ALG6 (NM_013339) Human Tagged ORF Clone

CAT#: RG201511

ALG6 (GFP-tagged) - Human asparagine-linked glycosylation 6, alpha-1,3-glucosyltransferase homolog (S. cerevisiae) (ALG6)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ALG6
• Synonyms: CDG1C
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG201511 representing NM_013339
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAAATGGTACTTGATGACAGTAGTGGTTTTAATAGGACTAACAGTACGATGGACAGTGTCTCTTA
  ATTCTTATTCAGGTGCTGGTAAACCGCCTATGTTTGGTGATTATGAAGCTCAGAGACACTGGCAAGAAAT
  AACTTTTAATTTACCGGTCAAACAATGGTATTTTAACAGCAGTGATAACAATTTACAGTATTGGGGATTG
  GATTACCCACCTCTTACAGCTTATCATAGTCTCCTATGTGCATATGTGGCAAAGTTTATAAATCCAGACT
  GGATTGCTCTCCATACATCACGTGGATATGAGAGTCAGGCACATAAGCTCTTCATGCGTACAACAGTTTT
  AATTGCTGATCTGCTGATTTACATACCTGCAGTGGTTTTGTACTGTTGTTGCTTAAAAGAAATCTCAACT
  AAGAAAAAGATTGCTAATGCATTATGCATCTTGCTGTATCCAGGCCTTATTCTTATAGACTATGGACATT
  TTCAATATAATTCTGTGAGTCTTGGCTTTGCTTTGTGGGGTGTTCTTGGAATATCTTGTGACTGCGACCT
  CCTAGGGTCACTGGCATTTTGCTTAGCTATAAATTATAAACAGATGGAACTTTACCACGCCTTGCCATTT
  TTTTGCTTTTTACTTGGCAAGTGTTTTAAAAAAGGCCTCAAAGGAAAGGGGTTTGTGTTGCTAGTTAAGC
  TAGCTTGTATTGTTGTGGCTTCCTTCGTTCTCTGCTGGCTGCCATTCTTTACAGAAAGGGAACAAACCCT
  GCAGGTTCTAAGAAGACTCTTCCCGGTTGATCGTGGATTATTTGAGGATAAAGTAGCCAATATTTGGTGC
  AGCTTCAATGTCTTTCTGAAGATTAAGGATATTTTGCCACGTCACATCCAATTAATAATGAGCTTTTGTT
  TTACGTTTTTGAGCCTGCTTCCTGCATGCATAAAATTAATACTTCAGCCCTCTTCCAAAGGATTCAAATT
  TACACTGGTTAGCTGTGCGCTATCATTCTTTTTATTTTCTTTCCAAGTACATGAAAAATCCATTCTCTTG
  GTGTCACTACCAGTCTGCTTAGTTTTAAGTGAAATTCCTTTTATGTCTACTTGGTTTTTACTTGTGTCAA
  CATTTAGTATGCTACCTCTTCTATTGAAGGATGAACTCCTAATGCCCTCTGTTGTGACAACAATGGCATT
  TTTTATAGCTTGTGTAACTTCCTTTTCAATATTTGAAAAGACTTCTGAAGAAGAACTGCAGTTGAAATCC
  TTTTCCATTTCTGTGAGGAAATATCTTCCATGTTTTACATTTCTTTCCAGAATTATACAATATTTGTTTC
  TTATCTCAGTCATCACTATGGTGCTTCTGACGTTGATGACTGTCACACTGGATCCTCCTCAGAAACTACC
  GGACTTGTTTTCTGTATTGGTGTGTTTTGTATCTTGCTTGAACTTCCTGTTCTTCTTGGTATACTTTAAC
  ATTATTATTATGTGGGATTCCAAAAGTGGAAGAAATCAGAAGAAAATCAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG201511 representing NM_013339
  Red=Cloning site Green=Tags(s)
  
  MEKWYLMTVVVLIGLTVRWTVSLNSYSGAGKPPMFGDYEAQRHWQEITFNLPVKQWYFNSSDNNLQYWGL
  DYPPLTAYHSLLCAYVAKFINPDWIALHTSRGYESQAHKLFMRTTVLIADLLIYIPAVVLYCCCLKEIST
  KKKIANALCILLYPGLILIDYGHFQYNSVSLGFALWGVLGISCDCDLLGSLAFCLAINYKQMELYHALPF
  FCFLLGKCFKKGLKGKGFVLLVKLACIVVASFVLCWLPFFTEREQTLQVLRRLFPVDRGLFEDKVANIWC
  SFNVFLKIKDILPRHIQLIMSFCFTFLSLLPACIKLILQPSSKGFKFTLVSCALSFFLFSFQVHEKSILL
  VSLPVCLVLSEIPFMSTWFLLVSTFSMLPLLLKDELLMPSVVTTMAFFIACVTSFSIFEKTSEEELQLKS
  FSISVRKYLPCFTFLSRIIQYLFLISVITMVLLTLMTVTLDPPQKLPDLFSVLVCFVSCLNFLFFLVYFN
  IIIMWDSKSGRNQKKIS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013339
• ORF Size: 1521 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013339.2, NP_037471.2
• RefSeq Size: 3371
• RefSeq ORF: 1524
• Locus ID: 29929
• Domains: Alg6_Alg8
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• Gene Summary: This gene encodes a member of the ALG6/ALG8 glucosyltransferase family. The encoded protein catalyzes the addition of the first glucose residue to the growing lipid-linked oligosaccharide precursor of N-linked glycosylation. Mutations in this gene are associated with congenital disorders of glycosylation type Ic. [provided by RefSeq, Jul 2008]