TRIP12 (NM_001284216) Human Tagged ORF Clone

CAT#: RC240204

TRIP12 (myc-DDK-tagged) - Human thyroid hormone receptor interactor 12 (TRIP12), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TRIP12
• Synonyms: MRD49; TRIP-12; ULF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC240204 representing NM_001284216
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCAACCGGCCTAATAACAATCCAGGGGGGTCACTGCGACGTTCACAGAGGAACACTGCCGGGGCCC
  AACCACAAGACGACTCAATAGGAGGAAGTCGGCGAGGCTCTGGCCTGGGCAAAAGAGGAGCAGCTGAAGC
  TCGTCGACAGGAGAAAATGGCAGACCCTGAAAGCAACCAGGAGGCAGTAAATTCTTCAGCTGCTCGGACA
  GATGAAGCTCCCCAAGGAGCTGCAGCCTCTAGTTCTGTTGCAGGGGCTGTTGGCATGACCACCTCTGGGG
  AGAGTGAATCAGATGATTCCGAGATGGGACGTTTGCAAGCTTTGTTAGAGGCAAGGGGTCTTCCCCCTCA
  CCTATTTGGTCCTCTTGGTCCTCGGATGTCACAGCTTTTCCATAGAACAATTGGAAGTGGAGCTAGTTCT
  AAGGCCCAGCAGCTACTACAAGGATTGCAAGCCAGTGATGAAAGTCAACAGCTTCAGGCAGTTATTGAGA
  TGTGTCAGTTACTGGTCATGGGAAATGAGGAGACACTGGGAGGGTTTCCTGTCAAGAGTGTTGTTCCAGC
  TTTGATTACGTTACTTCAGATGGAGCACAATTTTGATATTATGAACCATGCTTGTCGAGCCTTAACATAC
  ATGATGGAAGCACTTCCTCGATCTTCTGCTGTTGTAGTAGATGCTATTCCTGTCTTTTTAGAAAAGCTGC
  AAGTTATTCAGTGTATTGATGTGGCAGAGCAGGCCTTGACTGCCTTGGAGATGTTGTCACGGAGACATAG
  TAAAGCCATTCTACAGGCGGGTGGTTTGGCAGACTGCTTGCTGTACCTAGAATTCTTCAGCATAAATGCC
  CAAAGAAATGCATTAGCAATTGCAGCTAATTGCTGCCAGAGTATCACGCCAGATGAATTTCATTTTGTGG
  CAGATTCACTCCCATTGCTAACCCAAAGGCTAACACATCAGGATAAAAAGTCAGTAGAAAGCACTTGCCT
  TTGTTTTGCACGCCTAGTGGACAACTTCCAGCATGAGGAGAATTTACTCCAGCAGGTTGCTTCCAAAGAT
  CTGCTTACAAATGTTCAACAGCTGTTGGTAGTGACTCCACCCATTTTAAGTTCTGGGATGTTTATAATGG
  TGGTTCGCATGTTTTCTCTGATGTGTTCCAACTGTCCAACTTTAGCTGTTCAACTTATGAAACAAAACAT
  TGCAGAAACGCTTCACTTTCTCCTGTGTGGTGCCTCCAATGGAAGTTGTCAGGAACAGATTGATCTTGTT
  CCACGAAGCCCTCAAGAGTTGTATGAACTGACATCTCTGATTTGTGAACTTATGCCATGTTTACCAAAAG
  AAGGCATTTTTGCAGTTGATACCATGTTGAAGAAGGGAAATGCACAGAACACAGATGGTGCGATATGGCA
  GTGGCGTGATGATCGGGGCCTCTGGCATCCATATAACAGGATTGACAGCCGGATCATTGAGGCAGCCCAT
  CAGGTCGGTGAGGATGAGATAAGCTTGTCCACTCTGGGACGAGTTTATACTATTGATTTTAATTCTATGC
  AGCAAATCAATGAGGACACGGGAACAGCACGTGCCATTCAGAGAAAACCTAACCCGTTAGCCAATAGTAA
  CACTAGTGGATATTCAGAGTCAAAGAAGGATGATGCTCGAGCACAGCTTATGAAAGAGGATCCGGAACTG
  GCTAAGTCTTTTATTAAGACATTATTTGGTGTTCTTTATGAAGTGTATAGTTCCTCAGCAGGACCTGCGG
  TCAGACATAAGTGCCTTAGAGCAATTCTTAGGATAATTTATTTTGCGGATGCTGAACTTCTGAAGGATGT
  TCTGAAAAATCATGCTGTTTCAAGTCACATTGCTTCCATGCTGTCAAGCCAAGACCTGAAGATAGTAGTG
  GGAGCACTTCAGATGGCAGAAATTTTAATGCAGAAGTTACCTGATATTTTTAGTGTTTACTTCAGAAGAG
  AAGGTGTAATGCATCAAGTAAAACACTTAGCAGAATCAGAGTCTTTGTTGACAAGTCCACCAAAGGCATG
  TACGAATGGATCGGGATCCATGGGATCCACAACTTCAGTCAGCAGTGGGACAGCCACAGCTGCCACTCAT
  GCTGCAGCTGACTTGGGATCACCCAGCTTGCAGCACAGCAGGGATGATTCTTTAGATCTCAGCCCTCAAG
  GTCGATTAAGTGATGTTCTAAAGAGAAAACGACTGCCAAAACGAGGGCCAAGAAGGCCAAAGTACTCACC
  TCCAAGAGATGATGACAAAGTAGACAATCAAGCTAAAAGCCCCACCACTACTCAGTCACCTAAATCTTCT
  TTCCTGGCAAGCTTGAATCCAAAAACATGGGGAAGGTTAAGTACACAGTCCAACAGCAACAACATTGAGC
  CAGCACGGACTGCGGGAGGTAGTGGCCTTGCCAGGGCTGCCTCAAAGGATACCATCTCCAATAATAGAGA
  AAAAATTAAAGGTTGGATTAAGGAGCAGGCACATAAATTTGTAGAACGTTATTTCAGTTCTGAGAATATG
  GATGGAAGCAACCCTGCATTGAATGTCCTTCAGAGACTTTGTGCTGCAACCGAACAACTCAACCTCCAGG
  TGGATGGTGGAGCTGAGTGCCTTGTAGAAATCCGTAGCATAGTCTCAGAGTCAGATGTTTCATCATTTGA
  AATCCAACATAGTGGATTTGTGAAGCAGCTGTTGCTTTATTTGACATCTAAAAGTGAAAAGGATGCTGTG
  AGCAGAGAGATCAGATTAAAGCGATTTCTTCATGTATTTTTTTCTTCTCCACTTCCTGGAGAAGAGCCCA
  TTGGAAGAGTGGAACCAGTGGGTAATGCACCTTTGTTGGCATTAGTTCACAAGATGAACAACTGCCTCAG
  CCAGATGGAACAATTTCCAGTCAAAGTACATGATTTCCCTAGTGGAAATGGGACAGGAGGCAGCTTTTCT
  CTCAACAGAGGATCACAGGCTTTAAAATTTTTCAACACACATCAATTAAAATGCCAGTTACAAAGGCATC
  CAGACTGTGCAAATGTGAAGCAGTGGAAGGGTGGACCTGTCAAGATTGACCCTCTGGCTTTGGTACAAGC
  CATCGAGAGATACCTTGTAGTTAGAGGGTATGGAAGAGTAAGAGAAGATGATGAAGACAGCGATGACGAT
  GGATCAGATGAGGAAATAGATGAGTCTCTGGCTGCTCAGTTCCTAAATTCAGGAAATGTAAGACACAGGC
  TGCAGTTTTATATTGGAGAACATTTGCTGCCGTATAACATGACTGTGTATCAGGCAGTACGGCAGTTTAG
  TATACAGGCTGAAGATGAAAGAGAATCCACAGATGATGAGAGCAATCCTCTAGGCAGAGCTGGTATTTGG
  ACAAAGACTCATACAATATGGTATAAACCTGTGAGAGAGGATGAAGAAAGTAATAAAGATTGTGTTGGTG
  GTAAAAGAGGAAGAGCCCAAACAGCTCCAACGAAAACTTCCCCTAGAAATGCAAAAAAGCATGATGAGTT
  ATGGCACGATGGAGTGTGCCCATCAGTATCAAATCCTTTAGAAGTTTACCTCATTCCCACACCACCTGAA
  AATATAACATTTGAAGACCCGTCATTAGATGTGATCCTTCTTTTAAGAGTTTTACATGCTATCAGTCGAT
  ACTGGTATTACTTGTATGATAATGCAATGTGCAAGGAAATTATTCCAACTAGTGAATTTATTAACAGTAA
  GTTAACAGCAAAAGCAAATAGGCAACTTCAAGATCCTTTAGTAATCATGACAGGAAACATCCCAACATGG
  CTTACTGAGCTAGGAAAAACCTGCCCATTTTTCTTTCCTTTTGATACCCGGCAAATGCTTTTTTATGTAA
  CTGCATTTGATCGGGACCGAGCAATGCAAAGATTACTTGATACCAACCCAGAAATCAACCAGTCTGATTC
  TCAAGATAGCAGAGTTGCACCTAGATTGGATAGAAAAAAACGTACTGTGAACCGAGAGGAGCTGCTGAAA
  CAGGCGGAGTCTGTGATGCAGGACCTCGGCAGCTCACGGGCCATGTTAGAAATCCAGTATGAAAATGAGG
  TTGGTACAGGTCTTGGGCCTACACTGGAGTTTTATGCGCTTGTATCTCAGGAACTACAGAGAGCTGACTT
  GGGTCTTTGGAGAGGTGAAGAAGTAACTCTTAGCAATCCAAAAGGGAGCCAAGAAGGGACCAAGTATATT
  CAAAACCTCCAGGGCCTGTTTGCGCTTCCCTTTGGTAGGACAGCAAAGCCAGCTCATATCGCAAAGGTTA
  AGATGAAGTTTCGCTTCTTAGGAAAATTAATGGCCAAGGCTATCATGGATTTCAGATTGGTGGACCTTCC
  CCTTGGCTTACCCTTTTATAAATGGATGCTACGGCAAGAAACTTCACTGACATCACACGATTTGTTTGAC
  ATCGACCCAGTTGTAGCCAGATCAGTTTATCACCTAGAAGACATTGTCAGACAGAAGAAAAGACTTGAAC
  AAGATAAATCCCAGACCAAAGAGAGTCTACAGTATGCATTAGAAACCTTGACTATGAATGGCTGCTCAGT
  TGAAGATCTAGGACTGGATTTCACTCTGCCAGGGTTTCCCAATATCGAACTGAAGAAAGGAGGGAAGGAT
  ATACCAGTCACTATCCACAATTTAGAGGAGTATCTAAGACTGGTTATATTCTGGGCACTAAATGAAGGCG
  TTTCTAGGCAATTTGATTCGTTCAGAGATGGATTTGAATCAGTCTTCCCACTCAGTCATCTTCAGTACTT
  CTACCCGGAGGAACTGGATCAGCTCCTTTGTGGCAGTAAAGCAGACACTTGGGATGCAAAGACACTGATG
  GAATGCTGTAGGCCTGATCATGGTTATACTCATGACAGTCGGGCTGTGAAGTTTTTGTTTGAGATTCTCA
  GTAGTTTTGATAATGAGCAGCAGAGGTTATTTCTCCAGTTTGTGACTGGTAGCCCAAGATTGCCTGTTGG
  AGGATTCCGGAGTTTGAATCCACCTTTGACAATTGTCCGAAAGACGTTTGAATCAACAGAAAACCCAGAT
  GACTTCTTGCCCTCTGTAATGACTTGTGTGAACTATCTTAAGTTGCCGGACTATTCAAGCATTGAGATAA
  TGCGTGAAAAACTGTTGATAGCAGCAAGAGAAGGGCAGCAGTCGTTCCATCTTTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC240204 representing NM_001284216
  Red=Cloning site Green=Tags(s)
  
  MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAART
  DEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASS
  KAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTY
  MMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINA
  QRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKD
  LLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLV
  PRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAH
  QVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPEL
  AKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVV
  GALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATH
  AAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSS
  FLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENM
  DGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAV
  SREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFS
  LNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDD
  GSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIW
  TKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPE
  NITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTW
  LTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLK
  QAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYI
  QNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFD
  IDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKD
  IPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLM
  ECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPD
  DFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001284216
• ORF Size: 5166 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001284216.1, NP_001271145.1
• RefSeq Size: 9082
• RefSeq ORF: 5169
• Locus ID: 9320
• Protein Families: Druggable Genome
• Protein Pathways: Ubiquitin mediated proteolysis
• MW: 192.5 kDa
• Gene Summary: The protein encoded by this gene is an E3 ubiquitin-protein ligase involved in the degradation of the p19ARF/ARF isoform of CDKN2A, a tumor suppressor. The encoded protein also plays a role in the DNA damage response by regulating the stability of USP7, which regulates tumor suppressor p53. [provided by RefSeq, Jan 2017]