PDXK (NM_003681) Human Tagged ORF Clone

CAT#: RG200975

PDXK (GFP-tagged) - Human pyridoxal (pyridoxine, vitamin B6) kinase (PDXK)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PDXK
• Synonyms: C21orf97; C21orf124; HEL-S-1a; PKH; PNK; PRED79
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200975 representing NM_003681
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAGGAGTGCCGGGTGCTCTCCATACAGAGCCACGTCATCCGCGGCTACGTGGGCAACCGGGCGG
  CCACGTTCCCGCTGCAGGTTTTGGGATTTGAGATTGACGCGGTGAACTCTGTCCAGTTTTCAAACCACAC
  AGGCTATGCCCACTGGAAGGGCCAAGTGCTGAATTCAGATGAGCTCCAGGAGTTGTACGAAGGCCTGAGG
  CTGAACAACATGAATAAATATGACTACGTGCTCACAGGTTATACGAGGGACAAGTCGTTCCTGGCCATGG
  TGGTGGACATTGTGCAGGAGCTGAAGCAGCAGAACCCCAGGCTGGTGTACGTGTGTGATCCAGTCTTGGG
  TGACAAGTGGGACGGCGAAGGCTCGATGTACGTCCCGGAGGACCTCCTTCCCGTCTACAAAGAAAAAGTG
  GTGCCGCTTGCAGACATTATCACGCCCAACCAGTTTGAGGCCGAGTTACTGAGTGGCCGGAAGATCCACA
  GCCAGGAGGAAGCCTTGCGGGTGATGGACATGCTGCACTCTATGGGCCCCGACACCGTGGTCATCACCAG
  CTCCGACCTGCCATCCCCGCAGGGCAGCAACTACCTGATTGTGCTGGGGAGTCAGAGGAGGAGGAATCCC
  GCTGGCTCCGTGGTGATGGAACGCATCCGGATGGACATTCGCAAAGTGGACGCCGTCTTTGTGGGCACTG
  GGGACCTGTTTGCTGCCATGCTCCTGGCGTGGACACACAAGCACCCCAATAACCTCAAGGTGGCCTGTGA
  GAAGACCGTGTCTACCTTGCACCACGTTCTGCAGAGGACCATCCAGTGTGCAAAAGCCCAGGCCGGGGAA
  GGAGTGAGGCCCAGCCCCATGCAGCTGGAGCTGCGGATGGTGCAGAGCAAAAGGGACATCGAGGACCCAG
  AGATCGTCGTCCAGGCCACGGTGCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200975 representing NM_003681
  Red=Cloning site Green=Tags(s)
  
  MEEECRVLSIQSHVIRGYVGNRAATFPLQVLGFEIDAVNSVQFSNHTGYAHWKGQVLNSDELQELYEGLR
  LNNMNKYDYVLTGYTRDKSFLAMVVDIVQELKQQNPRLVYVCDPVLGDKWDGEGSMYVPEDLLPVYKEKV
  VPLADIITPNQFEAELLSGRKIHSQEEALRVMDMLHSMGPDTVVITSSDLPSPQGSNYLIVLGSQRRRNP
  AGSVVMERIRMDIRKVDAVFVGTGDLFAAMLLAWTHKHPNNLKVACEKTVSTLHHVLQRTIQCAKAQAGE
  GVRPSPMQLELRMVQSKRDIEDPEIVVQATVL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003681
• ORF Size: 936 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_003681.3, NP_003672.1
• RefSeq Size: 7380
• RefSeq ORF: 939
• Locus ID: 8566
• Domains: pfkB
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Vitamin B6 metabolism
• Gene Summary: The protein encoded by this gene phosphorylates vitamin B6, a step required for the conversion of vitamin B6 to pyridoxal-5-phosphate, an important cofactor in intermediary metabolism. The encoded protein is cytoplasmic and probably acts as a homodimer. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]