Cytokeratin 17 (KRT17) (NM_000422) Human Tagged ORF Clone

CAT#: RG201619

KRT17 (GFP-tagged) - Human keratin 17 (KRT17)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: KRT17
• Synonyms: 39.1; CK-17; K17; PC; PC2; PCHC1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG201619 representing NM_000422
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCACCTCCATCCGCCAGTTCACCTCCTCCAGCTCCATCAAGGGCTCCTCCGGCCTGGGGGGCGGCT
  CGTCCCGCACCTCCTGCCGGCTGTCTGGCGGCCTGGGTGCCGGCTCCTGCAGGCTGGGATCTGCTGGCGG
  CCTGGGCAGCACCCTCGGGGGTAGCAGCTACTCCAGCTGCTACAGCTTTGGCTCTGGTGGTGGCTATGGC
  AGCAGCTTTGGGGGTGTTGATGGGCTGCTGGCTGGAGGTGAGAAGGCCACCATGCAGAACCTCAATGACC
  GCCTGGCCTCCTACCTGGACAAGGTGCGTGCCCTGGAGGAGGCCAACACTGAGCTGGAGGTGAAGATCCG
  TGACTGGTACCAGAGGCAGGCCCCGGGGCCCGCCCGTGACTACAGCCAGTACTACAGGACAATTGAGGAG
  CTGCAGAACAAGATCCTCACAGCCACCGTGGACAATGCCAACATCCTGCTACAGATTGACAATGCCCGTC
  TGGCTGCTGATGACTTCCGCACCAAGTTTGAGACAGAGCAGGCCCTGCGCCTGAGTGTGGAGGCCGACAT
  CAATGGCCTGCGCAGGGTGCTGGATGAGCTGACCCTGGCCAGAGCCGACCTGGAGATGCAGATTGAGAAC
  CTCAAGGAGGAGCTGGCCTACCTGAAGAAGAACCACGAGGAGGAGATGAACGCCCTGCGAGGCCAGGTGG
  GTGGTGAGATCAATGTGGAGATGGACGCTGCCCCAGGCGTGGACCTGAGCCGCATCCTCAACGAGATGCG
  TGACCAGTATGAGAAGATGGCAGAGAAGAACCGCAAGGATGCCGAGGATTGGTTCTTCAGCAAGACAGAG
  GAACTGAACCGCGAGGTGGCCACCAACAGTGAGCTGGTGCAGAGTGGCAAGAGTGAGATCTCGGAGCTCC
  GGCGCACCATGCAGGCCTTGGAGATAGAGCTGCAGTCCCAGCTCAGCATGAAAGCATCCCTGGAGGGCAA
  CCTGGCGGAGACAGAGAACCGCTACTGCGTGCAGCTGTCCCAGATCCAGGGGCTGATTGGCAGCGTGGAG
  GAGCAGCTGGCCCAGCTTCGCTGCGAGATGGAGCAGCAGAACCAGGAATACAAAATCCTGCTGGATGTGA
  AGACGCGGCTGGAGCAGGAGATTGCCACCTACCGCCGCCTGCTGGAGGGAGAGGATGCCCACCTGACTCA
  GTACAAGAAAGAACCGGTGACCACCCGTCAGGTGCGTACCATTGTGGAAGAGGTCCAGGATGGCAAGGTC
  ATCTCCTCCCGCGAGCAGGTCCACCAGACCACCCGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG201619 representing NM_000422
  Red=Cloning site Green=Tags(s)
  
  MTTSIRQFTSSSSIKGSSGLGGGSSRTSCRLSGGLGAGSCRLGSAGGLGSTLGGSSYSSCYSFGSGGGYG
  SSFGGVDGLLAGGEKATMQNLNDRLASYLDKVRALEEANTELEVKIRDWYQRQAPGPARDYSQYYRTIEE
  LQNKILTATVDNANILLQIDNARLAADDFRTKFETEQALRLSVEADINGLRRVLDELTLARADLEMQIEN
  LKEELAYLKKNHEEEMNALRGQVGGEINVEMDAAPGVDLSRILNEMRDQYEKMAEKNRKDAEDWFFSKTE
  ELNREVATNSELVQSGKSEISELRRTMQALEIELQSQLSMKASLEGNLAETENRYCVQLSQIQGLIGSVE
  EQLAQLRCEMEQQNQEYKILLDVKTRLEQEIATYRRLLEGEDAHLTQYKKEPVTTRQVRTIVEEVQDGKV
  ISSREQVHQTTR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000422
• ORF Size: 1296 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000422.1, NP_000413.1
• RefSeq Size: 1512 bp
• RefSeq ORF: 1299 bp
• Locus ID: 3872
• Cytogenetics: 17q21.2
• Gene Summary: 'This gene encodes the type I intermediate filament chain keratin 17, expressed in nail bed, hair follicle, sebaceous glands, and other epidermal appendages. Mutations in this gene lead to Jackson-Lawler type pachyonychia congenita and steatocystoma multiplex. [provided by RefSeq, Aug 2008]'