGACCTGTACGATGTCCGAAGGAGA
  CCCTGGTATATCCAGGGGGCCTCGTCACCCAAAGACATGGTCATCATCGTGGATGTGAGTGGCAGTGTGA
  GCGGCCTGACCCTGAAGCTGATGAAGACATCTGTCTGCGAGATGCTGGACACGCTGTCTGATGATGACTA
  TGTGAATGTGGCCTCGTTCAACGAGAAGGCACAGCCTGTGTCATGCTTCACACACCTGGTGCAGGCCAAT
  GTGCGCAACAAGAAGGTGTTCAAGGAAGCTGTGCAGGGCATGGTGGCCAAGGGCACCACAGGCTACAAGG
  CCGGCTTTGAGTATGCCTTTGACCAGCTGCAGAACTCCAACATCACTCGGGCCAACTGCAACAAGATGAT
  CATGATGTTCACGGATGGTGGTGAGGACCGCGTGCAGGACGTCTTTGAGAAGTACAATTGGCCAAACCGG
  ACGGTGCGCGTGTTTACTTTCTCCGTGGGGCAGCATAACTATGACGTCACACCGCTGCAGTGGATGGCCT
  GTGCCAACAAAGGCTACTATTTTGAGATCCCTTCCATCGGAGCCATCCGCATCAACACACAGGAATATCT
  AGATGTGTTGGGCAGGCCCATGGTGCTGGCAGGCAAGGAGGCCAAGCAGGTGCAGTGGACCAACGTGTAT
  GAGGATGCACTGGGACTGGGGTTGGTGGTAACAGGGACCCTCCCTGTTTTCAACCTGACACAGGATGGCC
  CTGGGGAAAAGAAGAACCAGCTGATCCTGGGCGTGATGGGCATTGACGTGGCTCTGAATGACATCAAGAG
  GCTGACCCCCAACTACACGCTTGGAGCCAACGGCTATGTGTTTGCCATTGACCTGAACGGCTACGTGTTG
  CTGCACCCCAATCTCAAGCCCCAGACCACCAACTTCCGGGAGCCTGTGACTCTGGACTTCCTGGATGCGG
  AGCTAGAGGATGAGAACAAGGAAGAGATCCGTCGGAGCATGATTGATGGCAACAAGGGCCACAAGCAGAT
  CAGAACGTTGGTCAAGTCCCTGGATGAGAGGTACATAGATGAGGTGACACGGAACTACACCTGGGTGCCT
  ATAAGGAGCACTAACTACAGCCTGGGGCTGGTGCTCCCACCCTACAGCACCTTCTACCTCCAAGCCAATC
  TCAGTGACCAGATCCTGCAGGTCAAGTATTTTGAGTTCCTGCTCCCCAGCAGCTTTGAGTCTGAAGGACA
  CGTTTTCATTGCTCCCAGAGAGTACTGCAAGGACCTGAATGCCTCAGACAACAACACCGAGTTCCTGAAA
  AACTTTATTGAGCTCATGGAGAAAGTGACTCCAGACTCCAAGCAGTGCAACAACTTCCTTCTGCACAACC
  TGATCTTGGACACGGGCATCACGCAGCAGCTGGTAGAGCGTGTGTGGAGGGACCAGGATCTCAACACGTA
  CAGCCTACTGGCCGTGTTCGCTGCCACAGACGGTGGCATCACCCGAGTCTTCCCCAACAAGGCAGCTGAG
  GACTGGACAGAGAACCCTGAGCCCTTCAATGCCAGCTTCTACCGCCGCAGCCTGGATAACCACGGTTATG
  TCTTCAAGCCCCCACACCAGGATGCCCTGTTAAGGCCGCTGGAGCTGGAGAATGACACTGTGGGCATCCT
  CGTCAGCACAGCTGTGGAGCTCAGCCTAGGCAGGCGCACACTGAGGCCAGCAGTGGTGGGCGTCAAGCTG
  GACCTAGAGGCTTGGGCTGAGAAGTTCAAGGTGCTAGCCAGCAACCGTACCCACCAAGACCAGCCTCAGA
  AGTGCGGCCCCAACAGCCACTGTGAGATGGACTGCGAGGTTAACAATGAGGACTTACTCTGTGTCCTCAT
  TGATGATGGAGGATTCCTGGTGCTGTCAAACCAGAACCATCAGTGGGACCAGGTGGGCAGGTTCTTCAGT
  GAGGTGGATGCCAACCTGATGCTGGCACTCTACAATAACTCCTTCTACACCCGCAAGGAGTCCTATGACT
  ATCAGGCAGCCTGTGCCCCTCAGCCCCCTGGCAACCTGGGTGCTGCACCCCGGGGTGTCTTTGTGCCCAC
  CGTTGCAGATTTCCTTAACCTGGCCTGGTGGACCTCTGCTGCCGCCTGGTCCCTGTTCCAGCAGCTTCTC
  TACGGCCTCATCTACCACAGCTGGTTCCAAGCAGACCCCGCGGAGGCCGAGGGGAGCCCCGAGACGCGCG
  AGAGCAGCTGCGTCATGAAACAGACCCAGTACTACTTCGGCTCGGTAAACGCCTCCTACAACGCCATCAT
  CGACTGCGGAAACTGCTCCAGGCTGTTCCACGCGCAGAGACTGACCAACACCAATCTTCTCTTTGTGGTG
  GCCGAGAAGCCGCTGTGCAGCCAGTGCGAGGCTGGCCGGCTGCTGCAGAAGGAGACGCACTGCCCAGCGG
  ACGGCCCGGAGCAGTGTGAGCTAGTGCAGAGACCGCGATACCGGAGAGGCCCGCACATCTGCTTCGACTA
  CAACGCGACAGAAGATACCTCAGACTGTGGCCGCGGGGCCTCCTTCCCGCCGTCGCTGGGCGTCCTGGTC
  TCCCTGCAACTGCTGCTCCTCCTGGGCCTGCCGCCCCGGCCGCAGCCTCAAGTCCTCGTCCACGCCTCTC
  GCCGCCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239941 representing NM_001291101
  Red=Cloning site Green=Tags(s)
  
  MQHWARRLEQEVDGVMRIFGGVQQLREIYKDNRNLFEVQENEPQKLVEKVAGDIESLLDRKVQALKRLAD
  AAENFQKAHRWQDNIKEEDIVYYDAKADAELDDPESEDVERGSKASTLRLDFIEDPNFKNKVNYSYAAVQ
  IPTDIYKGSTVILNELNWTEALENVFMENRRQDPTLLWQVFGSATGVTRYYPATPWRAPKKIDLYDVRRR
  PWYIQGASSPKDMVIIVDVSGSVSGLTLKLMKTSVCEMLDTLSDDDYVNVASFNEKAQPVSCFTHLVQAN
  VRNKKVFKEAVQGMVAKGTTGYKAGFEYAFDQLQNSNITRANCNKMIMMFTDGGEDRVQDVFEKYNWPNR
  TVRVFTFSVGQHNYDVTPLQWMACANKGYYFEIPSIGAIRINTQEYLDVLGRPMVLAGKEAKQVQWTNVY
  EDALGLGLVVTGTLPVFNLTQDGPGEKKNQLILGVMGIDVALNDIKRLTPNYTLGANGYVFAIDLNGYVL
  LHPNLKPQTTNFREPVTLDFLDAELEDENKEEIRRSMIDGNKGHKQIRTLVKSLDERYIDEVTRNYTWVP
  IRSTNYSLGLVLPPYSTFYLQANLSDQILQVKYFEFLLPSSFESEGHVFIAPREYCKDLNASDNNTEFLK
  NFIELMEKVTPDSKQCNNFLLHNLILDTGITQQLVERVWRDQDLNTYSLLAVFAATDGGITRVFPNKAAE
  DWTENPEPFNASFYRRSLDNHGYVFKPPHQDALLRPLELENDTVGILVSTAVELSLGRRTLRPAVVGVKL
  DLEAWAEKFKVLASNRTHQDQPQKCGPNSHCEMDCEVNNEDLLCVLIDDGGFLVLSNQNHQWDQVGRFFS
  EVDANLMLALYNNSFYTRKESYDYQAACAPQPPGNLGAAPRGVFVPTVADFLNLAWWTSAAAWSLFQQLL
  YGLIYHSWFQADPAEAEGSPETRESSCVMKQTQYYFGSVNASYNAIIDCGNCSRLFHAQRLTNTNLLFVV
  AEKPLCSQCEAGRLLQKETHCPADGPEQCELVQRPRYRRGPHICFDYNATEDTSDCGRGASFPPSLGVLV
  SLQLLLLLGLPPRPQPQVLVHASRRL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001291101
• ORF Size: 3228 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001291101.1, NP_001278030.1
• RefSeq Size: 5461
• RefSeq ORF: 3231
• Locus ID: 9254
• Protein Families: Druggable Genome, Ion Channels: Other
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Cardiac muscle contraction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway
• MW: 122.6 kDa
• Gene Summary: Calcium channels mediate the entry of calcium ions into the cell upon membrane polarization. This gene encodes the alpha-2/delta subunit of the voltage-dependent calcium channel complex. The complex consists of the main channel-forming subunit alpha-1, and auxiliary subunits alpha-2/delta, beta, and gamma. The auxiliary subunits function in the assembly and membrane localization of the complex, and modulate calcium currents and channel activation/inactivation kinetics. The subunit encoded by this gene undergoes post-translational cleavage to yield the extracellular alpha2 peptide and a membrane-anchored delta polypeptide. This subunit is a receptor for the antiepileptic drug, gabapentin. Mutations in this gene are associated with early infantile epileptic encephalopathy. Single nucleotide polymorphisms in this gene are correlated with increased sensitivity to opioid drugs. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2014]