ASTN1 (NM_001286164) Human Tagged ORF Clone

CAT#: RC240059

ASTN1 (myc-DDK-tagged) - Human astrotactin 1 (ASTN1), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ASTN1
• Synonyms: ASTN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC240059 representing NM_001286164
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTTAGCCGGGCTCTGCGCCCTGCTCGCCTGCTGCTGGGGGCCGGCGGCGGTGCTGGCCACGGCCG
  CCGGCGACGTGGATCCATCCAAGGAGCTGGAGTGCAAGCTCAAAAGCATCACGGTGTCGGCACTGCCCTT
  CCTGCGCGAGAACGACCTGAGCATCATGCACAGCCCCTCGGCCTCGGAGCCCAAGCTCCTCTTCTCGGTG
  CGCAACGACTTCCCGGGAGAAATGGTCGTGGTGGACGACCTGGAGAACACGGAGCTGCCCTACTTCGTGC
  TGGAGATCTCAGGGAACACAGAGGATATCCCTTTGGTGCGCTGGAGGCAGCAGTGGCTGGAGAATGGCAC
  TTTGCTTTTTCACATTCATCACCAAGATGGTGCCCCAAGCCTTCCTGGACAAGACCCCACTGAAGAACCC
  CAACATGAGTCGGCAGAAGAGGAGCTGAGGATCCTCCACATCTCAGTCATGGGTGGCATGATCGCTCTGC
  TGCTGTCCATCTTGTGCCTGGTGATGATCCTGTATACTCGCCGGCGCTGGTGCAAACGCCGCCGGGTCCC
  GCAGCCCCAGAAGAGTGCCAGTGCTGAGGCAGCCAATGAGATTCACTACATTCCTTCTGTGCTGATCGGC
  GGGCACGGACGGGAGAGCCTGCGCAATGCCCGCGTGCAGGGCCACAACTCCAGTGGCACCCTGAGCATCC
  GGGAGACACCTATCCTGGACGGCTATGAGTATGACATCACTGATCTGCGCCACCATCTGCAGAGGGAGTG
  CATGAACGGAGGGGAGGACTTTGCCAGCCAGGTCACGCGCACCCTCGACTCCCTGCAGGGCTGCAATGAA
  AAGTCGGGGATGGACCTCACACCAGGAAGTGACAATGCCAAGCTGTCACTGATGAACAAGTATAAAGATA
  ATATTATAGCCACTAGCCCTGTGGACTCCAACCACCAGCAAGCCACCCTTCTCTCTCACACCTCCAGCAG
  CCAGAGAAAGCGGATCAACAACAAAGCAAGAGCTGGTTCCGCCTTCTTGAACCCTGAAGGGGATTCTGGC
  ACAGAGGCAGAAAACGACCCCCAGCTGACCTTTTACACGGATCCTTCAAGGAGCAGGAGGCGTAGTAGAG
  TGGGTTCTCCCCGAAGTCCTGTGAATAAGACCACCTTGACCCTGATCAGCATCACCAGCTGTGTGATTGG
  CCTCGTGTGCTCCTCTCACGTCAACTGCCCTCTCGTTGTCAAGATCACCCTGCATGTCCCTGAGCACCTG
  ATTGCTGATGGGAGCCGCTTCATCTTGCTGGAGGGGAGCCAGCTGGATGCCAGTGACTGGCTGAACCCTG
  CCCAAGTGGTTCTCTTCTCTCAGCAGAACTCCAGCGGACCCTGGGCCATGGACCTCTGTGCCCGGCGGCT
  CCTGGACCCCTGTGAACACCAATGTGACCCCGAAACTGGGGAATGCCTCTGCTATGAAGGCTACATGAAG
  GATCCAGTACATAAGCACCTTTGCATTCGGAACGAATGGGGGACAAACCAGGGGCCATGGCCTTACACAA
  TATTTCAGCGAGGCTTTGACCTGGTTTTGGGAGAGCAGCCCTCTGATAAAATATTTAGATTCACCTACAC
  TCTTGGGGAGGGCATGTGGTTGCCCCTCAGCAAGAGCTTTGTGATTCCACCAGCCGAACTGGCCATCAAT
  CCATCAGCAAAGTGCAAGACGGACATGACTGTGATGGAGGATGCTGTGGAGGTCAGAGAGGAGCTGATGA
  CTTCATCCTCCTTCGACAGCCTGGAGGTTCTCTTAGATTCCTTTGGGCCGGTGCGCGACTGCAGCAAAGA
  TAACGGGGGCTGCAGTAAGAATTTCCGCTGTATTTCAGATCGCAAGCTGGACTCCACTGGTTGCGTGTGC
  CCATCTGGACTCAGTCCCATGAAGGACAGCTCTGGCTGCTATGACCGCCACATCGGGGTGGACTGTTCCG
  ACGGCTTCAACGGCGGCTGTGAGCAGCTGTGCCTCCAGCAGATGGCGCCCTTCCCGGACGACCCCACCTT
  GTATAACATCCTCATGTTCTGTGGGTGCATCGAGGACTACAAGCTTGGTGTGGATGGACGCTCTTGCCAA
  CTCATCACGGAGACCTGTCCAGAGGGAAGTGACTGTGGGGAAAGCAGGGAGCTTCCCATGAACCAGACCC
  TCTTTGGGGAGATGTTCTTTGGTTACAACAACCATTCCAAGGAAGTGGCTGCCGGACAGGTGCTGAAAGG
  AACATTCAGGCAAAACAACTTTGCTCGTGGTTTAGACCAGCAACTGCCAGATGGTCTTGTGGTGGCCACT
  GTGCCCCTGGAGAATCAATGCCTAGAGGAGATCTCGGAGCCCACCCCTGACCCTGACTTCCTGACTGGGA
  TGGTGAACTTCAGTGAAGTGTCTGGGTACCCTGTGCTGCAGCACTGGAAGGTCCGGTCTGTGATGTACCA
  CATCAAACTCAACCAAGTGGCCATCTCTCAGGCCCTCAGCAATGCTCTCCACTCGCTGGATGGGGCTACA
  TCTCGTGCAGATTTTGTGGCGCTGTTGGACCAGTTCGGCAACCATTACATCCAGGAAGCTATCTACGGCT
  TTGAGGAGTCCTGTTCTATCTGGTACCCAAACAAGCAGGTCCAGCGGCGACTCTGGCTGGAGTATGAAGA
  CATCAGTAAAGGCAACTCCCCATCAGATGAGTCTGAGGAGCGGGAAAGAGACCCCAAGGTGCTGACATTC
  CCAGAATACATCACCAGCTTGTCAGACTCCGGCACCAAGCACATGGCGGCTGGAGTCCGCATGGAGTGCC
  ACAGCAAGGGACGATGCCCCTCGTCCTGCCCCCTGTGTCATGTGACATCCAGCCCTGACACCCCTGCTGA
  GCCGGTTCTGCTGGAGGTGACCAAAGCAGCCCCCATCTATGAACTAGTGACCAACAACCAGACCCAGAGG
  CTCTTGCAGGAGGCTACCATGAGCTCTCTCTGGTGCTCAGGGACTGGAGATGTCATCGAGGACTGGTGTC
  GATGTGACTCCACTGCTTTTGGAGCTGATGGACTCCCCACCTGTGCGCCTCTCCCACAGCCTGTGCTGAG
  ACTCTCCACGGTTCACGAGCCCAGCAGCACTCTTGTGGTCCTGGAGTGGGAACACTCAGAGCCACCAATC
  GGGGTGCAGATTGTAGATTACCTCCTCCGTCAAGAGAAAGTCACTGACAGGATGGACCACTCCAAAGTGG
  AGACAGAAACAGTGCTGAGCTTTGTGGACGACATCATCTCTGGAGCAAAGTCTCCTTGTGCAATGCCATC
  TCAGGTGCCGGACAAGCAGCTCACCACCATCTCTCTGATCATACGATGCCTGGAACCTGACACCATTTAC
  ATGTTCACGCTGTGGGGAGTGGACAACACAGGACGGCGCTCCAGGCCAAGCGACGTGATCGTGAAGACCC
  CATGCCCCGTGGTGGATGATGTCAAGGCTCAAGAAATAGCAGACAAGATCTACAATCTCTTCAATGGCTA
  CACTAGTGGGAAGGAGCAGCAGACCGCCTACAACACCCTCCTGGATCTGGGTTCCCCCACCTTACACCGG
  GTCCTCTACCACTATAACCAGCACTATGAGAGTTTTGGGGAATTCACCTGGCGATGTGAGGATGAGTTAG
  GTCCCAGGCTTCCAGCGTGTTGGGACAGTCAGAGGAGTTGTAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC240059 representing NM_001286164
  Red=Cloning site Green=Tags(s)
  
  MALAGLCALLACCWGPAAVLATAAGDVDPSKELECKLKSITVSALPFLRENDLSIMHSPSASEPKLLFSV
  RNDFPGEMVVVDDLENTELPYFVLEISGNTEDIPLVRWRQQWLENGTLLFHIHHQDGAPSLPGQDPTEEP
  QHESAEEELRILHISVMGGMIALLLSILCLVMILYTRRRWCKRRRVPQPQKSASAEAANEIHYIPSVLIG
  GHGRESLRNARVQGHNSSGTLSIRETPILDGYEYDITDLRHHLQRECMNGGEDFASQVTRTLDSLQGCNE
  KSGMDLTPGSDNAKLSLMNKYKDNIIATSPVDSNHQQATLLSHTSSSQRKRINNKARAGSAFLNPEGDSG
  TEAENDPQLTFYTDPSRSRRRSRVGSPRSPVNKTTLTLISITSCVIGLVCSSHVNCPLVVKITLHVPEHL
  IADGSRFILLEGSQLDASDWLNPAQVVLFSQQNSSGPWAMDLCARRLLDPCEHQCDPETGECLCYEGYMK
  DPVHKHLCIRNEWGTNQGPWPYTIFQRGFDLVLGEQPSDKIFRFTYTLGEGMWLPLSKSFVIPPAELAIN
  PSAKCKTDMTVMEDAVEVREELMTSSSFDSLEVLLDSFGPVRDCSKDNGGCSKNFRCISDRKLDSTGCVC
  PSGLSPMKDSSGCYDRHIGVDCSDGFNGGCEQLCLQQMAPFPDDPTLYNILMFCGCIEDYKLGVDGRSCQ
  LITETCPEGSDCGESRELPMNQTLFGEMFFGYNNHSKEVAAGQVLKGTFRQNNFARGLDQQLPDGLVVAT
  VPLENQCLEEISEPTPDPDFLTGMVNFSEVSGYPVLQHWKVRSVMYHIKLNQVAISQALSNALHSLDGAT
  SRADFVALLDQFGNHYIQEAIYGFEESCSIWYPNKQVQRRLWLEYEDISKGNSPSDESEERERDPKVLTF
  PEYITSLSDSGTKHMAAGVRMECHSKGRCPSSCPLCHVTSSPDTPAEPVLLEVTKAAPIYELVTNNQTQR
  LLQEATMSSLWCSGTGDVIEDWCRCDSTAFGADGLPTCAPLPQPVLRLSTVHEPSSTLVVLEWEHSEPPI
  GVQIVDYLLRQEKVTDRMDHSKVETETVLSFVDDIISGAKSPCAMPSQVPDKQLTTISLIIRCLEPDTIY
  MFTLWGVDNTGRRSRPSDVIVKTPCPVVDDVKAQEIADKIYNLFNGYTSGKEQQTAYNTLLDLGSPTLHR
  VLYHYNQHYESFGEFTWRCEDELGPRLPACWDSQRSCR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286164
• ORF Size: 3684 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286164.1, NP_001273093.1
• RefSeq Size: 4221 bp
• RefSeq ORF: 3687 bp
• Locus ID: 460
• Cytogenetics: 1q25.2
• Protein Families: Transmembrane
• MW: 136.9 kDa
• Gene Summary: 'Astrotactin is a neuronal adhesion molecule required for glial-guided migration of young postmitotic neuroblasts in cortical regions of developing brain, including cerebrum, hippocampus, cerebellum, and olfactory bulb (Fink et al., 1995).[supplied by OMIM, Jun 2009]'