Bif (SH3GLB1) (NM_016009) Human Tagged ORF Clone

CAT#: RG200106

SH3GLB1 (GFP-tagged) - Human SH3-domain GRB2-like endophilin B1 (SH3GLB1), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SH3GLB1
• Synonyms: Bif-1; CGI-61; dJ612B15.2; PPP1R70
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200106 representing NM_016009
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATATCATGGACTTCAACGTGAAGAAGCTGGCGGCCGACGCAGGCACCTTCCTCAGTCGCGCCGTGC
  AGTTCACAGAAGAAAAGCTTGGCCAGGCTGAGAAGACAGAATTGGATGCTCACTTAGAGAACCTCCTTAG
  CAAAGCTGAATGTACCAAAATATGGACAGAAAAAATAATGAAACAAACTGAAGTGTTATTGCAGCCAAAT
  CCAAATGCCAGGATAGAAGAATTTGTTTATGAGAAACTGGATAGAAAAGCTCCAAGTCGTATAAACAACC
  CAGAACTTTTGGGACAATATATGATTGATGCAGGGACTGAGTTTGGCCCAGGAACAGCTTATGGTAATGC
  CCTTATTAAATGTGGAGAAACCCAAAAAAGAATTGGAACAGCAGACAGAGAACTGATTCAAACGTCAGCC
  TTAAATTTTCTTACTCCTTTAAGAAACTTTATAGAAGGAGATTACAAAACAATTGCTAAAGAAAGGAAAC
  TATTGCAAAATAAGAGACTGGATTTGGATGCTGCAAAAACGAGACTAAAAAAGGCAAAAGCTGCAGAAAC
  TAGAAATTCATCTGAACAGGAATTAAGAATAACTCAAAGTGAATTTGATCGTCAAGCAGAGATTACCAGA
  CTTCTGCTAGAGGGAATCAGCAGTACACATGCCCATCACCTTCGCTGTCTGAATGACTTTGTAGAAGCCC
  AGATGACTTACTATGCACAGTGTTACCAGTATATGTTGGACCTCCAGAAACAACTGGGAAGTTTTCCATC
  CAATTATCTTAGTAACAACAATCAGACTTCTGTGACACCTGTACCATCAGTTTTACCAAATGCGATTGGT
  TCTTCTGCCATGGCTTCAACAAGTGGCCTAGTAATCACCTCTCCTTCCAACCTCAGTGACCTTAAGGAGT
  GTAGTGGCAGCAGAAAGGCCAGGGTTCTCTATGATTATGATGCAGCAAACAGTACTGAATTATCACTTCT
  GGCAGATGAGGTGATCACTGTGTTCAGTGTTGTTGGAATGGATTCAGACTGGCTAATGGGGGAAAGGGGA
  AACCAGAAGGGCAAGGTGCCAATTACCTACTTAGAACTGCTCAAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200106 representing NM_016009
  Red=Cloning site Green=Tags(s)
  
  MNIMDFNVKKLAADAGTFLSRAVQFTEEKLGQAEKTELDAHLENLLSKAECTKIWTEKIMKQTEVLLQPN
  PNARIEEFVYEKLDRKAPSRINNPELLGQYMIDAGTEFGPGTAYGNALIKCGETQKRIGTADRELIQTSA
  LNFLTPLRNFIEGDYKTIAKERKLLQNKRLDLDAAKTRLKKAKAAETRNSSEQELRITQSEFDRQAEITR
  LLLEGISSTHAHHLRCLNDFVEAQMTYYAQCYQYMLDLQKQLGSFPSNYLSNNNQTSVTPVPSVLPNAIG
  SSAMASTSGLVITSPSNLSDLKECSGSRKARVLYDYDAANSTELSLLADEVITVFSVVGMDSDWLMGERG
  NQKGKVPITYLELLN
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016009
• ORF Size: 1095 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_016009.3, NP_057093.1
• RefSeq Size: 6373
• RefSeq ORF: 1098
• Locus ID: 51100
• Domains: SH3, BAR
• Protein Pathways: Endocytosis
• Gene Summary: This gene encodes a SRC homology 3 domain-containing protein. The encoded protein interacts with the proapoptotic member of the Bcl-2 family, Bcl-2-associated X protein (Bax) and may be involved in regulating apoptotic signaling pathways. This protein may also be involved in maintaining mitochondrial morphology. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]