PTPN7 (NM_002832) Human Tagged ORF Clone

CAT#: RG200699

PTPN7 (GFP-tagged) - Human protein tyrosine phosphatase, non-receptor type 7 (PTPN7), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PTPN7
• Synonyms: BPTP-4; HEPTP; LC-PTP; LPTP; PTPNI
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200699 representing NM_002832
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGCCTCCTTCTGGCCAATCAGGCAGGCCAGAGAACAGCAGCGGAGGGCCCTGTCTTTCAGGCAGA
  CCTCATGGCTGAGTGAGCCTCCCCTGGGCCCAGCACCCCACCTCAGCATGGTCCAAGCCCATGGGGGGCG
  CTCCAGAGCACAGCCGTTGACCTTGTCTTTGGGGGCAGCCATGACCCAGCCTCCGCCTGAAAAAACGCCA
  GCCAAGAAGCATGTGCGACTGCAGGAGAGGCGGGGCTCCAATGTGGCTCTGATGCTGGACGTTCGGTCCC
  TGGGGGCCGTAGAACCCATCTGCTCTGTGAACACACCCCGGGAGGTCACCCTACACTTTCTGCGCACTGC
  TGGACACCCCCTTACCCGCTGGGCCCTTCAGCGCCAGCCACCCAGCCCCAAGCAACTGGAAGAAGAATTC
  TTGAAGATCCCTTCAAACTTTGTCAGCCCCGAAGACCTGGACATCCCTGGCCACGCCTCCAAGGACCGAT
  ACAAGACCATCTTGCCAAATCCCCAGAGCCGTGTCTGTCTAGGCCGGGCACAGAGCCAGGAGGACGGAGA
  TTACATCAATGCCAACTACATCCGAGGCTATGACGGGAAGGAGAAGGTCTACATTGCCACCCAGGGCCCC
  ATGCCCAACACTGTGTCGGACTTCTGGGAGATGGTGTGGCAAGAGGAAGTGTCCCTCATTGTCATGCTCA
  CTCAGCTCCGAGAGGGCAAGGAGAAATGTGTCCACTACTGGCCCACAGAAGAGGAAACCTATGGACCCTT
  CCAGATCCGCATCCAGGACATGAAAGAGTGCCCAGAATACACTGTGCGGCAGCTCACCATCCAGTACCAG
  GAAGAGCGCCGGTCAGTAAAGCACATCCTCTTTTCGGCCTGGCCAGACCATCAGACACCAGAATCAGCTG
  GGCCCCTGCTGCGCCTAGTGGCAGAGGTGGAGGAGAGCCCGGAGACAGCCGCCCACCCCGGGCCTATCGT
  AGTCCACTGCAGTGCAGGGATTGGCCGGACGGGCTGCTTCATCGCCACGCGAATTGGCTGTCAACAGCTG
  AAAGCCCGAGGAGAAGTGGACATTCTGGGTATTGTGTGCCAACTGCGGCTAGACAGAGGGGGGATGATCC
  AGACGGCAGAGCAGTACCAGTTCCTGCACCACACTTTGGCCCTGTATGCAGGCCAGCTGCCTGAGGAACC
  CAGCCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200699 representing NM_002832
  Red=Cloning site Green=Tags(s)
  
  MGASFWPIRQAREQQRRALSFRQTSWLSEPPLGPAPHLSMVQAHGGRSRAQPLTLSLGAAMTQPPPEKTP
  AKKHVRLQERRGSNVALMLDVRSLGAVEPICSVNTPREVTLHFLRTAGHPLTRWALQRQPPSPKQLEEEF
  LKIPSNFVSPEDLDIPGHASKDRYKTILPNPQSRVCLGRAQSQEDGDYINANYIRGYDGKEKVYIATQGP
  MPNTVSDFWEMVWQEEVSLIVMLTQLREGKEKCVHYWPTEEETYGPFQIRIQDMKECPEYTVRQLTIQYQ
  EERRSVKHILFSAWPDHQTPESAGPLLRLVAEVEESPETAAHPGPIVVHCSAGIGRTGCFIATRIGCQQL
  KARGEVDILGIVCQLRLDRGGMIQTAEQYQFLHHTLALYAGQLPEEPSP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002832
• ORF Size: 1200 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_002832.2, NP_002823.2
• RefSeq Size: 3772
• RefSeq ORF: 1398
• Locus ID: 5778
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: MAPK signaling pathway
• Gene Summary: The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This gene is preferentially expressed in a variety of hematopoietic cells, and is an early response gene in lymphokine stimulated cells. The non-catalytic N-terminus of this PTP can interact with MAP kinases and suppress the MAP kinase activities. This PTP was shown to be involved in the regulation of T cell antigen receptor (TCR) signaling, which was thought to function through dephosphorylating the molecules related to MAP kinase pathway. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2010]