Adracalin (AAAS) (NM_015665) Human Tagged ORF Clone

CAT#: RG200154

AAAS (GFP-tagged) - Human achalasia, adrenocortical insufficiency, alacrimia (AAAS), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: AAAS
• Synonyms: AAA; AAASb; ADRACALA; ADRACALIN; ALADIN; GL003
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200154 representing NM_015665
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCTCTCTGGGGTTGTTCCCTCCTCCACCGCCTCGGGGTCAAGTCACCCTATATGAGCACAATAACG
  AGCTGGTGACGGGCAGTAGCTATGAGAGCCCGCCCCCCGACTTCCGGGGCCAGTGGATCAATCTTCCTGT
  CCTACAACTGACAAAGGATCCCCTAAAGACCCCTGGAAGGCTGGACCATGGCACAAGAACTGCCTTCATC
  CATCACCGGGAGCAAGTGTGGAAGAGATGCATCAACATTTGGCGTGATGTGGGCCTTTTTGGGGTGCTAA
  ATGAAATTGCAAACTCAGAAGAAGAGGTGTTTGAGTGGGTGAAGACGGCATCCGGCTGGGCCCTGGCACT
  CTGTCGATGGGCCTCTTCCCTCCATGGGTCCCTGTTCCCCCATCTGTCTCTCAGGAGCGAAGATCTGATC
  GCTGAATTTGCCCAAGTCACAAATTGGTCCAGCTGCTGCTTGCGTGTCTTTGCATGGCACCCCCACACCA
  ACAAGTTTGCAGTGGCCCTGCTAGATGACTCAGTCCGTGTGTATAATGCCAGCAGCACCATAGTCCCCTC
  CCTGAAGCACCGGCTGCAGCGAAATGTGGCGTCTCTGGCCTGGAAGCCCCTTAGTGCCTCTGTCTTGGCT
  GTGGCCTGCCAGAGCTGCATTCTTATCTGGACCCTGGACCCTACCTCCTTGTCTACCCGACCCTCTTCTG
  GCTGTGCCCAAGTGCTGTCTCACCCTGGGCATACACCTGTTACCAGCTTGGCCTGGGCCCCCAGTGGGGG
  GCGGCTGCTCTCAGCTTCACCCGTGGATGCTGCTATCCGGGTATGGGATGTCTCAACAGAGACCTGTGTC
  CCCCTTCCCTGGTTTCGAGGAGGTGGGGTGACCAACCTGCTCTGGTCCCCAGACGGCAGCAAAATCCTGG
  CTACCACTCCTTCAGCTGTCTTTCGAGTCTGGGAGGCCCAGATGTGGACTTGTGAGAGGTGGCCTACTCT
  ATCAGGGCGCTGTCAGACTGGCTGCTGGAGCCCAGATGGCAGCCGACTGCTGTTCACTGTATTGGGAGAG
  CCACTGATTTACTCCCTGTCTTTTCCAGAACGTTGTGGTGAGGGAAAGGGGTGCGTTGGAGGTGCAAAGT
  CAGCAACGATTGTGGCAGATCTGTCTGAGACAACAATACAGACACCAGATGGTGAGGAGAGGCTTGGGGG
  AGAGGCTCACTCCATGGTCTGGGACCCCAGTGGGGAACGTCTGGCTGTGCTTATGAAAGGAAAGCCAAGG
  GTACAGGATGGTAAACCAGTCATCCTCCTTTTTCGCACTCGAAACAGCCCTGTGTTTGAGCTCCTTCCCT
  GTGGCATTATCCAGGGGGAGCCAGGAGCCCAGCCCCAGCTCATCACTTTCCATCCTTCCTTCAACAAAGG
  GGCCCTGCTCAGTGTGGGCTGGTCCACAGGCCGAATTGCCCACATCCCGCTGTACTTTGTCAATGCCCAG
  TTTCCACGTTTTAGCCCAGTGCTTGGGCGGGCCCAGGAACCCCCTGCTGGGGGTGGAGGCTCTATTCATG
  ACCTGCCCCTCTTTACTGAGACATCCCCAACCTCTGCCCCTTGGGACCCTCTCCCAGGGCCACCACCTGT
  TCTGCCCCACTCCCCACATTCCCACCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200154 representing NM_015665
  Red=Cloning site Green=Tags(s)
  
  MCSLGLFPPPPPRGQVTLYEHNNELVTGSSYESPPPDFRGQWINLPVLQLTKDPLKTPGRLDHGTRTAFI
  HHREQVWKRCINIWRDVGLFGVLNEIANSEEEVFEWVKTASGWALALCRWASSLHGSLFPHLSLRSEDLI
  AEFAQVTNWSSCCLRVFAWHPHTNKFAVALLDDSVRVYNASSTIVPSLKHRLQRNVASLAWKPLSASVLA
  VACQSCILIWTLDPTSLSTRPSSGCAQVLSHPGHTPVTSLAWAPSGGRLLSASPVDAAIRVWDVSTETCV
  PLPWFRGGGVTNLLWSPDGSKILATTPSAVFRVWEAQMWTCERWPTLSGRCQTGCWSPDGSRLLFTVLGE
  PLIYSLSFPERCGEGKGCVGGAKSATIVADLSETTIQTPDGEERLGGEAHSMVWDPSGERLAVLMKGKPR
  VQDGKPVILLFRTRNSPVFELLPCGIIQGEPGAQPQLITFHPSFNKGALLSVGWSTGRIAHIPLYFVNAQ
  FPRFSPVLGRAQEPPAGGGGSIHDLPLFTETSPTSAPWDPLPGPPPVLPHSPHSHL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015665
• ORF Size: 1638 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015665.3, NP_056480.1
• RefSeq Size: 1816
• RefSeq ORF: 1641
• Locus ID: 8086
• Domains: WD40
• Gene Summary: The protein encoded by this gene is a member of the WD-repeat family of regulatory proteins and may be involved in normal development of the peripheral and central nervous system. The encoded protein is part of the nuclear pore complex and is anchored there by NDC1. Defects in this gene are a cause of achalasia-addisonianism-alacrima syndrome (AAAS), also called triple-A syndrome or Allgrove syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]