TRPS1 (NM_001282903) Human Tagged ORF Clone

CAT#: RC240097

TRPS1 (myc-DDK-tagged) - Human trichorhinophalangeal syndrome I (TRPS1), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TRPS1
• Synonyms: GC79; LGCR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC240097 representing NM_001282903
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTCAATGCTGGATATGGTCCGGAAAAAGAACCCCCCTCTGAGAAACGTTGCAAGTGAAGGCGAGG
  GCCAGATCCTGGAGCCTATAGGTACAGAAAGCAAGGTATCTGGAAAGAACAAAGAATTTTCTGCAGATCA
  GATGTCAGAAAATACGGATCAGAGTGATGCTGCAGAACTAAATCATAAGGAGGAACATAGCTTGCATGTT
  CAAGATCCATCTTCTAGCAGTAAGAAGGACTTGAAAAGCGCAGTTCTGAGTGAGAAGGCTGGCTTCAATT
  ATGAAAGCCCCAGTAAGGGAGGAAACTTTCCCTCCTTTCCGCATGATGAGGTGACAGACAGAAATATGTT
  GGCTTTCTCATCTCCAGCTGCTGGGGGAGTCTGTGAGCCCTTGAAGTCTCCGCAAAGAGCAGAGGCAGAT
  GACCCTCAAGATATGGCCTGCACCCCCTCAGGGGACTCACTGGAGACAAAGGAAGATCAGAAGATGTCAC
  CAAAGGCTACAGAGGAAACAGGGCAAGCACAGAGTGGTCAAGCCAATTGTCAAGGTTTGAGCCCAGTTTC
  AGTGGCCTCAAAAAACCCACAAGTGCCTTCAGATGGGGGTGTAAGACTGAATAAATCCAAAACTGACTTA
  CTGGTGAATGACAACCCAGACCCGGCACCTCTGTCTCCAGAGCTTCAGGACTTTAAATGCAATATCTGTG
  GATATGGTTACTACGGCAACGACCCCACAGATCTGATTAAGCACTTCCGAAAGTATCACTTAGGACTGCA
  TAACCGCACCAGGCAAGATGCTGAGCTGGACAGCAAAATCTTGGCCCTTCATAACATGGTGCAGTTCAGC
  CATTCCAAAGACTTCCAGAAGGTCAACCGTTCTGTGTTTTCTGGTGTGCTGCAGGACATCAATTCTTCAA
  GGCCTGTTTTACTAAATGGGACCTATGATGTGCAGGTGACTTCAGGTGGAACATTCATTGGCATTGGACG
  GAAAACACCAGATTGCCAAGGGAACACCAAGTATTTCCGCTGTAAATTCTGCAATTTCACTTATATGGGC
  AACTCATCCACCGAATTAGAACAACATTTTCTTCAGACTCACCCAAACAAAATAAAAGCTTCTCTCCCCT
  CCTCTGAGGTTGCAAAACCTTCAGAGAAAAACTCTAACAAGTCCATCCCTGCACTTCAATCCAGTGATTC
  TGGAGACTTGGGAAAATGGCAGGACAAGATAACAGTCAAAGCAGGAGATGACACTCCTGTTGGGTACTCA
  GTGCCCATAAAGCCCCTCGATTCCTCTAGACAAAATGGTACAGAGGCCACCAGTTACTACTGGTGTAAAT
  TTTGTAGTTTCAGCTGTGAGTCATCTAGCTCACTTAAACTGCTAGAACATTATGGCAAGCAGCACGGAGC
  AGTGCAGTCAGGCGGCCTTAATCCAGAGTTAAATGATAAGCTTTCCAGGGGCTCTGTCATTAATCAGAAT
  GATCTAGCCAAAAGTTCAGAAGGAGAGACAATGACCAAGACAGACAAGAGCTCGAGTGGGGCTAAAAAGA
  AGGACTTCTCCAGCAAGGGAGCCGAGGATAATATGGTAACGAGCTATAATTGTCAGTTCTGTGACTTCCG
  ATATTCCAAAAGCCATGGCCCTGATGTAATTGTAGTGGGGCCACTTCTCCGTCATTATCAACAGCTCCAT
  AACATTCACAAGTGTACCATTAAACACTGTCCATTCTGTCCCAGAGGACTTTGCAGCCCAGAAAAGCACC
  TTGGAGAAATTACTTATCCGTTTGCTTGTAGAAAAAGTAATTGTTCCCACTGTGCACTCTTGCTTCTGCA
  CTTGTCTCCTGGGGCGGCTGGAAGCTCGCGAGTCAAACATCAGTGCCATCAGTGTTCATTCACCACCCCT
  GACGTAGATGTACTCCTCTTTCACTATGAAAGTGTGCATGAGTCCCAAGCATCGGATGTCAAACAAGAAG
  CAAATCACCTGCAAGGATCGGATGGGCAGCAGTCTGTCAAGGAAAGCAAAGAACACTCATGTACCAAATG
  TGATTTTATTACCCAAGTGGAAGAAGAGATTTCCCGACACTACAGGAGAGCACACAGCTGCTACAAATGC
  CGTCAGTGCAGTTTTACAGCTGCCGATACTCAGTCACTACTGGAGCACTTCAACACTGTTCACTGCCAGG
  AACAGGACATCACTACAGCCAACGGCGAAGAGGACGGTCATGCCATATCCACCATCAAAGAGGAGCCCAA
  AATTGACTTCAGGGTCTACAATCTGCTAACTCCAGACTCTAAAATGGGAGAGCCAGTTTCTGAGAGTGTG
  GTGAAGAGAGAGAAGCTGGAAGAGAAGGACGGGCTCAAAGAGAAAGTTTGGACCGAGAGTTCCAGTGATG
  ACCTTCGCAATGTGACTTGGAGAGGGGCAGACATCCTGCGGGGGAGTCCGTCATACACCCAAGCAAGCCT
  GGGGCTGCTGACGCCTGTGTCTGGCACCCAAGAGCAGACAAAGACTCTAAGGGATAGTCCCAATGTGGAG
  GCCGCCCATCTGGCGCGACCTATTTATGGCTTGGCTGTGGAAACCAAGGGATTCCTGCAGGGGGCGCCAG
  CTGGCGGAGAGAAGTCTGGGGCCCTCCCCCAGCAGTATCCTGCATCGGGAGAAAACAAGTCCAAGGATGA
  ATCCCAGTCCCTGTTACGGAGGCGTAGAGGCTCCGGTGTTTTTTGTGCCAATTGCCTGACCACAAAGACC
  TCTCTCTGGCGAAAGAATGCAAATGGCGGATATGTATGCAACGCGTGTGGCCTCTACCAGAAGCTTCACT
  CGACTCCCAGGCCTTTAAACATCATTAAACAAAACAACGGTGAGCAGATTATTAGGAGGAGAACAAGAAA
  GCGCCTTAACCCAGAGGCACTTCAGGCTGAGCAGCTCAACAAACAGCAGAGGGGCAGCAATGAGGAGCAA
  GTCAATGGAAGCCCGTTAGAGAGGAGGTCAGAAGATCATCTAACTGAAAGTCACCAGAGAGAAATTCCAC
  TCCCCAGCCTAAGTAAATACGAAGCCCAGGGTTCATTGACTAAAAGCCATTCTGCTCAGCAGCCAGTCCT
  GGTCAGCCAAACTCTGGATATTCACAAAAGGATGCAACCTTTGCACATTCAGATAAAAAGTCCTCAGGAA
  AGTACTGGAGATCCAGGAAATAGTTCATCCGTATCTGAAGGGAAAGGAAGTTCTGAGAGAGGCAGTCCTA
  TAGAAAAGTACATGAGACCTGCGAAACACCCAAATTATTCACCACCAGGCAGCCCTATTGAAAAGTACCA
  GTACCCACTTTTTGGACTTCCCTTTGTACATAATGACTTCCAGAGTGAAGCTGATTGGCTGCGGTTCTGG
  AGTAAATATAAGCTCTCCGTTCCTGGGAATCCGCACTACTTGAGTCACGTGCCTGGCCTACCAAATCCTT
  GCCAAAACTATGTGCCTTATCCCACCTTCAATCTGCCTCCTCATTTTTCAGCTGTTGGATCAGACAATGA
  CATTCCTCTAGATTTGGCGATCAAGCATTCCAGACCTGGGCCAACTGCAAACGGTGCCTCCAAGGAGAAA
  ACGAAGGCACCACCAAATGTAAAAAATGAAGGTCCCTTGAATGTAGTAAAAACAGAGAAAGTTGATAGAA
  GTACTCAAGATGAACTTTCAACAAAATGTGTGCACTGTGGCATTGTCTTTCTGGATGAAGTGATGTATGC
  TTTGCATATGAGTTGCCATGGTGACAGTGGACCTTTCCAGTGCAGCATATGCCAGCATCTTTGCACGGAC
  AAATATGACTTCACAACACATATCCAGAGGGGCCTGCATAGGAACAATGCACAAGTGGAAAAAAATGGAA
  AACCTAAAGAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC240097 representing NM_001282903
  Red=Cloning site Green=Tags(s)
  
  MKSMLDMVRKKNPPLRNVASEGEGQILEPIGTESKVSGKNKEFSADQMSENTDQSDAAELNHKEEHSLHV
  QDPSSSSKKDLKSAVLSEKAGFNYESPSKGGNFPSFPHDEVTDRNMLAFSSPAAGGVCEPLKSPQRAEAD
  DPQDMACTPSGDSLETKEDQKMSPKATEETGQAQSGQANCQGLSPVSVASKNPQVPSDGGVRLNKSKTDL
  LVNDNPDPAPLSPELQDFKCNICGYGYYGNDPTDLIKHFRKYHLGLHNRTRQDAELDSKILALHNMVQFS
  HSKDFQKVNRSVFSGVLQDINSSRPVLLNGTYDVQVTSGGTFIGIGRKTPDCQGNTKYFRCKFCNFTYMG
  NSSTELEQHFLQTHPNKIKASLPSSEVAKPSEKNSNKSIPALQSSDSGDLGKWQDKITVKAGDDTPVGYS
  VPIKPLDSSRQNGTEATSYYWCKFCSFSCESSSSLKLLEHYGKQHGAVQSGGLNPELNDKLSRGSVINQN
  DLAKSSEGETMTKTDKSSSGAKKKDFSSKGAEDNMVTSYNCQFCDFRYSKSHGPDVIVVGPLLRHYQQLH
  NIHKCTIKHCPFCPRGLCSPEKHLGEITYPFACRKSNCSHCALLLLHLSPGAAGSSRVKHQCHQCSFTTP
  DVDVLLFHYESVHESQASDVKQEANHLQGSDGQQSVKESKEHSCTKCDFITQVEEEISRHYRRAHSCYKC
  RQCSFTAADTQSLLEHFNTVHCQEQDITTANGEEDGHAISTIKEEPKIDFRVYNLLTPDSKMGEPVSESV
  VKREKLEEKDGLKEKVWTESSSDDLRNVTWRGADILRGSPSYTQASLGLLTPVSGTQEQTKTLRDSPNVE
  AAHLARPIYGLAVETKGFLQGAPAGGEKSGALPQQYPASGENKSKDESQSLLRRRRGSGVFCANCLTTKT
  SLWRKNANGGYVCNACGLYQKLHSTPRPLNIIKQNNGEQIIRRRTRKRLNPEALQAEQLNKQQRGSNEEQ
  VNGSPLERRSEDHLTESHQREIPLPSLSKYEAQGSLTKSHSAQQPVLVSQTLDIHKRMQPLHIQIKSPQE
  STGDPGNSSSVSEGKGSSERGSPIEKYMRPAKHPNYSPPGSPIEKYQYPLFGLPFVHNDFQSEADWLRFW
  SKYKLSVPGNPHYLSHVPGLPNPCQNYVPYPTFNLPPHFSAVGSDNDIPLDLAIKHSRPGPTANGASKEK
  TKAPPNVKNEGPLNVVKTEKVDRSTQDELSTKCVHCGIVFLDEVMYALHMSCHGDSGPFQCSICQHLCTD
  KYDFTTHIQRGLHRNNAQVEKNGKPKE
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282903
• ORF Size: 3861 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282903.1, NM_001282903.2, NP_001269832.1
• RefSeq Size: 10003 bp
• RefSeq ORF: 3864 bp
• Locus ID: 7227
• Cytogenetics: 8q23.3
• Protein Families: Druggable Genome, Transcription Factors
• MW: 142.7 kDa
• Gene Summary: 'This gene encodes a transcription factor that represses GATA-regulated genes and binds to a dynein light chain protein. Binding of the encoded protein to the dynein light chain protein affects binding to GATA consensus sequences and suppresses its transcriptional activity. Defects in this gene are a cause of tricho-rhino-phalangeal syndrome (TRPS) types I-III. [provided by RefSeq, Jul 2008]'