Acylglycerol Kinase (AGK) (NM_018238) Human Tagged ORF Clone

CAT#: RG201891

AGK (GFP-tagged) - Human acylglycerol kinase (AGK), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: AGK
• Synonyms: CATC5; CTRCT38; MTDPS10; MULK
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG201891 representing NM_018238
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGTGTTCTTTAAAACGCTTCGAAATCACTGGAAGAAAACTACAGCTGGGCTCTGCCTGCTGACCT
  GGGGAGGCCATTGGCTCTATGGAAAACACTGTGATAACCTCCTAAGGAGAGCAGCCTGTCAAGAAGCTCA
  GGTGTTTGGCAATCAACTCATTCCTCCCAATGCACAAGTGAAGAAGGCCACTGTTTTTCTCAATCCTGCA
  GCTTGCAAAGGAAAAGCTAGGACTCTATTTGAAAAAAATGCTGCCCCGATTTTACATTTATCTGGCATGG
  ATGTGACTATTGTTAAGACAGATTATGAGGGACAAGCCAAGAAACTCCTGGAACTGATGGAAAACACGGA
  TGTGATCATTGTTGCAGGAGGAGATGGGACACTGCAGGAGGTTGTTACTGGTGTTCTTCGACGAACAGAT
  GAGGCTACCTTCAGTAAGATTCCCATTGGATTTATCCCACTGGGAGAGACCAGTAGTTTGAGTCATACCC
  TCTTTGCCGAAAGTGGAAACAAAGTCCAACATATTACTGATGCCACACTTGCCATTGTGAAAGGAGAGAC
  AGTTCCACTTGATGTCTTGCAGATCAAGGGTGAAAAGGAACAGCCTGTATTTGCAATGACCGGCCTTCGA
  TGGGGATCTTTCAGAGATGCTGGCGTCAAAGTTAGCAAGTACTGGTATCTTGGGCCTCTAAAAATCAAAG
  CAGCCCACTTTTTCAGCACTCTTAAGGAGTGGCCTCAGACTCATCAAGCCTCTATCTCATACACGGGACC
  TACAGAGAGACCTCCCAATGAACCAGAGGAGACCCCTGTACAAAGGCCTTCTTTGTACAGGAGAATATTA
  CGAAGGCTTGCGTCCTACTGGGCACAACCACAGGATGCCCTTTCCCAAGAGGTGAGCCCGGAGGTCTGGA
  AAGATGTGCAGCTGTCCACCATTGAACTGTCCATCACAACACGGAATAATCAGCTTGACCCGACAAGCAA
  AGAAGATTTTCTGAATATCTGCATTGAACCTGACACCATCAGCAAAGGAGACTTTATAACTATAGGAAGT
  CGAAAGGTGAGAAACCCCAAGCTGCACGTGGAGGGCACGGAGTGTCTCCAAGCCAGCCAGTGCACTTTGC
  TTATCCCGGAGGGAGCAGGGGGCTCTTTTAGCATTGACAGTGAGGAGTATGAAGCGATGCCTGTGGAGGT
  GAAACTGCTCCCCAGGAAGCTGCAGTTCTTCTGTGATCCTAGGAAGAGAGAACAGATGCTCACAAGCCCC
  ACCCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG201891 representing NM_018238
  Red=Cloning site Green=Tags(s)
  
  MTVFFKTLRNHWKKTTAGLCLLTWGGHWLYGKHCDNLLRRAACQEAQVFGNQLIPPNAQVKKATVFLNPA
  ACKGKARTLFEKNAAPILHLSGMDVTIVKTDYEGQAKKLLELMENTDVIIVAGGDGTLQEVVTGVLRRTD
  EATFSKIPIGFIPLGETSSLSHTLFAESGNKVQHITDATLAIVKGETVPLDVLQIKGEKEQPVFAMTGLR
  WGSFRDAGVKVSKYWYLGPLKIKAAHFFSTLKEWPQTHQASISYTGPTERPPNEPEETPVQRPSLYRRIL
  RRLASYWAQPQDALSQEVSPEVWKDVQLSTIELSITTRNNQLDPTSKEDFLNICIEPDTISKGDFITIGS
  RKVRNPKLHVEGTECLQASQCTLLIPEGAGGSFSIDSEEYEAMPVEVKLLPRKLQFFCDPRKREQMLTSP
  TQ
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018238
• ORF Size: 1266 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_018238.2, NP_060708.1
• RefSeq Size: 2794
• RefSeq ORF: 1269
• Locus ID: 55750
• Domains: DAGKc
• Protein Pathways: Glycerolipid metabolism, Metabolic pathways
• Gene Summary: The protein encoded by this gene is a mitochondrial membrane protein involved in lipid and glycerolipid metabolism. The encoded protein is a lipid kinase that catalyzes the formation of phosphatidic and lysophosphatidic acids. Defects in this gene have been associated with mitochondrial DNA depletion syndrome 10. [provided by RefSeq, Feb 2012]