SH3KBP1 (NM_031892) Human Tagged ORF Clone

CAT#: RG202186

SH3KBP1 (GFP-tagged) - Human SH3-domain kinase binding protein 1 (SH3KBP1), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SH3KBP1
• Synonyms: AGMX2; CD2BP3; CIN85; GIG10; HSB-1; HSB1; IMD61; MIG18
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG202186 representing NM_031892
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGAGGCCATAGTGGAGTTTGACTACCAGGCCCAGCACGATGATGAGCTGACGATCAGCGTGGGTG
  AAATCATCACCAACATCAGGAAGGAGGATGGAGGCTGGTGGGAGGGACAGATCAACGGCAGGAGAGGTTT
  GTTCCCTGACAACTTTGTAAGAGAAATAAAGAAAGAGATGAAGAAAGACCCTCTCACCAACAAAGCTCCA
  GAAAAGCCCCTGCACGAAGTGCCCAGTGGAAACTCTTTGCTGTCTTCTGAAACGATTTTAAGAACCAATA
  AGAGAGGCGAGCGACGGAGGCGCCGGTGCCAGGTGGCATTCAGCTACCTGCCCCAGAATGACGATGAACT
  TGAGCTGAAAGTTGGCGACATCATAGAGGTGGTAGGAGAGGTAGAGGAAGGATGGTGGGAAGGTGTTCTC
  AACGGGAAGACTGGAATGTTTCCTTCCAACTTCATCAAGGAGCTGTCAGGGGAGTCGGATGAGCTTGGCA
  TTTCCCAGGATGAGCAGCTATCCAAGTCAAGTTTAAGGGAAACCACAGGCTCCGAGAGTGATGGGGGTGA
  CTCAAGCAGCACCAAGTCTGAAGGTGCCAACGGGACAGTGGCAACTGCAGCAATCCAGCCCAAGAAAGTT
  AAGGGAGTGGGCTTTGGAGACATTTTCAAAGACAAGCCAATCAAACTAAGACCAAGGTCAATTGAAGTAG
  AAAATGACTTTCTGCCGGTAGAAAAGACTATTGGGAAGAAGTTACCTGCAACTACAGCAACTCCAGACTC
  ATCAAAAACAGAAATGGACAGCAGGACAAAGAGCAAGGATTACTGCAAAGTAATATTTCCATATGAGGCA
  CAGAATGATGATGAATTGACAATCAAAGAAGGAGATATAGTCACTCTCATCAATAAGGACTGCATCGACG
  TAGGCTGGTGGGAAGGAGAGCTGAACGGCAGACGAGGCGTGTTCCCCGATAACTTCGTGAAGTTACTTCC
  ACCGGACTTTGAAAAGGAAGGGAATAGACCCAAGAAGCCACCGCCTCCATCCGCTCCTGTCATCAAACAA
  GGGGCAGGCACCACTGAGAGAAAACATGAAATTAAAAAGATACCTCCTGAAAGACCAGAAATGCTTCCAA
  ACAGAACAGAAGAAAAAGAAAGACCAGAGAGAGAGCCAAAACTGGATTTACAGAAGCCCTCCGTTCCTGC
  CATACCGCCAAAAAAGCCTCGGCCACCTAAGACCAATTCTCTCAGCAGACCTGGCGCACTGCCCCCGAGA
  AGGCCGGAGAGACCGGTGGGTCCGCTGACACACACCAGGGGTGACAGTCCAAAGATTGACTTGGCCGGCA
  GTTCGCTATCTGGCATCCTGGACAAAGATCTCTCGGACCGCAGCAATGACATTGACTTAGAAGGTTTTGA
  CTCCGTGGTATCATCTACTGAGAAACTCAGTCATCCGACCACAAGCAGACCAAAAGCTACAGGGAGGCGG
  CCTCCGTCCCAGTCCCTCACATCTTCATCCCTTTCAAGCCCTGATATCTTCGACTCCCCAAGTCCCGAAG
  AGGATAAGGAGGAACACATTTCACTTGCGCACAGAGGAGTGGACGCGTCAAAGAAAACTTCCAAGACTGT
  TACCATATCCCAAGTGTCTGACAACAAAGCATCCCTGCCGCCCAAGCCGGGGACCATGGCAGCAGGTGGC
  GGTGGGCCAGCCCCTCTGTCCTCAGCGGTGCCCTCCCCCCTGTCATCCTCTTTGGGAACAGCTGGACACA
  GAGCCAACTCCCCGTCTCTGTTCGGCACGGAAGGAAAACCAAAGATGGAGCCTGCGGCCAGCAGCCAGGC
  GGCCGTGGAGGAGCTAAGGACACAGGTCCGCGAGCTGAGGAGCATCATCGAGACCATGAAGGACCAGCAG
  AAACGAGAGATTAAACAGTTATTGTCTGAGTTGGATGAAGAGAAGAAAATCCGGCTTCGGTTGCAGATGG
  AAGTGAACGACATAAAGAAAGCTCTACAATCAAAA
  
  ACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG202186 representing NM_031892
  Red=Cloning site Green=Tags(s)
  
  MVEAIVEFDYQAQHDDELTISVGEIITNIRKEDGGWWEGQINGRRGLFPDNFVREIKKEMKKDPLTNKAP
  EKPLHEVPSGNSLLSSETILRTNKRGERRRRRCQVAFSYLPQNDDELELKVGDIIEVVGEVEEGWWEGVL
  NGKTGMFPSNFIKELSGESDELGISQDEQLSKSSLRETTGSESDGGDSSSTKSEGANGTVATAAIQPKKV
  KGVGFGDIFKDKPIKLRPRSIEVENDFLPVEKTIGKKLPATTATPDSSKTEMDSRTKSKDYCKVIFPYEA
  QNDDELTIKEGDIVTLINKDCIDVGWWEGELNGRRGVFPDNFVKLLPPDFEKEGNRPKKPPPPSAPVIKQ
  GAGTTERKHEIKKIPPERPEMLPNRTEEKERPEREPKLDLQKPSVPAIPPKKPRPPKTNSLSRPGALPPR
  RPERPVGPLTHTRGDSPKIDLAGSSLSGILDKDLSDRSNDIDLEGFDSVVSSTEKLSHPTTSRPKATGRR
  PPSQSLTSSSLSSPDIFDSPSPEEDKEEHISLAHRGVDASKKTSKTVTISQVSDNKASLPPKPGTMAAGG
  GGPAPLSSAVPSPLSSSLGTAGHRANSPSLFGTEGKPKMEPAASSQAAVEELRTQVRELRSIIETMKDQQ
  KREIKQLLSELDEEKKIRLRLQMEVNDIKKALQSK
  
  TRPLE - GFP Tag - V
• Restriction Sites: SgfI-NotI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031892
• ORF Size: 1995 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031892.1, NP_114098.1
• RefSeq Size: 3348
• RefSeq ORF: 1998
• Locus ID: 30011
• Domains: SH3
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis
• Gene Summary: This gene encodes an adapter protein that contains one or more N-terminal Src homology domains, a proline rich region and a C-terminal coiled-coil domain. The encoded protein facilitates protein-protein interactions and has been implicated in numerous cellular processes including apoptosis, cytoskeletal rearrangement, cell adhesion and in the regulation of clathrin-dependent endocytosis. Alternate splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2017]