KPC2 (UBAC1) (NM_016172) Human Tagged ORF Clone

CAT#: RG200734

UBAC1 (GFP-tagged) - Human UBA domain containing 1 (UBAC1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: UBAC1
• Synonyms: GBDR1; KPC2; UBADC1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200734 representing NM_016172
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCGTGCAGGAGGAGAAGATCTTCGCGGGCAAGGTGCTGCGGCTGCACATCTGCGCGTCCGACGGCG
  CCGAGTGGCTGGAGGAGGCCACCGAGGACACCTCGGTGGAGAAGCTCAAGGAGCGCTGCCTCAAGCACTG
  TGCTCATGGGAGCTTAGAAGATCCCAAAAGTATAACCCATCATAAATTAATCCACGCTGCCTCAGAGAGG
  GTGCTGAGTGATGCCAGGACCATCCTGGAAGAGAACATCCAGGACCAAGATGTCCTATTATTGATAAAAA
  AGCGTGCTCCATCACCACTTCCCAAGATGGCTGATGTCTCAGCAGAAGAAAAGAAAAAACAAGACCAGAA
  AGCTCCAGATAAAGAGGCCATACTGCGGGCCACCGCCAACCTGCCCTCCTACAACATGGACCGGGCCGCG
  GTCCAGACCAACATGAGAGACTTCCAGACAGAACTCCGGAAGATACTGGTGTCTCTCATCGAGGTGGCGC
  AGAAGCTGTTAGCGCTGAACCCAGATGCGGTGGAATTGTTTAAGAAGGCGAATGCAATGCTGGACGAGGA
  CGAGGATGAGCGTGTGGACGAGGCTGCCCTGCGGCAGCTCACGGAGATGGGCTTTCCGGAGAACAGAGCC
  ACCAAGGCCCTTCAGCTGAACCACATGTCGGTGCCTCAGGCCATGGAGTGGCTAATTGAACACGCAGAAG
  ACCCGACCATAGACACGCCTCTTCCTGGCCAAGCTCCCCCAGAGGCCGAGGGGGCCACAGCAGCTGCCTC
  CGAGGCTGCCGCGGGAGCCAGCGCCACCGATGAGGAGGCCAGAGATGAGCTGACGGAAATCTTCAAGAAG
  ATCCGGAGGAAAAGGGAGTTTCGGGCTGATGCTCGGGCCGTCATTTCCCTGATGGAGATGGGGTTCGACG
  AGAAAGAGGTGATAGATGCCCTCAGAGTGAACAACAACCAGCAGAATGCCGCGTGCGAGTGGCTGCTGGG
  GGACCGGAAGCCCTCTCCGGAGGAGCTGGACAAGGGCATCGACCCCGACAGTCCTCTCTTTCAGGCCATC
  CTGGATAACCCGGTGGTGCAGCTGGGCCTGACCAACCCGAAAACATTGCTAGCATTTGAAGACATGCTGG
  AGAACCCACTGAACAGCACCCAGTGGATGAATGATCCAGAAACGGGGCCTGTCATGCTGCAGATCTCTAG
  AATCTTCCAGACACTAAATCGCACG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200734 representing NM_016172
  Red=Cloning site Green=Tags(s)
  
  MFVQEEKIFAGKVLRLHICASDGAEWLEEATEDTSVEKLKERCLKHCAHGSLEDPKSITHHKLIHAASER
  VLSDARTILEENIQDQDVLLLIKKRAPSPLPKMADVSAEEKKKQDQKAPDKEAILRATANLPSYNMDRAA
  VQTNMRDFQTELRKILVSLIEVAQKLLALNPDAVELFKKANAMLDEDEDERVDEAALRQLTEMGFPENRA
  TKALQLNHMSVPQAMEWLIEHAEDPTIDTPLPGQAPPEAEGATAAASEAAAGASATDEEARDELTEIFKK
  IRRKREFRADARAVISLMEMGFDEKEVIDALRVNNNQQNAACEWLLGDRKPSPEELDKGIDPDSPLFQAI
  LDNPVVQLGLTNPKTLLAFEDMLENPLNSTQWMNDPETGPVMLQISRIFQTLNRT
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016172
• ORF Size: 1215 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_016172.2, NP_057256.2
• RefSeq Size: 1877
• RefSeq ORF: 1218
• Locus ID: 10422
• Domains: UBA, STI1
• Protein Families: Druggable Genome
• Gene Summary: Non-catalytic subunit of the KPC complex that acts as E3 ubiquitin-protein ligase. Required for poly-ubiquitination and proteasome-mediated degradation of CDKN1B during G1 phase of the cell cycle. [UniProtKB/Swiss-Prot Function]