THOC3 (NM_032361) Human Tagged ORF Clone

CAT#: RG201698

THOC3 (GFP-tagged) - Human THO complex 3 (THOC3)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: THOC3
• Synonyms: hTREX45; THO3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG201698 representing NM_032361
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGTCCCCGCTGCAGCCATGGGGCCCTCGGCGTTGGGCCAGAGCGGCCCCGGCTCGATGGCCCCGT
  GGTGCTCAGTGAGCAGCGGCCCGTCGCGCTACGTGCTTGGGATGCAGGAGCTGTTCCGGGGCCACAGCAA
  GACGCGCGAGTTCCTGGCGCACAGCGCCAAGGTGCACTCGGTGGCCTGGAGTTGCGACGGGCGTCGCCTA
  GCCTCGGGGTCCTTCGACAAGACGGCCAGCGTCTTCTTGCTGGAGAAGGACCGGTTGGTCAAAGAAAACA
  ATTATCGGGGACATGGGGATAGTGTGGACCAGCTTTGTTGGCATCCAAGTAATCCTGACCTATTTGTTAC
  GGCGTCTGGAGATAAAACCATTCGCATCTGGGATGTGAGGACTACAAAATGCATTGCCACTGTGAACACT
  AAAGGGGAGAACATTAATATCTGCTGGAGTCCTGATGGGCAGACCATTGCTGTAGGCAACAAGGATGATG
  TGGTGACCTTTATTGATGCCAAGACACACCGTTCCAAAGCAGAAGAGCAGTTCAAGTTCGAGGTCAACGA
  AATCTCCTGGAACAATGACAATAATATGTTCTTCCTGACAAATGGCAATGGTTGTATCAACATCCTCAGC
  TACCCAGAACTGAAGCCTGTGCAGTCCATCAACGCCCATCCTTCCAACTGCATCTGTATCAAGTTTGACC
  CCATGGGGAAGTACTTTGCCACAGGAAGTGCAGATGCTTTGGTCAGCCTCTGGGATGTGGATGAGTTAGT
  GTGTGTTCGGTGCTTTTCCAGGCTGGATTGGCCTGTAAGAACCCTCAGTTTCAGCCATGATGGGAAAATG
  CTGGCGTCAGCATCGGAAGATCATTTTATTGACATTGCTGAAGTGGAGACAGGGGACAAACTATGGGAGG
  TACAGTGTGAGTCTCCGACCTTCACAGTGGCGTGGCACCCCAAAAGGCCTCTGCTGGCATTTGCCTGTGA
  TGACAAAGACGGCAAATATGACAGCAGCCGGGAAGCCGGAACTGTGAAGCTGTTTGGGCTTCCTAATGAT
  TCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG201698 representing NM_032361
  Red=Cloning site Green=Tags(s)
  
  MAVPAAAMGPSALGQSGPGSMAPWCSVSSGPSRYVLGMQELFRGHSKTREFLAHSAKVHSVAWSCDGRRL
  ASGSFDKTASVFLLEKDRLVKENNYRGHGDSVDQLCWHPSNPDLFVTASGDKTIRIWDVRTTKCIATVNT
  KGENINICWSPDGQTIAVGNKDDVVTFIDAKTHRSKAEEQFKFEVNEISWNNDNNMFFLTNGNGCINILS
  YPELKPVQSINAHPSNCICIKFDPMGKYFATGSADALVSLWDVDELVCVRCFSRLDWPVRTLSFSHDGKM
  LASASEDHFIDIAEVETGDKLWEVQCESPTFTVAWHPKRPLLAFACDDKDGKYDSSREAGTVKLFGLPND
  S
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032361
• ORF Size: 1053 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_032361.1, NP_115737.1
• RefSeq Size: 1577
• RefSeq ORF: 1056
• Locus ID: 84321
• Domains: WD40
• Protein Pathways: Spliceosome
• Gene Summary: This gene encodes a component of the nuclear THO transcription elongation complex, which is part of the larger transcription export (TREX) complex that couples messenger RNA processing and export. In humans, the transcription export complex is recruited to the 5'-end of messenger RNAs in a splicing- and cap-dependent manner. Studies of a related complex in mouse suggest that the metazoan transcription export complex is involved in cell differentiation and development. A pseudogene of this gene has been defined on chromosome 5. [provided by RefSeq, May 2013]