DCI (ECI1) (NM_001919) Human Tagged ORF Clone
CAT#: RG200871
- TrueORF®
ECI1 (GFP-tagged) - Human enoyl-CoA delta isomerase 1 (ECI1), nuclear gene encoding mitochondrial protein, transcript variant 1
"NM_001919" in other vectors (5)
Product Images
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | ECI1 |
Synonyms | DCI |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG200871 representing NM_001919
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCTGGTGGCTTCTGTGCGAGTCCCGGCGCGCGTTCTGCTCCGCGCGGGGGCCCGGCTCCCGGGCG CGGCCCTCGGGCGGACGGAGCGGGCGGCCGGCGGCGGAGACGGCGCGCGGCGCTTCGGGAGCCAGCGGGT GCTGGTGGAGCCGGACGCGGGCGCAGGGGTCGCTGTGATGAAATTCAAGAACCCCCCAGTGAACAGCCTG AGCCTGGAGTTTCTGACGGAGCTGGTCATCAGCCTGGAGAAGCTGGAGAATGACAAGAGCTTCCGCGGTG TCATTCTGACCTCGGACCGCCCGGGTGTCTTCTCGGCCGGCCTGGACCTGACGGAGATGTGTGGGAGGAG CCCCGCCCACTACGCTGGGTACTGGAAGGCCGTTCAGGAGCTGTGGCTGCGGTTGTACCAGTCCAACCTG GTGCTGGTCTCCGCCATCAACGGAGCCTGCCCCGCTGGAGGCTGCCTGGTGGCCCTGACCTGTGACTACC GCATCCTGGCGGACAACCCCAGGTACTGCATAGGACTCAATGAGACCCAGCTGGGCATCATCGCCCCTTT CTGGTTGAAAGACACCCTGGAGAACACCATCGGGCACCGGGCGGCGGAGCGTGCCCTGCAGCTGGGGCTG CTCTTCCCGCCGGCGGAGGCCCTGCAGGTGGGCATAGTGGACCAGGTGGTCCCGGAGGAGCAGGTGCAGA GCACTGCGCTGTCAGCGATAGCCCAGTGGATGGCCATTCCAGACCATGCTCGACAGCTGACCAAGGCCAT GATGCGAAAGGCCACGGCCAGCCGCCTGGTCACGCAGCGCGATGCGGACGTGCAGAACTTCGTCAGCTTC ATCTCCAAAGACTCCATCCAGAAGTCCCTGCAGATGTACTTAGAGAGGCTCAAAGAAGAAAAAGGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG200871 representing NM_001919
Red=Cloning site Green=Tags(s) MALVASVRVPARVLLRAGARLPGAALGRTERAAGGGDGARRFGSQRVLVEPDAGAGVAVMKFKNPPVNSL SLEFLTELVISLEKLENDKSFRGVILTSDRPGVFSAGLDLTEMCGRSPAHYAGYWKAVQELWLRLYQSNL VLVSAINGACPAGGCLVALTCDYRILADNPRYCIGLNETQLGIIAPFWLKDTLENTIGHRAAERALQLGL LFPPAEALQVGIVDQVVPEEQVQSTALSAIAQWMAIPDHARQLTKAMMRKATASRLVTQRDADVQNFVSF ISKDSIQKSLQMYLERLKEEKG TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001919 |
ORF Size | 906 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001919.3, NP_001910.2 |
RefSeq Size | 1069 bp |
RefSeq ORF | 909 bp |
Locus ID | 1632 |
Cytogenetics | 16p13.3 |
Domains | ECH |
Protein Pathways | Fatty acid metabolism |
Gene Summary | 'This gene encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2010]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC118944 | ECI1 (untagged)-Human enoyl-CoA delta isomerase 1 (ECI1), nuclear gene encoding mitochondrial protein, transcript variant 1 |
USD 310.00 |
|
SC320599 | ECI1 (untagged)-Human enoyl-CoA delta isomerase 1 (ECI1), nuclear gene encoding mitochondrial protein, transcript variant 1 |
USD 310.00 |
|
RC200871 | ECI1 (Myc-DDK-tagged)-Human enoyl-CoA delta isomerase 1 (ECI1), nuclear gene encoding mitochondrial protein, transcript variant 1 |
USD 420.00 |
|
RC200871L3 | Lenti-ORF clone of ECI1 (Myc-DDK-tagged)-Human enoyl-CoA delta isomerase 1 (ECI1), nuclear gene encoding mitochondrial protein, transcript variant 1 |
USD 620.00 |
|
RC200871L4 | Lenti-ORF clone of ECI1 (mGFP-tagged)-Human enoyl-CoA delta isomerase 1 (ECI1), nuclear gene encoding mitochondrial protein, transcript variant 1 |
USD 620.00 |
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