Phospholipase C epsilon 1 (PLCE1) (NM_001288989) Human Tagged ORF Clone

CAT#: RC240241

PLCE1 (myc-DDK-tagged) - Human phospholipase C, epsilon 1 (PLCE1), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PLCE1
• Synonyms: NPHS3; PLCE; PPLC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC240241 representing NM_001288989
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTTCTGAAGAAATGACAGCTTCTGTTCTCATACCTGTGACTCAGAGAAAAGTGGTTTCTGCCCAGT
  CGGCTGCAGATGAAAGTAGTGAAAAGGTCTCAGACATCAATATTTCAAAAGCACATACTGTCAGACGAAG
  TGGGGAGACTTCTCATACCATCTCACAACTGAACAAACTTAAAGAAGAACCTTCTGGAAGCAACTTGCCA
  AAGATTCTCTCAATAGCGAGGGAGAAAATAGTGAGTGATGAGAACAGTAATGAAAAATGTTGGGAGAAAA
  TCATGCCAGATTCTGCGAAAAACCTTAACATTAACTGCAACAACATATTGAGAAACCATCAGCATGGCCT
  TCCTCAGAGACAATTTTATGAAATGTACAACTCTGTTGCTGAGGAAGACTTGTGTTTAGAAACTGGAATT
  CCTTCTCCACTGGAAAGAAAGGTGTTCCCTGGAATTCAACTGGAACTAGACAGACCTTCCATGGGCATTA
  GTCCTTTAGGAAATCAGTCAGTGATCATAGAGACAGGCAGAGCACACCCTGACAGCAGAAGGGCAGTATT
  TCATTTTCATTATGAAGTTGACAGAAGAATGTCAGACACTTTCTGTACCCTATCAGAAAACTTAATTTTA
  GACGATTGTGGAAATTGTGTACCACTACCTGGGGGTGAGGAGAAGCAAAAGAAAAACTATGTGGCATATA
  CCTGTAAACTGATGGAATTGGCCAAAAATTGTGATAATAAGAATGAGCAGCTGCAGTGTGATCATTGTGA
  CACCTTGAATGATAAATACTTTTGCTTTGAAGGCTCTTGTGAGAAGGTTGACATGGTATATTCAGGTGAT
  AGCTTTTGTAGGAAAGACTTTACTGACAGTCAAGCTGCCAAGACCTTTTTGAGCCATTTTGAGGACTTCC
  CTGATAATTGTGATGATGTAGAAGAAGACGCTTTTAAAAGCAAAAAGGAGCGATCCACTTTGTTAGTCAG
  GAGATTCTGTAAAAATGACAGAGAAGTTAAGAAATCTGTGTATACTGGAACAAGAGCAATTGTGAGAACT
  CTGCCTTCTGGCCACATTGGGCTGACTGCATGGAGTTACATAGATCAGAAGAGAAATGGTCCCTTACTGC
  CTTGTGGGAGAGTAATGGAACCCCCGTCAACAGTGGAGATAAGGCAAGATGGGAGCCAACGTCTGTCAGA
  AGCCCAGTGGTATCCTATCTACAATGCAGTGAGAAGAGAAGAAACAGAAAATACAGTTGGATCTCTACTC
  CATTTCCTCACCAAGCTCCCAGCCTCCGAGACAGCCCATGGAAGGATAAGCGTTGGTCCATGCTTAAAGC
  AATGTGTCCGAGACACTGTATGTGAGTATCGCGCCACCCTCCAAAGGACTTCAATATCGCAGTACATCAC
  CGGTTCTCTCCTAGAAGCAACCACGTCTTTGGGAGCAAGAAGTGGCCTTCTCAGTACTTTTGGAGGATCC
  ACTGGACGAATGATGCTGAAAGAACGCCAGCCAGGCCCCTCTGTGGCCAATTCCAATGCCCTCCCTTCAA
  GTTCAGCTGGGATCAGCAAGGAGCTGATCGATCTGCAGCCTCTCATCCAGTTCCCAGAGGAAGTCGCCAG
  CATCCTGATGGAGCAAGAGCAGACTATTTACCGCAGGGTCTTGCCAGTCGACTACCTTTGCTTCTTAACA
  CGGGACTTGGGCACTCCTGAATGCCAGAGCTCCTTGCCCTGCCTCAAAGCATCCATCTCAGCGTCGATTC
  TTACCACTCAGAATGGAGAGCACAATGCCCTTGAAGATCTGGTGATGAGGTTTAATGAGGTGAGCTCCTG
  GGTGACATGGCTGATCCTCACGGCAGGCTCCATGGAGGAGAAGCGAGAAGTCTTTTCATATTTGGTGCAT
  GTGGCCAAATGCTGCTGGAACATGGGCAACTACAACGCTGTCATGGAGTTCTTGGCTGGCCTCAGGTCAA
  GAAAAGTTTTAAAAATGTGGCAGTTCATGGACCAGTCTGATATTGAGACCATGAGGAGCCTGAAGGATGC
  TATGGCCCAGCATGAGTCCTCTTGTGAGTACAGAAAGGTGGTGACACGTGCCCTGCACATCCCTGGCTGT
  AAGGTGGTTCCATTCTGTGGGGTGTTTCTGAAGGAGCTCTGTGAAGTGCTTGACGGCGCCTCCGGTCTCA
  TGAAGCTTTGCCCGCGGTACAATTCCCAAGAAGAAACTTTAGAGTTTGTAGCAGATTACAGTGGACAAGA
  TAATTTCTTACAACGAGTGGGACAAAATGGCTTAAAGAATTCGGAGAAGGAGTCCACTGTCAACAGCATC
  TTTCAGGTCATCCGGAGCTGCAATCGAAGTCTGGAGACAGACGAGGAGGACAGCCCCAGTGAAGGAAACA
  GCTCCAGGAAAAGCTCCTTGAAGGATAAAAGCCGATGGCAGTTTATAATTGGAGATTTGTTGGATTCAGA
  CAATGACATCTTTGAGCAATCCAAAGAATACGACTCTCATGGTTCAGAGGACTCACAGAAGGCCTTCGAC
  CATGGGACGGAGCTCATCCCTTGGTACGTGCTGTCCATCCAAGCCGATGTGCACCAGTTCCTGCTGCAGG
  GGGCCACGGTCATCCACTACGACCAGGACACACACCTCTCTGCCCGCTGCTTCCTCCAGCTTCAGCCCGA
  CAATAGCACCTTGACCTGGGTAAAGCCCACAACTGCCTCCCCAGCCAGCAGTAAAGCAAAACTTGGTGTA
  CTTAATAACACAGCTGAGCCTGGAAAATTCCCACTACTGGGTAATGCTGGATTAAGTAGCCTGACGGAAG
  GGGTCTTGGATCTTTTTGCAGTGAAGGCTGTATACATGGGCCACCCTGGCATTGATATACACACTGTGTG
  TGTTCAGAACAAACTGGGTAGCATGTTCCTGTCAGAGACTGGTGTGACATTGCTCTATGGGCTTCAGACC
  ACAGACAACAGATTATTGCACTTCGTGGCACCAAAGCACACAGCTAAAATGCTCTTCAGCGGATTATTGG
  AACTCACTAGAGCTGTGAGAAAGATGAGGAAATTCCCTGACCAAAGACAGCAGTGGCTGCGGAAACAGTA
  CGTCAGCCTTTATCAGGAGGATGGACGGTATGAAGGCCCAACTTTGGCTCACGCTGTGGAGTTGTTTGGT
  GGCAGACGGTGGAGTGCTCGAAACCCCAGCCCCGGAACATCAGCAAAGAATGCTGAGAAGCCCAATATGC
  AGAGAAACAATACCCTGGGCATAAGCACTACCAAGAAAAAGAAGAAAATCCTCATGAGGGGTGAGAGTGG
  AGAGGTAACTGACGATGAGATGGCAACCCGAAAGGCCAAGATGCACAAAGAGTGTCGAAGCCGGAGTGGT
  TCTGATCCTCAAGACATTAATGAACAAGAAGAATCAGAGGTGAATGCCATCGCTAACCCTCCAAACCCCC
  TCCCTTCCAGAAGAGCCCACTCTTTGACCACAGCTGGGTCCCCCAACTTGGCTGCCGGGACGTCATCTCC
  CATCAGTGCTTGGAGCAGTAGTAGCTGGCACGGGCGGATCAAAGGCGGCATGAAGGGATTTCAGAGCTTC
  ATGGTTTCAGATAGCAACATGAGTTTTGTTGAATTTGTTGAGCTGTTCAAATCATTCAGTGTCAGGAGCC
  GCAAGGACCTGAAGGATCTGTTTGATGTCTATGCAGTGCCCTGCAACCGATCTGGCTCCGAGTCAGCCCC
  ACTCTACACCAACCTGACAATTGATGAAAACACCAGCGATCTTCAGCCTGACCTAGATCTGTTGACCAGA
  AATGTCTCGGATTTGGGGTTGTTCATTAAGAGTAAACAGCAGCTATCGGACAACCAGAGGCAGATATCTG
  ATGCCATTGCTGCTGCAAGCATTGTGACAAATGGCACTGGGATTGAGAGCACATCTCTGGGCATTTTTGG
  GGTGGGCATACTTCAGCTCAACGATTTCCTCGTGAATTGCCAAGGAGAACACTGCACTTATGATGAAATC
  CTCAGCATCATCCAGAAGTTCGAGCCTAGCATCAGTATGTGTCATCAGGGACTAATGTCATTTGAAGGGT
  TTGCCAGGTTTCTGATGGATAAAGAAAATTTTGCCTCAAAAAATGATGAGTCACAGGAGAACATTAAAGA
  ACTGCAGCTACCCCTCTCATACTATTACATCGAATCTTCGCACAATACCTACCTCACGGGCCATCAGCTC
  AAAGGAGAATCCTCGGTAGAACTCTACAGCCAGGTCCTTTTGCAAGGCTGTCGAAGTGTAGAATTGGACT
  GCTGGGACGGAGACGATGGGATGCCCATCATTTATCATGGACATACGCTGACAACCAAGATCCCCTTCAA
  GGAAGTGGTTGAAGCCATTGATCGCAGTGCCTTCATCAACTCTGACCTGCCAATCATCATATCGATTGAG
  AACCACTGTTCATTGCCTCAGCAACGAAAAATGGCAGAAATTTTCAAGACTGTGTTTGGAGAAAAGCTGG
  TGACTAAATTCTTATTTGAGACTGATTTCTCAGATGATCCAATGCTTCCTTCACCTGACCAACTCAGAAA
  GAAAGTTCTTCTTAAAAACAAGAAGCTAAAAGCCCATCAGACGCCAGTGGATATCTTAAAGCAAAAGGCT
  CATCAGTTAGCATCTATGCAAGTGCAGGCTTATAATGGTGGGAATGCCAACCCCCGACCTGCCAATAATG
  AGGAAGAGGAAGATGAGGAGGACGAATATGATTATGACTATGAATCCCTTTCTGATGACAACATTCTGGA
  AGACAGACCTGAAAATAAATCATGTAATGACAAGCTTCAGTTTGAATATAATGAAGAAATCCCAAAGAGG
  ATAAAGAAAGCAGATAACTCTGCTTGCAACAAAGGAAAGGTTTATGATATGGAACTGGGAGAAGAATTTT
  ATCTTGATCAGAATAAAAAGGAAAGCAGACAGATTGCACCAGAGCTTTCTGACCTTGTAATCTATTGTCA
  AGCAGTAAAATTTCCAGGACTGTCAACTCTAAATGCATCTGGCTCTAGCAGAGGAAAAGAAAGGAAAAGC
  AGGAAGTCCATTTTTGGCAACAATCCGGGCAGAATGAGCCCAGGGGAGACAGCATCATTTAACAAAACAT
  CTGGAAAAAGTTCCTGTGAAGGCATTCGACAGACCTGGGAGGAATCTTCTTCCCCTCTCAACCCAACCAC
  GTCCCTCAGTGCTATCATTAGAACTCCCAAATGTTATCATATCTCGTCGCTGAATGAAAATGCCGCCAAA
  CGTCTGTGTCGCAGGTATTCTCAGAAACTGACCCAGCACACCGCCTGTCAGCTGCTGAGAACTTACCCTG
  CTGCCACCCGCATCGACTCTTCCAACCCGAACCCCCTCATGTTCTGGCTCCATGGGATACAGCTTGTGGC
  ACTCAACTACCAGACTGATGATCTCCCTTTACATTTAAATGCTGCAATGTTTGAGGCAAATGGTGGTTGT
  GGTTATGTATTGAAACCTCCAGTTCTGTGGGACAAGAACTGCCCCATGTATCAGAAGTTTTCTCCACTAG
  AAAGAGATCTGGACAGCATGGATCCTGCAGTCTATTCTTTAACTATTGTCTCTGGTCAGAATGTGTGCCC
  CAGTAATAGCATGGGAAGCCCGTGCATTGAAGTCGACGTCCTGGGCATGCCTCTGGACAGCTGCCATTTC
  CGCACAAAGCCCATCCATCGAAACACCCTGAACCCCATGTGGAACGAGCAGTTTCTGTTCCACGTTCACT
  TCGAAGATCTTGTATTTCTTCGTTTTGCAGTTGTGGAAAACAATAGTTCAGCGGTAACTGCTCAGAGAAT
  CATTCCACTGAAAGCTTTAAAACGAGGATATCGACATCTTCAGCTGCGAAACCTTCACAATGAAGTCTTG
  GAGATTTCTAGTTTATTCATTAACAGCAGAAGGATGGAAGAAAATTCCTCTGGCAATACCATGTCAGCCT
  CTTCGATGTTTAATACAGAAGAAAGAAAATGTTTGCAGACTCACAGAGTCACGGTGCATGGGGTCCCAGG
  GCCAGAGCCCTTTACCGTTTTCACTATTAATGGAGGCACCAAGGCAAAGCAGCTTCTGCAGCAAATTCTG
  ACAAATGAACAAGACATCAAACCTGTTACCACAGACTATTTTTTGATGGAAGAAAAATATTTTATATCTA
  AAGAAAAGAATGAATGTAGGAAACAACCATTCCAGAGAGCCATTGGTCCAGAAGAGGAGATCATGCAAAT
  TTTAAGCAGCTGGTTTCCAGAAGAGGGATACATGGGCAGGATTGTCTTAAAAACCCAGCAGGAAAACCTA
  GAAGAGAAAAACATTGTTCAAGATGACAAAGAGGTGATCTTGAGCTCAGAGGAGGAGAGTTTCTTTGTCC
  AAGTGCATGATGTTTCTCCAGAGCAACCTCGAACAGTCATCAAAGCACCCCGCGTCAGCACTGCACAGGA
  TGTCATTCAGCAGACCTTATGCAAAGCCAAATATTCCTACAGCATCCTGAGCAACCCCAATCCAAGCGAC
  TATGTGCTTTTGGAAGAGGTGGTGAAAGACACTACCAACAAGAAGACTACCACACCAAAGTCCTCTCAGC
  GGGTCCTTCTGGATCAGGAGTGTGTGTTTCAAGCCCAAAGCAAGTGGAAAGGTGCAGGAAAATTCATCCT
  TAAGCTAAAGGAGCAGGTGCAGGCATCTCGAGAAGATAAAAAGAAAGGCATTTCTTTCGCAAGTGAACTC
  AAGAAGCTCACCAAGTCAACTAAACAGCCCCGAGGACTTACATCACCTTCTCAGCTCTTGACCTCAGAAA
  GTATCCAAACCAAGGAGGAGAAACCTGTGGGTGGCTTGTCCTCCAGTGACACAATGGATTACCGACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC240241 representing NM_001288989
  Red=Cloning site Green=Tags(s)
  
  MTSEEMTASVLIPVTQRKVVSAQSAADESSEKVSDINISKAHTVRRSGETSHTISQLNKLKEEPSGSNLP
  KILSIAREKIVSDENSNEKCWEKIMPDSAKNLNINCNNILRNHQHGLPQRQFYEMYNSVAEEDLCLETGI
  PSPLERKVFPGIQLELDRPSMGISPLGNQSVIIETGRAHPDSRRAVFHFHYEVDRRMSDTFCTLSENLIL
  DDCGNCVPLPGGEEKQKKNYVAYTCKLMELAKNCDNKNEQLQCDHCDTLNDKYFCFEGSCEKVDMVYSGD
  SFCRKDFTDSQAAKTFLSHFEDFPDNCDDVEEDAFKSKKERSTLLVRRFCKNDREVKKSVYTGTRAIVRT
  LPSGHIGLTAWSYIDQKRNGPLLPCGRVMEPPSTVEIRQDGSQRLSEAQWYPIYNAVRREETENTVGSLL
  HFLTKLPASETAHGRISVGPCLKQCVRDTVCEYRATLQRTSISQYITGSLLEATTSLGARSGLLSTFGGS
  TGRMMLKERQPGPSVANSNALPSSSAGISKELIDLQPLIQFPEEVASILMEQEQTIYRRVLPVDYLCFLT
  RDLGTPECQSSLPCLKASISASILTTQNGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVH
  VAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSCEYRKVVTRALHIPGC
  KVVPFCGVFLKELCEVLDGASGLMKLCPRYNSQEETLEFVADYSGQDNFLQRVGQNGLKNSEKESTVNSI
  FQVIRSCNRSLETDEEDSPSEGNSSRKSSLKDKSRWQFIIGDLLDSDNDIFEQSKEYDSHGSEDSQKAFD
  HGTELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWVKPTTASPASSKAKLGV
  LNNTAEPGKFPLLGNAGLSSLTEGVLDLFAVKAVYMGHPGIDIHTVCVQNKLGSMFLSETGVTLLYGLQT
  TDNRLLHFVAPKHTAKMLFSGLLELTRAVRKMRKFPDQRQQWLRKQYVSLYQEDGRYEGPTLAHAVELFG
  GRRWSARNPSPGTSAKNAEKPNMQRNNTLGISTTKKKKKILMRGESGEVTDDEMATRKAKMHKECRSRSG
  SDPQDINEQEESEVNAIANPPNPLPSRRAHSLTTAGSPNLAAGTSSPISAWSSSSWHGRIKGGMKGFQSF
  MVSDSNMSFVEFVELFKSFSVRSRKDLKDLFDVYAVPCNRSGSESAPLYTNLTIDENTSDLQPDLDLLTR
  NVSDLGLFIKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEI
  LSIIQKFEPSISMCHQGLMSFEGFARFLMDKENFASKNDESQENIKELQLPLSYYYIESSHNTYLTGHQL
  KGESSVELYSQVLLQGCRSVELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFINSDLPIIISIE
  NHCSLPQQRKMAEIFKTVFGEKLVTKFLFETDFSDDPMLPSPDQLRKKVLLKNKKLKAHQTPVDILKQKA
  HQLASMQVQAYNGGNANPRPANNEEEEDEEDEYDYDYESLSDDNILEDRPENKSCNDKLQFEYNEEIPKR
  IKKADNSACNKGKVYDMELGEEFYLDQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNASGSSRGKERKS
  RKSIFGNNPGRMSPGETASFNKTSGKSSCEGIRQTWEESSSPLNPTTSLSAIIRTPKCYHISSLNENAAK
  RLCRRYSQKLTQHTACQLLRTYPAATRIDSSNPNPLMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGC
  GYVLKPPVLWDKNCPMYQKFSPLERDLDSMDPAVYSLTIVSGQNVCPSNSMGSPCIEVDVLGMPLDSCHF
  RTKPIHRNTLNPMWNEQFLFHVHFEDLVFLRFAVVENNSSAVTAQRIIPLKALKRGYRHLQLRNLHNEVL
  EISSLFINSRRMEENSSGNTMSASSMFNTEERKCLQTHRVTVHGVPGPEPFTVFTINGGTKAKQLLQQIL
  TNEQDIKPVTTDYFLMEEKYFISKEKNECRKQPFQRAIGPEEEIMQILSSWFPEEGYMGRIVLKTQQENL
  EEKNIVQDDKEVILSSEEESFFVQVHDVSPEQPRTVIKAPRVSTAQDVIQQTLCKAKYSYSILSNPNPSD
  YVLLEEVVKDTTNKKTTTPKSSQRVLLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKKKGISFASEL
  KKLTKSTKQPRGLTSPSQLLTSESIQTKEEKPVGGLSSSDTMDYRQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001288989
• ORF Size: 6858 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001288989.1, NP_001275918.1
• RefSeq Size: 7957
• RefSeq ORF: 6861
• Locus ID: 51196
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• MW: 257.6 kDa
• Gene Summary: This gene encodes a phospholipase enzyme that catalyzes the hydrolysis of phosphatidylinositol-4,5-bisphosphate to generate two second messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). These second messengers subsequently regulate various processes affecting cell growth, differentiation, and gene expression. This enzyme is regulated by small monomeric GTPases of the Ras and Rho families and by heterotrimeric G proteins. In addition to its phospholipase C catalytic activity, this enzyme has an N-terminal domain with guanine nucleotide exchange (GEF) activity. Mutations in this gene cause early-onset nephrotic syndrome; characterized by proteinuria, edema, and diffuse mesangial sclerosis or focal and segmental glomerulosclerosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2009]