NEIL1 (NM_024608) Human Tagged ORF Clone

CAT#: RG202329

NEIL1 (GFP-tagged) - Human nei endonuclease VIII-like 1 (E. coli) (NEIL1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NEIL1
• Synonyms: FPG1; hFPG1; NEI1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG202329 representing NM_024608
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGAGGGCCCCGAGCTGCACCTGGCCAGCCAGTTTGTGAATGAGGCCTGCAGGGCGCTGGTGTTCG
  GCGGCTGCGTGGAGAAGTCCTCTGTCAGCCGCAACCCTGAGGTGCCCTTTGAGAGCAGTGCCTACCGCAT
  CTCAGCTTCAGCCCGCGGCAAGGAGCTGCGCCTGATACTGAGCCCTCTGCCTGGGGCCCAGCCCCAACAG
  GAGCCACTGGCCCTGGTCTTCCGCTTCGGCATGTCCGGCTCTTTTCAGCTGGTGCCCCGCGAGGAGCTGC
  CACGCCATGCCCACCTGCGCTTTTACACGGCCCCGCCTGGCCCCCGGCTCGCCCTATGTTTCGTGGACAT
  CCGCCGGTTCGGCCGCTGGGACCTTGGGGGAAAGTGGCAGCCGGGCCGCGGGCCCTGTGTCTTGCAGGAG
  TACCAGCAGTTCAGGGAGAATGTGCTACGAAACCTAGCGGATAAGGCCTTTGACCGGCCCATCTGCGAGG
  CCCTCCTGGACCAGAGGTTCTTCAATGGCATTGGCAACTATCTGCGGGCAGAGATCCTGTACCGGCTGAA
  GATCCCCCCCTTTGAGAAGGCCCGCTCGGTCCTGGAGGCCCTGCAGCAGCACAGGCCGAGCCCGGAGCTG
  ACCCTGAGCCAGAAGATAAGGACCAAGCTGCAGAATCCAGACCTGCTGGAGCTATGTCACTCAGTGCCCA
  AGGAAGTGGTCCAGTTGGGGGGCAGGGGCTACGGGTCAGAGAGCGGGGAGGAGGACTTTGCTGCCTTTCG
  AGCCTGGCTGCGCTGCTATGGCATGCCAGGCATGAGCTCCCTGCAGGACCGGCATGGCCGTACCATCTGG
  TTCCAGGGGGATCCTGGACCGTTGGCACCCAAAGGGCGCAAGTCCCGCAAAAAGAAATCCAAGGCCACAC
  AGCTGAGTCCTGAGGACAGAGTGGAGGACGCTTTGCCTCCAAGCAAGGCCCCTTCCAGGACACGAAGGGC
  AAAGAGAGACCTTCCTAAGAGGACTGCAACCCAGCGGCCTGAGGGGACCAGCCTCCAGCAGGACCCAGAA
  GCTCCCACAGTGCCCAAGAAGGGGAGGAGGAAGGGGCGACAGGCAGCCTCTGGCCACTGCAGACCCCGGA
  AGGTCAAGGCTGACATCCCATCCTTGGAACCAGAGGGGACCTCAGCCTCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG202329 representing NM_024608
  Red=Cloning site Green=Tags(s)
  
  MPEGPELHLASQFVNEACRALVFGGCVEKSSVSRNPEVPFESSAYRISASARGKELRLILSPLPGAQPQQ
  EPLALVFRFGMSGSFQLVPREELPRHAHLRFYTAPPGPRLALCFVDIRRFGRWDLGGKWQPGRGPCVLQE
  YQQFRENVLRNLADKAFDRPICEALLDQRFFNGIGNYLRAEILYRLKIPPFEKARSVLEALQQHRPSPEL
  TLSQKIRTKLQNPDLLELCHSVPKEVVQLGGRGYGSESGEEDFAAFRAWLRCYGMPGMSSLQDRHGRTIW
  FQGDPGPLAPKGRKSRKKKSKATQLSPEDRVEDALPPSKAPSRTRRAKRDLPKRTATQRPEGTSLQQDPE
  APTVPKKGRRKGRQAASGHCRPRKVKADIPSLEPEGTSAS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024608
• ORF Size: 1170 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_024608.1, NP_078884.1
• RefSeq Size: 1828
• RefSeq ORF: 1173
• Locus ID: 79661
• Domains: Fapy_DNA_glyco
• Protein Families: Druggable Genome
• Protein Pathways: Base excision repair
• Gene Summary: This gene is a member of the Nei endonuclease VIII-like gene family which encodes DNA glycosylases. The encoded enzyme participates in the DNA repair pathway by initiating base excision repair by removing damaged bases, primarily oxidized pyrimidines. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]