PRPF4 (NM_004697) Human Tagged ORF Clone

CAT#: RG200360

PRPF4 (GFP-tagged) - Human PRP4 pre-mRNA processing factor 4 homolog (yeast) (PRPF4)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PRPF4
• Synonyms: HPRP4; HPRP4P; PRP4; Prp4p; RP70; SNRNP60
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200360 representing NM_004697
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCCTCGCGAGCCTCTTCCACGCAGGCAACCAAAACTAAAGCACCCGACGACTTAGTTGCTCCGG
  TCGTGAAGAAACCACACATCTATTATGGAAGTTTGGAAGAGAAGGAGAGGGAGCGTCTGGCCAAAGGAGA
  GTCTGGGATTTTGGGGAAAGACGGACTTAAAGCAGGGATCGAAGCTGGAAATATTAATATAACCTCTGGA
  GAAGTGTTTGAAATTGAAGAGCATATCAGCGAGCGACAGGCAGAAGTATTGGCTGAGTTTGAGAGAAGGA
  AGCGAGCCCGGCAGATCAATGTTTCCACAGATGACTCAGAGGTCAAAGCTTGCCTTAGAGCCTTGGGGGA
  ACCCATCACACTTTTTGGAGAGGGTCCTGCTGAAAGAAGAGAAAGGTTAAGAAATATCCTCTCAGTTGTC
  GGTACTGATGCCTTGAAAAAGACCAAAAAGGATGATGAGAAGTCTAAAAAGTCCAAAGAAGAGTATCAGC
  AAACCTGGTATCATGAAGGACCAAATAGCTTGAAGGTGGCAAGACTATGGATTGCTAATTATTCGTTGCC
  CAGGGCAATGAAACGCTTGGAAGAGGCCCGACTCCATAAGGAGATTCCTGAGACAACAAGGACCTCCCAG
  ATGCAAGAGCTGCACAAGTCTCTCCGGTCTTTGAATAATTTTTGCAGTCAGATTGGGGATGATCGGCCTA
  TCTCCTACTGTCACTTTAGTCCCAATTCCAAGATGCTGGCCACAGCTTGTTGGAGTGGGCTTTGCAAGCT
  CTGGTCTGTTCCTGATTGCAACCTCCTTCACACTCTTCGAGGGCATAACACAAATGTAGGAGCAATTGTA
  TTCCATCCCAAATCCACTGTCTCCTTGGACCCAAAAGATGTCAACCTGGCCTCTTGTGCGGCTGATGGCT
  CTGTGAAGCTTTGGAGTCTCGACAGTGATGAACCAGTGGCAGATATTGAAGGCCATACAGTGCGTGTGGC
  GCGGGTAATGTGGCATCCTTCAGGACGTTTCCTGGGCACCACCTGCTATGACCGTTCATGGCGCTTATGG
  GATTTGGAGGCTCAAGAGGAGATCCTGCATCAGGAAGGCCATAGCATGGGTGTGTATGACATTGCCTTCC
  ATCAAGATGGCTCTTTGGCTGGCACTGGGGGACTGGATGCATTTGGTCGAGTTTGGGACCTACGCACAGG
  ACGTTGTATCATGTTCTTAGAAGGCCACCTGAAAGAAATCTATGGAATAAATTTCTCCCCCAATGGCTAT
  CACATTGCAACCGGCAGTGGTGACAACACCTGCAAAGTGTGGGACCTCCGACAGCGGCGTTGCGTCTACA
  CCATCCCTGCTCATCAGAACTTAGTGACTGGTGTCAAGTTTGAGCCTATCCATGGGAACTTCTTGCTTAC
  TGGTGCCTATGATAACACAGCCAAGATCTGGACGCACCCAGGCTGGTCCCCGCTGAAGACTCTGGCTGGC
  CACGAAGGCAAAGTGATGGGCCTAGATATTTCTTCCGATGGGCAGCTCATAGCCACTTGCTCATATGACA
  GGACCTTCAAGCTGTGGATGGCTGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200360 representing NM_004697
  Red=Cloning site Green=Tags(s)
  
  MASSRASSTQATKTKAPDDLVAPVVKKPHIYYGSLEEKERERLAKGESGILGKDGLKAGIEAGNINITSG
  EVFEIEEHISERQAEVLAEFERRKRARQINVSTDDSEVKACLRALGEPITLFGEGPAERRERLRNILSVV
  GTDALKKTKKDDEKSKKSKEEYQQTWYHEGPNSLKVARLWIANYSLPRAMKRLEEARLHKEIPETTRTSQ
  MQELHKSLRSLNNFCSQIGDDRPISYCHFSPNSKMLATACWSGLCKLWSVPDCNLLHTLRGHNTNVGAIV
  FHPKSTVSLDPKDVNLASCAADGSVKLWSLDSDEPVADIEGHTVRVARVMWHPSGRFLGTTCYDRSWRLW
  DLEAQEEILHQEGHSMGVYDIAFHQDGSLAGTGGLDAFGRVWDLRTGRCIMFLEGHLKEIYGINFSPNGY
  HIATGSGDNTCKVWDLRQRRCVYTIPAHQNLVTGVKFEPIHGNFLLTGAYDNTAKIWTHPGWSPLKTLAG
  HEGKVMGLDISSDGQLIATCSYDRTFKLWMAE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004697
• ORF Size: 1566 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_004697.3, NP_004688.2
• RefSeq Size: 2756
• RefSeq ORF: 1569
• Locus ID: 9128
• Domains: WD40, SFM
• Protein Pathways: Spliceosome
• Gene Summary: The protein encoded by this gene is part of a heteromeric complex that binds U4, U5, and U6 small nuclear RNAs and is involved in pre-mRNA splicing. The encoded protein also is a mitotic checkpoint protein and a regulator of chemoresistance in human ovarian cancer. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2016]