GARNL1 (RALGAPA1) (NM_001283043) Human Tagged ORF Clone

CAT#: RC240229

RALGAPA1 (myc-DDK-tagged) - Human Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RALGAPA1
• Synonyms: GARNL1; GRIPE; p240; RalGAPalpha1; TULIP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC240229 representing NM_001283043
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCTCCAAGAAGCCGCACGGGGACGTGAAGAAGTCCACCCAGAAGGTGCTAGACACCAAGAAGGACG
  CACTGACTCGCCTCAAGCACCTGCGCATCGTCATCGAGAATGCAGAATCTATTGATCTTAAACAGTTTTT
  CGACCAACATTTTTCACATATATACTATGTGTTCTTTGAAAATTTTGTGACTATTGAAGCTAGTCTTAAA
  CAGAAAGGTCACAAGTCTCAAAGAGAGGAATTGGATGCTATACTTTTTATTTTTGAGAAAATTTTACAAC
  TTCTTCCAGAAAGAATTCATCAGCGATGGCAGTTTCATAGTATTGGATTGATTTTAAAGAAGCTACTTCA
  CACAGGAAACTCCTTAAAGATTAGGCGGGAAGGTGTTCGTCTTTTCTTACTATGGTTGCAAGCTCTTCAG
  AATAACTGTAGCAAAGAACAGCTCTGGATGTTTTCATGCTTAATCCCTGGATTTTCAGCACCACAGTCTG
  AACATGGACCTCGAACTTTAGATAATCTCATTAATCCTCCACTCAACCTTCAAGAAACTCAAGTCACTAT
  AGAAGAAATCACTCCTCTTGTCCCCCCACAATCAGGAGATAAAGGGCAAGAAGATCTCACAAGCTATTTT
  CTTGAAGCACTTCTAAAATACATAGTCATTCAGGTAAAAAGTTTAGAATGGAAGAACAAAGAAAACCAAG
  AAAGGGGATTTTCATTTTTGTTTTCACATTTTAAGAAATATTACTTGCCTTATATTTTTCCAAACATCTG
  TAAGGAAAACAGTTTATATCATCCTATACTTGACATCCCGCAGATGAGACCAAAGCCACATTATGTCGTG
  ATAAAGAAAGATGCTGAAACCAATGAAGCAATCTATTGTACAAAGGAGCCTTTCATTAAGGCTCGTGTTA
  TTGTCATTCGTTGGCTGGTTTCTTTCTGGCTGGAGCCAAAACCACATACAGGACCTCATATTCCTGGGAT
  GGAAGGTGAAGTCTTGCCAAAGAATATTCAGAGAGCAGCTGCTAGTTTAGTATCCAGAGAAGAAAGCAAA
  AATGATAATGCTGATAAAACAGACAGAACTACAGAACCCGAACAGTCTCATTCCAATACAAGCACTCTCA
  CGGAGCGAGAACCTAGCTCATCTAGTCTCTGTAGTATTGATGAAGAACATCTCACAGACATTGAAATAGT
  TCGCAGAGTTTTTTCTTCTAAAAGGAGTAATGTAAACTTTGTGACAGAGATATTTCGTCAGGCATTTTTA
  TTACCAATTTGTGAAGCAGCAGCTATGAGAAAAGTGGTAAAAGTATATCAAGAATGGATCCAACAAGAGG
  AAAAACCTTTGTTCATGCAAGAGCCTGAAGAAATTGTGATCACTTCTTCAGACCTCCCTTGCATTGAAAA
  TGTCACAGACCATGATATTTCAATGGAAGAAGGAGAAAAAAGAGAAGAGGAAAATGGGACCAATACTGCT
  GATCATGTTCGAAATTCCAGTTGGGCAAAAAACGGCTCCTACCAAGGTGCTCTTCATAACGCCTCTGAAG
  AAGCCACAGAACAAAACATACGAGCTGGTACCCAGGCAGTTTTGCAGGTGTTTATTATAAACTCATCAAA
  TATATTTCTTCTTGAACCTGCAAATGAAATAAAAAATCTTCTGGATGAACACACAGATATGTGTAAACGC
  ATTCTTAACATTTATCGGTACATGGTTGTACAAGTATCAATGGACAAAAAGACTTGGGAACAGATGCTGC
  TTGTGTTGCTCAGAGTCACGGAATCTGTACTGAAGATGCCATCACAAGCTTTTCTACAGTTCCAAGGGAA
  AAAAAATATGACCTTGGCAGGTCGACTTGCAGGACCACTTTTCCAGACCCTTATAGTTGCCTGGATCAAA
  GCAAACCTAAATGTGTACATCTCCCGAGAACTTTGGGATGACTTACTGTCAGTATTGTCATCGCTGACCT
  ATTGGGAAGAGTTGGCCACTGAGTGGTCACTGACTATGGAGACATTAACTAAAGTTTTAGCTAGGAATTT
  ATATAGTTTGGATCTCAGTGATTTACCATTGGATAAGCTGAGTGAACAGAAACAAAAAAAGCACAAAGGG
  AAAGGAGTTGGACATGAATTTCAGAAAGTTTCAGTTGACAAGTCATTTTCTAGAGGATGGAGTCGTGATC
  AGCCTGGCCAAGCCCCAATGAGACAGAGGAGTGCAACAACCACTGGTTCTCCAGGAACCGAAAAGGCGAG
  GAGTATAGTACGGCAAAAAACTGTCGATATTGATGATGCTCAAATACTTCCCCGCTCAACTAGAGTCAGA
  CATTTTTCACAAAGTGAAGAAACTGGAAATGAAGTTTTTGGTGCTTTGAATGAGGAGCAGCCATTGCCTC
  GAAGTAGCAGCACTTCTGACATCTTGGAACCATTCACTGTTGAACGAGCCAAAGAGGCAGAACAGAATGC
  CACCAGAGGCTCCACTGAGGGATCTGTACAGTCATGTAATGGGCTATTTTGGAAGGAAAGCTGCAGTGAT
  ATTGGCGGCAGCAGTGCTAATGTTCCTGATCTGATGGATGAGTTTATAGCAGAACGACTTCGAAGTGGTA
  ATGCCTCGACTATGACAAGAAGAGGAAGTAGTCCAGGCAGCCTGGAAATTCCCAAAGACCTCCCTGATAT
  TCTAAACAAGCAGAACCAGATGCGCCCTATTGATGACCCAGGTGTGCCCTCAGAATGGACTTCTCCTGCC
  AGTGCAGGGAGCAGTGATCTTATCAGCTCAGATAGTCATTCGGATTCTTTCAGCGCTTTCCAATATGATG
  GCCGAAAATTTGACAATTTTGGCTTTGGAACCGACACTGGGGTTACGTCCTCTGCTGATGTGGATTCAGG
  TTCTGGCCATCATCAGAGTGCTGAAGAGCAGGAAGTGGCTAGTCTAACTACTCTTCATATAGATTCTGAA
  ACAAGCAGTCTTAATCAGCAAGCTTTCTCTGCTGAAGTTGCAACTATTACTGGTTCAGAAAGTGCTTCCC
  CAGTCCACTCACCTCTGGGCTCCAGGTCACAGACTCCTTCCCCTTCTACATTGAATATAGATCACATGGA
  ACAGAAGGATCTGCAGCTCGACGAGAAGCTCCACCACTCTGTTCTTCAGACACCAGATGATCTAGAAATT
  AGTGAATTTCCATCAGAATGTTGTAGTGTGATGGCAGGAGGTACTCTGACTGGATGGCATGCTGATGTTG
  CTACTGTAATGTGGCGAAGAATGCTAGGCATTTTGGGAGATGTAAATTCAATCATGGATCCTGAAATACA
  TGCTCAAGTTTTTGATTACCTCTGTGAACTTTGGCAGAATCTAGCTAAGATTAGAGATAACCTTGGCATT
  TCAACTGATAACCTGACCTCCCCTTCTCCACCAGTTTTAATTCCTCCACTGAGAATTCTTACACCTTGGC
  TTTTTAAGGCAACCATGTTGACTGATAAATATAAACAAGGTAAATTACATGCATATAAACTTATTTGTAA
  TACAATGAAAAGAAGACAAGATGTTTCTCCAAATAGAGATTTTCTAACACATTTCTACAATATAATGCAT
  TGTGGATTACTTCATATTGACCAGGATATTGTCAATACAATCATCAAACACTGCTCACCTCAATTTTTTT
  CACTTGGTTTGCCTGGTGCCACAATGCTTATTATGGATTTTATTGTAGCAGCTGGTAGAGTGGCTTCTTC
  AGCTTTTCTCAATGCACCAAGAGTAGAAGCACAAGTTCTTCTGGGATCTTTGGTTTGCTTTCCCAACTTA
  TATTGTGAACTGCCTTCTCTTCATCCCAACATTCCTGATGTTGCTGTGTCTCAGTTTACAGATGTTAAGG
  AACTTATAATCAAAACTGTATTAAGCTCGGCAAGAGATGAGCCCTCTGGTCCTGCACGATGTGTAGCACT
  TTGTAGTTTAGGTATTTGGATTTGTGAAGAACTAGTCCATGAGTCTCATCATCCTCAAATTAAGGAAGCT
  CTGAATGTGATTTGTGTTTCCTTAAAGTTTACTAATAAAACAGTAGCCCACGTAGCTTGTAACATGCTTC
  ACATGCTGGTTCATTATGTACCTAGACTTCAGATTTACCAGCCTGATTCTCCCTTGAAAATTATTCAAAT
  CCTAATAGCTACCATCACCCATCTTTTACCAAGTACAGAGGCTTCATCTTATGAAATGGACAAGAGGTTG
  GTAGTATCTTTACTTCTCTGCCTTCTGGACTGGATCATGGCCTTACCTCTAAAGACACTGCTCCAACCAT
  TTCATGCTACGGGAGCAGAAAGCGATAAAACAGAAAAATCTGTTCTCAATTGCATTTATAAGGTTTTACA
  TGGGTGTGTTTATGGAGCTCAGTGTTTTAGCAATCCAAGGTATTTTCCCATGAGCCTCTCTGATTTGGCA
  TCTGTAGATTATGATCCTTTTATGCATTTGGAAAGTCTGAAAGAGCCTGAGCCTCTGCACTCTCCTGACT
  CAGAACGATCTTCTAAACTCCAGCCAGTAACAGAAGTGAAAACTCAAATGCAGCATGGATTAATCTCTAT
  AGCAGCCCGCACTGTTATTACACATCTGGTAAATCACCTGGGCCATTATCCAATGAGCGGTGGTCCTGCT
  ATGCTAACAAGTCAGGTGTGTGAAAATCACGACAATCATTACAGTGAAAGTACTGAACTTTCTCCTGAAC
  TCTTTGAGAGTCCAAATATCCAGTTCTTTGTGTTAAATAATACAACCTTAGTGTCCTGTATCCAGATCAG
  ATCAGAAGAGAATATGCCTGGAGGAGGTTTATCTGCTGGCCTTGCATCAGCCAATTCAAATGTCAGAATC
  ATAGTACGTGATCTCTCTGGAAAATATTCATGGGATTCTGCTATACTGTATGGCCCACCTCCTGTAAGTG
  GCTTGTCAGAACCTACATCTTTCATGCTTTCATTGTCTCACCAAGAGAAGCCAGAAGAGCCTCCGACATC
  TAATGAATGCTTAGAAGATATAACCGTAAAAGATGGACTTTCTCTCCAGTTTAAAAGATTTAGAGAAACT
  GTACCAACTTGGGATACAATAAGAGATGAAGAAGATGTTCTTGATGAGCTCTTGCAGTATTTGGGTGTTA
  CTAGTCCTGAATGCTTACAGAGAACTGGAATCTCACTTAATATTCCTGCTCCACAACCTGTGTGCATTTC
  TGAAAAACAAGAAAATGATGTTATTAATGCTATCCTTAAGCAACATACAGAAGAAAAAGAATTTGTTGAG
  AAGCACTTTAATGACTTAAACATGAAAGCTGTGGAACAAGATGAACCAATACCTCAAAAACCTCAGTCAG
  CATTTTATTATTGCAGATTGCTTCTTAGTATATTGGGAATGAATTCCTGGGACAAACGGAGGAGCTTTCA
  TCTCCTGAAGAAAAATGAAAAGCTACTTAGAGAACTTAGGAACTTGGATTCAAGGCAGTGCCGAGAGACA
  CACAAGATTGCAGTATTTTATGTTGCTGAAGGACAAGAAGACAAACACTCCATTCTCACCAATACAGGAG
  GAAGTCAAGCATATGAAGATTTTGTAGCTGGTCTTGGTTGGGAGGTAAATCTTACAAACCATTGTGGTTT
  TATGGGAGGACTACAAAAAAACAAAAGCACTGGATTGACCACTCCATATTTTGCTACCTCTACAGTAGAG
  GTAATATTTCACGTGTCAACAAGAATGCCTTCTGATTCTGATGATTCTTTGACCAAAAAATTGAGACATT
  TGGGAAATGATGAAGTGCACATTGTTTGGTCAGAGCATACTAGAGACTACAGGAGAGGAATTATTCCCAC
  AGAATTTGGTGATGTCCTTATTGTAATATATCCAATGAAAAATCACATGTTCAGTATTCAGATAATGAAA
  AAACCAGAGGTTCCCTTCTTTGGTCCCCTTTTTGATGGTGCTATTGTGAATGGAAAGGTTCTACCCATTA
  TGGTTAGAGCAACAGCTATAAATGCAAGCCGTGCTCTGAAATCTCTGATTCCATTGTATCAAAACTTCTA
  TGAGGAGAGAGCACGATACCTGCAAACAATTGTCCAGCACCACTTAGAACCAACAACATTTGAAGATTTT
  GCAGCACAGGTTTTTTCTCCAGCTCCCTACCACCATTTACCATCTGATGCCGATCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC240229 representing NM_001283043
  Red=Cloning site Green=Tags(s)
  
  MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLK
  QKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQ
  NNCSKEQLWMFSCLIPGFSAPQSEHGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYF
  LEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKKYYLPYIFPNICKENSLYHPILDIPQMRPKPHYVV
  IKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREESK
  NDNADKTDRTTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFL
  LPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVITSSDLPCIENVTDHDISMEEGEKREEENGTNTA
  DHVRNSSWAKNGSYQGALHNASEEATEQNIRAGTQAVLQVFIINSSNIFLLEPANEIKNLLDEHTDMCKR
  ILNIYRYMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIK
  ANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKG
  KGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRSTRVR
  HFSQSEETGNEVFGALNEEQPLPRSSSTSDILEPFTVERAKEAEQNATRGSTEGSVQSCNGLFWKESCSD
  IGGSSANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPA
  SAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGTDTGVTSSADVDSGSGHHQSAEEQEVASLTTLHIDSE
  TSSLNQQAFSAEVATITGSESASPVHSPLGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEI
  SEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGI
  STDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMH
  CGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNL
  YCELPSLHPNIPDVAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEA
  LNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRL
  VVSLLLCLLDWIMALPLKTLLQPFHATGAESDKTEKSVLNCIYKVLHGCVYGAQCFSNPRYFPMSLSDLA
  SVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQHGLISIAARTVITHLVNHLGHYPMSGGPA
  MLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRSEENMPGGGLSAGLASANSNVRI
  IVRDLSGKYSWDSAILYGPPPVSGLSEPTSFMLSLSHQEKPEEPPTSNECLEDITVKDGLSLQFKRFRET
  VPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGISLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVE
  KHFNDLNMKAVEQDEPIPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRET
  HKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVE
  VIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMK
  KPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDF
  AAQVFSPAPYHHLPSDADH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001283043
• ORF Size: 6147 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001283043.1, NM_001283043.2, NP_001269972.1
• RefSeq Size: 7921
• RefSeq ORF: 6150
• Locus ID: 253959
• MW: 231.6 kDa
• Gene Summary: This gene encodes a major subunit of the RAL-GTPase activating protein. A similar protein in mouse binds E12, a transcriptional regulator of immunoglobulin genes. The mouse protein also functions in skeletal muscle by binding to the regulatory 14-3-3 proteins upon stimulation with insulin or muscle contraction. A pseudogene of this gene has been identified on chromosome 9. [provided by RefSeq, Oct 2016]