NCBP1 (NM_002486) Human Tagged ORF Clone

CAT#: RG200630

NCBP1 (GFP-tagged) - Human nuclear cap binding protein subunit 1, 80kDa (NCBP1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NCBP1
• Synonyms: CBP80; NCBP; Sto1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200630 representing NM_002486
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCGGCGGCGGCACAGCGACGAGAACGACGGTGGGCAGCCTCACAAAAGGAGAAAGACCTCTGATG
  CAAATGAAACTGAAGATCATTTGGAATCTTTAATATGTAAAGTAGGAGAAAAGAGTGCCTGCTCTTTGGA
  GAGCAACCTAGAAGGCTTGGCTGGTGTTTTGGAAGCTGATCTTCCTAACTACAAGAGCAAGATCTTAAGG
  CTTCTTTGTACAGTTGCACGCCTATTACCTGAGAAGCTGACAATTTATACAACATTAGTTGGACTACTGA
  ATGCCAGGAATTACAATTTTGGTGGAGAATTTGTAGAAGCCATGATTCGTCAACTTAAAGAATCATTGAA
  AGCAAACAATTATAATGAAGCCGTGTATTTGGTCCGTTTTTTATCTGATCTTGTGAATTGTCATGTGATT
  GCCGCCCCATCAATGGTTGCTATGTTTGAAAATTTTGTAAGCGTAACTCAGGAAGAAGATGTACCTCAGG
  TGCGACGAGATTGGTATGTGTATGCATTTCTGTCATCTTTGCCCTGGGTTGGAAAGGAGTTGTACGAAAA
  GAAAGATGCAGAGATGGACCGCATCTTTGCCAACACTGAAAGCTATCTTAAAAGACGCCAAAAGACTCAT
  GTACCCATGTTACAGGTATGGACTGCTGATAAACCACATCCACAAGAAGAGTATTTAGATTGCCTGTGGG
  CCCAGATTCAGAAATTGAAAAAGGATCGCTGGCAGGAACGGCACATCCTAAGACCTTATCTTGCCTTTGA
  CAGCATCCTGTGTGAAGCACTGCAGCACAATCTGCCTCCTTTTACACCACCTCCTCACACTGAAGATTCA
  GTGTACCCAATGCCAAGGGTCATCTTCAGAATGTTTGATTACACAGATGATCCCGAGGGTCCTGTCATGC
  CAGGGAGTCATTCAGTGGAAAGATTTGTAATAGAAGAGAATCTTCACTGCATCATTAAGTCCCACTGGAA
  GGAAAGGAAGACTTGTGCTGCACAGTTAGTGAGCTATCCAGGGAAGAACAAGATCCCCTTGAACTACCAC
  ATAGTTGAGGTGATCTTTGCAGAGCTGTTTCAACTTCCAGCACCCCCTCACATTGATGTGATGTACACAA
  CACTCCTCATTGAACTGTGCAAACTTCAACCTGGCTCTCTACCCCAAGTTCTTGCACAGGCAACTGAAAT
  GCTATACATGCGTTTGGACACAATGAACACTACCTGTGTAGACAGGTTTATTAATTGGTTTTCTCATCAT
  CTAAGTAACTTCCAGTTCCGTTGGAGCTGGGAAGATTGGTCAGATTGTCTTAGTCAAGATCCTGAAAGTC
  CCAAACCGAAGTTTGTAAGAGAAGTTCTAGAAAAATGTATGAGGTTGTCTTACCATCAGCGTATATTAGA
  TATTGTTCCTCCTACCTTCTCAGCTCTGTGTCCTGCAAACCCAACCTGCATTTACAAGTATGGAGATGAA
  AGTAGCAATTCTCTTCCTGGACATTCTGTTGCCCTCTGTTTAGCTGTTGCCTTTAAAAGTAAGGCAACCA
  ATGATGAAATCTTCAGCATTCTGAAAGATGTACCAAATCCTAACCAGGATGATGACGACGATGAAGGATT
  CAGTTTTAACCCATTGAAAATAGAAGTCTTTGTACAGACTCTGCTACACTTGGCAGCCAAATCATTCAGC
  CACTCCTTCAGTGCTCTTGCAAAGTTTCATGAAGTCTTCAAAACCCTAGCTGAAAGTGATGAAGGAAAGT
  TACATGTGCTAAGAGTTATGTTTGAGGTCTGGAGGAACCATCCACAGATGATTGCTGTACTAGTGGATAA
  GATGATTCGTACACAAATAGTTGATTGTGCTGCCGTAGCAAATTGGATCTTCTCTTCAGAACTATCTCGT
  GACTTTACCAGATTGTTTGTTTGGGAAATTTTGCACTCTACAATTCGTAAGATGAACAAACATGTCCTGA
  AGATCCAGAAAGAGCTGGAAGAAGCTAAAGAGAAACTTGCTAGGCAACACAAACGGCGAAGTGATGATGA
  CGACAGAAGCAGTGACAGGAAAGACGGGGTTCTTGAGGAACAAATAGAACGACTTCAGGAAAAAGTGGAA
  TCTGCTCAGAGTGAACAAAAGAATCTTTTCCTCGTTATATTTCAGCGGTTTATCATGATCTTGACCGAGC
  ACCTAGTACGATGCGAAACTGATGGGACCAGTGTATTAACACCATGGTATAAGAACTGTATAGAGAGGCT
  GCAGCAGATCTTCCTACAGCATCACCAAATAATCCAGCAGTACATGGTGACCCTGGAGAACCTTCTCTTC
  ACTGCTGAATTAGACCCTCATATCTTGGCCGTGTTCCAGCAGTTCTGTGCCCTGCAGGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200630 representing NM_002486
  Red=Cloning site Green=Tags(s)
  
  MSRRRHSDENDGGQPHKRRKTSDANETEDHLESLICKVGEKSACSLESNLEGLAGVLEADLPNYKSKILR
  LLCTVARLLPEKLTIYTTLVGLLNARNYNFGGEFVEAMIRQLKESLKANNYNEAVYLVRFLSDLVNCHVI
  AAPSMVAMFENFVSVTQEEDVPQVRRDWYVYAFLSSLPWVGKELYEKKDAEMDRIFANTESYLKRRQKTH
  VPMLQVWTADKPHPQEEYLDCLWAQIQKLKKDRWQERHILRPYLAFDSILCEALQHNLPPFTPPPHTEDS
  VYPMPRVIFRMFDYTDDPEGPVMPGSHSVERFVIEENLHCIIKSHWKERKTCAAQLVSYPGKNKIPLNYH
  IVEVIFAELFQLPAPPHIDVMYTTLLIELCKLQPGSLPQVLAQATEMLYMRLDTMNTTCVDRFINWFSHH
  LSNFQFRWSWEDWSDCLSQDPESPKPKFVREVLEKCMRLSYHQRILDIVPPTFSALCPANPTCIYKYGDE
  SSNSLPGHSVALCLAVAFKSKATNDEIFSILKDVPNPNQDDDDDEGFSFNPLKIEVFVQTLLHLAAKSFS
  HSFSALAKFHEVFKTLAESDEGKLHVLRVMFEVWRNHPQMIAVLVDKMIRTQIVDCAAVANWIFSSELSR
  DFTRLFVWEILHSTIRKMNKHVLKIQKELEEAKEKLARQHKRRSDDDDRSSDRKDGVLEEQIERLQEKVE
  SAQSEQKNLFLVIFQRFIMILTEHLVRCETDGTSVLTPWYKNCIERLQQIFLQHHQIIQQYMVTLENLLF
  TAELDPHILAVFQQFCALQA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002486
• ORF Size: 2370 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002486.4, NP_002477.1
• RefSeq Size: 5381 bp
• RefSeq ORF: 2373 bp
• Locus ID: 4686
• Cytogenetics: 9q22.33
• Domains: MIF4G
• Protein Pathways: Spliceosome
• Gene Summary: 'The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein promotes high-affinity mRNA-cap binding and associates with the CTD of RNA polymerase II. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay. [provided by RefSeq, Jul 2008]'