NDUFS3 (NM_004551) Human Tagged ORF Clone

CAT#: RG200567

NDUFS3 (GFP-tagged) - Human NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Q reductase) (NDUFS3), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NDUFS3
• Synonyms: CI-30; MC1DN8
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200567 representing NM_004551
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCGGCGGTAGCCAGGCTGTGGTGGCGCGGGATCTTGGGGGCCTCGGCGCTGACCAGGGGGA
  CTGGGCGACCCTCCGTTCTGTTGCTGCCGGTGAGGCGGGAGAGCGCCGGGGCCGACACGCGCCCCACTGT
  CAGACCACGGAATGATGTGGCCCACAAGCAGCTCTCAGCTTTTGGAGAGTATGTGGCTGAAATCTTGCCC
  AAGTATGTCCAACAAGTTCAGGTGTCCTGCTTCAATGAGTTAGAGGTCTGTATCCATCCTGATGGCGTCA
  TCCCAGTGCTGACTTTCCTCAGGGATCACACCAATGCACAGTTCAAATCTCTGGTTGACTTGACAGCAGT
  GGACGTCCCAACTCGGCAAAACCGTTTTGAGATTGTCTACAACCTGTTGTCTCTGCGCTTCAACTCACGG
  ATCCGTGTGAAGACCTACACAGATGAGCTGACGCCCATTGAGTCTGCTGTCTCTGTGTTCAAGGCAGCCA
  ACTGGTATGAAAGGGAGATCTGGGACATGTTTGGAGTCTTCTTTGCTAACCACCCTGATCTAAGAAGGAT
  CCTGACAGATTATGGCTTCGAGGGACATCCTTTCCGGAAAGACTTTCCTCTATCTGGCTATGTTGAGTTA
  CGTTATGATGATGAAGTGAAGCGGGTGGTGGCAGAGCCGGTGGAGTTGGCCCAAGAGTTCCGCAAATTTG
  ACCTGAACAGCCCCTGGGAGGCTTTCCCAGTCTATCGCCAACCCCCGGAGAGTCTCAAGCTTGAAGCCGG
  AGACAAGAAGCCTGATGCCAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200567 representing NM_004551
  Red=Cloning site Green=Tags(s)
  
  MAAAAVARLWWRGILGASALTRGTGRPSVLLLPVRRESAGADTRPTVRPRNDVAHKQLSAFGEYVAEILP
  KYVQQVQVSCFNELEVCIHPDGVIPVLTFLRDHTNAQFKSLVDLTAVDVPTRQNRFEIVYNLLSLRFNSR
  IRVKTYTDELTPIESAVSVFKAANWYEREIWDMFGVFFANHPDLRRILTDYGFEGHPFRKDFPLSGYVEL
  RYDDEVKRVVAEPVELAQEFRKFDLNSPWEAFPVYRQPPESLKLEAGDKKPDAK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004551
• ORF Size: 792 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004551.1, NP_004542.1
• RefSeq Size: 899 bp
• RefSeq ORF: 795 bp
• Locus ID: 4722
• Cytogenetics: 11p11.2
• Domains: complex1_30Kd
• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• Gene Summary: 'This gene encodes one of the iron-sulfur protein (IP) components of mitochondrial NADH:ubiquinone oxidoreductase (complex I). Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency.[provided by RefSeq, Apr 2009]'