TIE1 (NM_005424) Human Tagged ORF Clone

CAT#: RC600056

TIE1 (DDK-His-tagged)-Extra Cellular Domain Clone of Homo sapiens tyrosine kinase with immunoglobulin-like and EGF-like domains 1, transcript variant 1, Signal peptide (1-21) plus EC domain (22-759)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: DDK-His
• Symbol: TIE1
• Synonyms: JTK14; TIE
• Vector: pCMV6-XL5-DDK-His
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC600056 representing leader sequence plus the extracellular domain region of NM_005424
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GTAATACGACTCACTATAGGGCGGCCGCGAATTCGTCGACTGGATCTGGTACCGAGGAGATCCGCCGCCG
  CGATCGCC
  
  ATGGTCTGGCGGGTGCCCCCTTTCTTGCTCCCCATCCTCTTCTTGGCTTCTCATGTGGGCGCGGCGGTGG
  ACCTGACGCTGCTGGCCAACCTGCGGCTCACGGACCCCCAGCGCTTCTTCCTGACTTGCGTGTCTGGGGA
  GGCCGGGGCGGGGAGGGGCTCGGACGCCTGGGGCCCGCCCCTGCTGCTGGAGAAGGACGACCGTATCGTG
  CGCACCCCGCCCGGGCCACCCCTGCGCCTGGCGCGCAACGGTTCGCACCAGGTCACGCTTCGCGGCTTCT
  CCAAGCCCTCGGACCTCGTGGGCGTCTTCTCCTGCGTGGGCGGTGCTGGGGCGCGGCGCACGCGCGTCAT
  CTACGTGCACAACAGCCCTGGAGCCCACCTGCTTCCAGACAAGGTCACACACACTGTGAACAAAGGTGAC
  ACCGCTGTACTTTCTGCACGTGTGCACAAGGAGAAGCAGACAGACGTGATCTGGAAGAGCAACGGATCCT
  ACTTCTACACCCTGGACTGGCATGAAGCCCAGGATGGGCGGTTCCTGCTGCAGCTCCCAAATGTGCAGCC
  ACCATCGAGCGGCATCTACAGTGCCACTTACCTGGAAGCCAGCCCCCTGGGCAGCGCCTTCTTTCGGCTC
  ATCGTGCGGGGTTGTGGGGCTGGGCGCTGGGGGCCAGGCTGTACCAAGGAGTGCCCAGGTTGCCTACATG
  GAGGTGTCTGCCACGACCATGACGGCGAATGTGTATGCCCCCCTGGCTTCACTGGCACCCGCTGTGAACA
  GGCCTGCAGAGAGGGCCGTTTTGGGCAGAGCTGCCAGGAGCAGTGCCCAGGCATATCAGGCTGCCGGGGC
  CTCACCTTCTGCCTCCCAGACCCCTATGGCTGCTCTTGTGGATCTGGCTGGAGAGGAAGCCAGTGCCAAG
  AAGCTTGTGCCCCTGGTCATTTTGGGGCTGATTGCCGACTCCAGTGCCAGTGTCAGAATGGTGGCACTTG
  TGACCGGTTCAGTGGTTGTGTCTGCCCCTCTGGGTGGCATGGAGTGCACTGTGAGAAGTCAGACCGGATC
  CCCCAGATCCTCAACATGGCCTCAGAACTGGAGTTCAACTTAGAGACGATGCCCCGGATCAACTGTGCAG
  CTGCAGGGAACCCCTTCCCCGTGCGGGGCAGCATAGAGCTACGCAAGCCAGACGGCACTGTGCTCCTGTC
  CACCAAGGCCATTGTGGAGCCAGAGAAGACCACAGCTGAGTTCGAGGTGCCCCGCTTGGTTCTTGCGGAC
  AGTGGGTTCTGGGAGTGCCGTGTGTCCACATCTGGCGGCCAAGACAGCCGGCGCTTCAAGGTCAATGTGA
  AAGTGCCCCCCGTGCCCCTGGCTGCACCTCGGCTCCTGACCAAGCAGAGCCGCCAGCTTGTGGTCTCCCC
  GCTGGTCTCGTTCTCTGGGGATGGACCCATCTCCACTGTCCGCCTGCACTACCGGCCCCAGGACAGTACC
  ATGGACTGGTCGACCATTGTGGTGGACCCCAGTGAGAACGTGACGTTAATGAACCTGAGGCCAAAGACAG
  GATACAGTGTTCGTGTGCAGCTGAGCCGGCCAGGGGAAGGAGGAGAGGGGGCCTGGGGGCCTCCCACCCT
  CATGACCACAGACTGTCCTGAGCCTTTGTTGCAGCCGTGGTTGGAGGGCTGGCATGTGGAAGGCACTGAC
  CGGCTGCGAGTGAGCTGGTCCTTGCCCTTGGTGCCCGGGCCACTGGTGGGCGACGGTTTCCTGCTGCGCC
  TGTGGGACGGGACACGGGGGCAGGAGCGGCGGGAGAACGTCTCATCCCCCCAGGCCCGCACTGCCCTCCT
  GACGGGACTCACGCCTGGCACCCACTACCAGCTGGATGTGCAGCTCTACCACTGCACCCTCCTGGGCCCG
  GCCTCGCCCCCTGCACACGTGCTTCTGCCCCCCAGTGGGCCTCCAGCCCCCCGACACCTCCACGCCCAGG
  CCCTCTCAGACTCCGAGATCCAGCTGACATGGAAGCACCCGGAGGCTCTGCCTGGGCCAATATCCAAGTA
  CGTTGTGGAGGTGCAGGTGGCTGGGGGTGCAGGAGACCCACTGTGGATAGACGTGGACAGGCCTGAGGAG
  ACAAGCACCATCATCCGTGGCCTCAACGCCAGCACGCGCTACCTCTTCCGCATGCGGGCCAGCATTCAGG
  GGCTCGGGGACTGGAGCAACACAGTAGAAGAGTCCACCCTGGGCAACGGGCTGCAGGCTGAGGGCCCAGT
  CCAAGAGAGCCGGGCAGCTGAAGAGGGCCTGGATCAG
  
  ACGCGTTCAGGCGACTACAAGGATGACGACGATAAGGGATCTCATCATCACCATCACCATTAATGAGATC
  TGGTACCGATATCAAGCTTGTCGACTCTAGA
  Protein Sequence

  >RC600056 representing signal peptide plus the extracellular domain region of NM_005424
  Red=Cloning sites Green= DDK and 6XHIS Tags
  
  MVWRVPPFLLPILFLASHVGAAVDLTLLANLRLTDPQRFFLTCVSGEAGAGRGSDAWGPPLLLEKDDRIV
  RTPPGPPLRLARNGSHQVTLRGFSKPSDLVGVFSCVGGAGARRTRVIYVHNSPGAHLLPDKVTHTVNKGD
  TAVLSARVHKEKQTDVIWKSNGSYFYTLDWHEAQDGRFLLQLPNVQPPSSGIYSATYLEASPLGSAFFRL
  IVRGCGAGRWGPGCTKECPGCLHGGVCHDHDGECVCPPGFTGTRCEQACREGRFGQSCQEQCPGISGCRG
  LTFCLPDPYGCSCGSGWRGSQCQEACAPGHFGADCRLQCQCQNGGTCDRFSGCVCPSGWHGVHCEKSDRI
  PQILNMASELEFNLETMPRINCAAAGNPFPVRGSIELRKPDGTVLLSTKAIVEPEKTTAEFEVPRLVLAD
  SGFWECRVSTSGGQDSRRFKVNVKVPPVPLAAPRLLTKQSRQLVVSPLVSFSGDGPISTVRLHYRPQDST
  MDWSTIVVDPSENVTLMNLRPKTGYSVRVQLSRPGEGGEGAWGPPTLMTTDCPEPLLQPWLEGWHVEGTD
  RLRVSWSLPLVPGPLVGDGFLLRLWDGTRGQERRENVSSPQARTALLTGLTPGTHYQLDVQLYHCTLLGP
  ASPPAHVLLPPSGPPAPRHLHAQALSDSEIQLTWKHPEALPGPISKYVVEVQVAGGAGDPLWIDVDRPEE
  TSTIIRGLNASTRYLFRMRASIQGLGDWSNTVEESTLGNGLQAEGPVQESRAAEEGLDQ
  
  TRSGTRSGDYKDDDDKGSHHHHHH
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_005424
• ORF Size: 2277 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the extra cellular domain of the protein with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_005424.4, NP_005415.1
• RefSeq Size: 4000
• RefSeq ORF: 3417
• Locus ID: 7075
• Domains: pkinase, TyrKc, S_TKc, ig, IG, FN3, EGF, EGF
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• MW: 82.2 kDa
• Gene Summary: This gene encodes a member of the tyrosine protein kinase family. The encoded protein plays a critical role in angiogenesis and blood vessel stability by inhibiting angiopoietin 1 signaling through the endothelial receptor tyrosine kinase Tie2. Ectodomain cleavage of the encoded protein relieves inhibition of Tie2 and is mediated by multiple factors including vascular endothelial growth factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]