FOXS1 (NM_004118) Human Tagged ORF Clone

CAT#: RG201400

FOXS1 (GFP-tagged) - Human forkhead box S1 (FOXS1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: FOXS1
• Synonyms: FKHL18; FREAC10
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG201400 representing NM_004118
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCAGCAGCCTCTGCCCGGGCCTGGCGCCCCCACAACTGAGCCAACCAAGCCTCCCTACAGCTACA
  TCGCCCTTATTGCTATGGCCATCCAGAGCTCACCGGGGCAGCGGGCCACCCTCAGTGGCATCTACCGCTA
  CATCATGGGCCGATTCGCCTTCTACCGCCACAACCGGCCCGGCTGGCAGAACAGCATCCGCCACAACCTG
  TCACTCAACGAGTGCTTTGTCAAGGTGCCCCGCGATGACCGCAAGCCAGGCAAGGGCAGCTACTGGACGC
  TGGACCCTGACTGCCACGACATGTTTGAGCACGGCAGCTTCCTACGCCGCCGCCGCCGCTTCACCCGGCA
  GACAGGTGCTGAGGGCACCCGGGGCCCCGCCAAGGCACGCCGTGGACCCCTCAGGGCGACCAGCCAGGAC
  CCAGGAGTCCCCAACGCCACGACCGGCAGGCAGTGCTCATTCCCACCAGAGCTGCCAGATCCCAAGGGCC
  TAAGCTTTGGGGGTCTGGTGGGGGCCATGCCAGCCAGTATGTGCCCAGCAACCACTGATGGCAGGCCTCG
  GCCACCCATGGAGCCCAAAGAGATTTCCACGCCCAAGCCTGCATGCCCAGGGGAGCTCCCCGTGGCCACC
  TCATCTTCCTCATGCCCAGCGTTTGGCTTTCCTGCCGGCTTCTCAGAGGCTGAGAGTTTTAATAAGGCCC
  CTACGCCCGTCTTGTCCCCGGAATCAGGCATCGGGAGCAGCTACCAGTGTCGGCTGCAGGCACTGAATTT
  TTGCATGGGGGCTGACCCAGGCCTTGAGCACCTCTTGGCCTCAGCAGCCCCCTCCCCTGCACCACCCACC
  CCTCCAGGCTCACTCCGGGCCCCACTGCCCCTGCCAACTGACCACAAGGAACCCTGGGTTGCAGGTGGCT
  TCCCTGTCCAGGGAGGCTCCGGCTACCCATTGGGGCTGACCCCCTGCCTATACCGGACGCCAGGAATGTT
  CTTCTTTGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG201400 representing NM_004118
  Red=Cloning site Green=Tags(s)
  
  MQQQPLPGPGAPTTEPTKPPYSYIALIAMAIQSSPGQRATLSGIYRYIMGRFAFYRHNRPGWQNSIRHNL
  SLNECFVKVPRDDRKPGKGSYWTLDPDCHDMFEHGSFLRRRRRFTRQTGAEGTRGPAKARRGPLRATSQD
  PGVPNATTGRQCSFPPELPDPKGLSFGGLVGAMPASMCPATTDGRPRPPMEPKEISTPKPACPGELPVAT
  SSSSCPAFGFPAGFSEAESFNKAPTPVLSPESGIGSSYQCRLQALNFCMGADPGLEHLLASAAPSPAPPT
  PPGSLRAPLPLPTDHKEPWVAGGFPVQGGSGYPLGLTPCLYRTPGMFFFE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004118
• ORF Size: 990 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004118.3, NP_004109.1
• RefSeq Size: 1353 bp
• RefSeq ORF: 993 bp
• Locus ID: 2307
• Cytogenetics: 20q11.21
• Protein Families: Transcription Factors
• Gene Summary: 'The forkhead family of transcription factors belongs to the winged helix class of DNA-binding proteins. The protein encoded by this intronless gene contains a forkhead domain and is found predominantly in aorta and kidney. The function of the encoded protein is unknown. [provided by RefSeq, Jul 2008]'