UPF1 (NM_001297549) Human Tagged ORF Clone
CAT#: RC239991
- TrueORF®
UPF1 (myc-DDK-tagged) - Human UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), transcript variant 1
"NM_001297549" in other vectors (1)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | UPF1 |
Synonyms | HUPF1; NORF1; pNORF1; RENT1; smg-2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC239991 representing NM_001297549
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGTGGAGGCGTACGGGCCCAGCTCGCAGACTCTCACTTTCCTGGACACGGAGGAGGCCGAGCTGC TTGGCGCCGACACACAGGGCTCCGAGTTCGAGTTCACCGACTTTACTCTTCCTAGCCAGACGCAGACGCC CCCCGGCGGCCCCGGCGGCCCGGGCGGTGGCGGCGCGGGAGGCCCGGGCGGCGCGGGCGCGGGCGCTGCG GCGGGACAGCTCGACGCGCAGGTTGGGCCCGAAGGCATCCTGCAGAACGGGGCTGTGGACGACAGTGTAG CCAAGACCAGCCAGTTGTTGGCTGAGTTGAACTTCGAGGAAGATGAAGAAGACACCTATTACACGAAGGA CCTCCCCATACACGCCTGCAGTTACTGTGGAATACACGATCCTGCCTGCGTGGTTTACTGTAATACCAGC AAGAAGTGGTTCTGCAACGGACGTGGAAATACTTCTGGCAGCCACATTGTAAATCACCTTGTGAGGGCAA AATGCAAAGAGGTGACCCTGCACAAGGACGGGCCCCTGGGGGAGACAGTCCTGGAGTGCTACAACTGCGG CTGTCGCAACGTCTTCCTCCTCGGCTTCATCCCGGCCAAAGCTGACTCAGTGGTGGTGCTGCTGTGCAGG CAGCCCTGTGCCAGCCAGAGCAGCCTCAAGGACATCAACTGGGACAGCTCGCAGTGGCAGCCGCTGATCC AGGACCGCTGCTTCCTGTCCTGGCTGGTCAAGATCCCCTCCGAGCAGGAGCAGCTGCGGGCACGCCAGAT CACGGCACAGCAGATCAACAAGCTGGAGGAGCTGTGGAAGGAAAACCCTTCTGCCACGCTGGAGGACCTG GAGAAGCCGGGGGTGGACGAGGAGCCGCAGCATGTCCTCCTGCGGTACGAGGACGCCTACCAGTACCAGA ACATATTCGGGCCCCTGGTCAAGCTGGAGGCCGACTACGACAAGAAGCTGAAGGAGTCCCAGACTCAAGA TAACATCACTGTCAGGTGGGACCTGGGCCTTAACAAGAAGAGAATCGCCTACTTCACTTTGCCCAAGACT GACTCTGGTAATGAGGATTTAGTCATAATTTGGTTAAGAGACATGCGGCTCATGCAGGGGGATGAGATAT GCCTGCGGTACAAAGGGGACCTTGCGCCCCTGTGGAAAGGGATCGGCCACGTCATCAAGGTCCCTGATAA TTATGGCGATGAGATCGCCATTGAGCTGCGGAGCAGCGTGGGTGCACCTGTGGAGGTGACTCACAACTTC CAGGTGGATTTTGTGTGGAAGTCGACCTCCTTTGACAGGATGCAGAGCGCATTGAAAACGTTTGCCGTGG ATGAGACCTCGGTGTCTGGCTACATCTACCACAAGCTGTTGGGCCACGAGGTGGAGGACGTAATCATCAA GTGCCAGCTGCCCAAGCGCTTCACGGCGCAGGGCCTCCCCGACCTCAACCACTCCCAGGTTTATGCCGTG AAGACTGTGCTGCAAAGACCACTGAGCCTGATCCAGGGCCCGCCAGGCACGGGGAAGACGGTGACGTCGG CCACCATCGTCTACCACCTGGCCCGGCAAGGCAACGGGCCGGTGCTGGTGTGTGCTCCGAGCAACATCGC CGTGGACCAGCTAACGGAGAAGATCCACCAGACGGGGCTAAAGGTCGTGCGCCTCTGCGCCAAGAGCCGT GAGGCCATCGACTCCCCGGTGTCTTTTCTGGCCCTGCACAACCAGATCAGGAACATGGACAGCATGCCTG AGCTGCAGAAGCTGCAGCAGCTGAAAGACGAGACTGGGGAGCTGTCGTCTGCCGACGAGAAGCGGTACCG GGCCTTGAAGCGCACCGCAGAGAGAGAGCTGCTGATGAACGCAGATGTCATCTGCTGCACATGTGTGGGC GCCGGTGACCCGAGGCTGGCCAAGATGCAGTTCCGCTCCATTTTAATCGACGAAAGCACCCAGGCCACCG AGCCGGAGTGCATGGTTCCCGTGGTCCTCGGGGCCAAGCAGCTGATCCTTGTAGGCGACCACTGCCAGCT GGGCCCAGTGGTGATGTGCAAGAAGGCGGCCAAGGCCGGGCTGTCACAGTCGCTCTTCGAGCGCCTGGTG GTGCTGGGCATCCGGCCCATCCGCCTGCAGGTCCAGTACCGGATGCACCCTGCACTCAGCGCCTTCCCAT CCAACATCTTCTACGAGGGCTCCCTCCAGAATGGTGTCACTGCAGCGGATCGTGTGAAGAAGGGATTTGA CTTCCAGTGGCCCCAACCCGATAAACCGATGTTCTTCTACGTGACCCAGGGCCAAGAGGAGATTGCCAGC TCGGGCACCTCCTACCTGAACAGGACCGAGGCTGCGAACGTGGAGAAGATCACCACGAAGTTGCTGAAGG CAGGCGCCAAGCCGGACCAGATTGGCATCATCACGCCCTACGAGGGCCAGCGCTCCTACCTGGTGCAGTA CATGCAGTTCAGCGGCTCCCTGCACACCAAGCTCTACCAGGAGGTGGAGATCGCCAGTGTGGACGCCTTT CAGGGACGCGAGAAGGACTTCATCATCCTGTCCTGTGTGCGGGCCAACGAGCACCAAGGCATTGGCTTTT TAAATGACCCCAGGCGTCTGAACGTGGCCCTGACCAGAGCAAGGTATGGCGTCATCATTGTGGGCAACCC GAAGGCACTATCAAAGCAGCCGCTCTGGAACCACCTGCTGAACTACTATAAGGAGCAGAAGGTGCTGGTG GAGGGGCCGCTCAACAACCTGCGTGAGAGCCTCATGCAGTTCAGCAAGCCACGGAAGCTGGTCAACACTA TCAACCCGGGAGCCCGCTTCATGACCACAGCCATGTATGATGCCCGGGAGGCCATCATCCCAGGCTCCGT CTATGATCGGAGCAGCCAGGGCCGGCCTTCCAGCATGTACTTCCAGACCCATGACCAGATTGGCATGATC AGTGCCGGCCCTAGCCACGTGGCTGCCATGAACATTCCCATCCCCTTCAACCTGGTCATGCCACCCATGC CACCGCCTGGCTATTTTGGACAAGCCAACGGGCCTGCTGCAGGGCGAGGCACCCCGAAAGGCAAGACTGG TCGTGGGGGACGCCAGAAGAACCGCTTTGGGCTTCCTGGACCCAGCCAGACTAACCTCCCCAACAGCCAA GCCAGCCAGGATGTGGCGTCACAGCCCTTCTCTCAGGGCGCCCTGACGCAGGGCTACATCTCCATGAGCC AGCCTTCCCAGATGAGCCAGCCCGGCCTCTCCCAGCCGGAGCTGTCCCAGGACAGTTACCTTGGTGACGA GTTTAAATCACAAATCGACGTGGCGCTCTCACAGGACTCCACGTACCAGGGAGAGCGGGCTTACCAGCAT GGCGGGGTGACGGGGCTGTCCCAGTAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC239991 representing NM_001297549
Red=Cloning site Green=Tags(s) MSVEAYGPSSQTLTFLDTEEAELLGADTQGSEFEFTDFTLPSQTQTPPGGPGGPGGGGAGGPGGAGAGAA AGQLDAQVGPEGILQNGAVDDSVAKTSQLLAELNFEEDEEDTYYTKDLPIHACSYCGIHDPACVVYCNTS KKWFCNGRGNTSGSHIVNHLVRAKCKEVTLHKDGPLGETVLECYNCGCRNVFLLGFIPAKADSVVVLLCR QPCASQSSLKDINWDSSQWQPLIQDRCFLSWLVKIPSEQEQLRARQITAQQINKLEELWKENPSATLEDL EKPGVDEEPQHVLLRYEDAYQYQNIFGPLVKLEADYDKKLKESQTQDNITVRWDLGLNKKRIAYFTLPKT DSGNEDLVIIWLRDMRLMQGDEICLRYKGDLAPLWKGIGHVIKVPDNYGDEIAIELRSSVGAPVEVTHNF QVDFVWKSTSFDRMQSALKTFAVDETSVSGYIYHKLLGHEVEDVIIKCQLPKRFTAQGLPDLNHSQVYAV KTVLQRPLSLIQGPPGTGKTVTSATIVYHLARQGNGPVLVCAPSNIAVDQLTEKIHQTGLKVVRLCAKSR EAIDSPVSFLALHNQIRNMDSMPELQKLQQLKDETGELSSADEKRYRALKRTAERELLMNADVICCTCVG AGDPRLAKMQFRSILIDESTQATEPECMVPVVLGAKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLV VLGIRPIRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRVKKGFDFQWPQPDKPMFFYVTQGQEEIAS SGTSYLNRTEAANVEKITTKLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAF QGREKDFIILSCVRANEHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALSKQPLWNHLLNYYKEQKVLV EGPLNNLRESLMQFSKPRKLVNTINPGARFMTTAMYDAREAIIPGSVYDRSSQGRPSSMYFQTHDQIGMI SAGPSHVAAMNIPIPFNLVMPPMPPPGYFGQANGPAAGRGTPKGKTGRGGRQKNRFGLPGPSQTNLPNSQ ASQDVASQPFSQGALTQGYISMSQPSQMSQPGLSQPELSQDSYLGDEFKSQIDVALSQDSTYQGERAYQH GGVTGLSQY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001297549 |
ORF Size | 3387 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001297549.1, NP_001284478.1 |
RefSeq Size | 5395 bp |
RefSeq ORF | 3390 bp |
Locus ID | 5976 |
Cytogenetics | 19p13.11 |
Protein Families | Druggable Genome |
MW | 124.8 kDa |
Gene Summary | 'This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC337885 | UPF1 (untagged) - Human UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), transcript variant 1 |
USD 1,140.00 |
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