AP1M1 (NM_032493) Human Tagged ORF Clone

CAT#: RG202366

AP1M1 (GFP-tagged) - Human adaptor-related protein complex 1, mu 1 subunit (AP1M1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: AP1M1
• Synonyms: AP47; CLAPM2; CLTNM; MU-1A
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG202366 representing NM_032493
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGCCAGCGCCGTCTACGTGCTGGACCTGAAGGGCAAGGTGCTCATCTGCCGGAACTACCGTGGCG
  ACGTGGACATGTCAGAGGTGGAGCACTTCATGCCCATCCTGATGGAGAAGGAGGAGGAGGGGATGCTGTC
  GCCCATCCTGGCCCACGGGGGGGTCCGTTTCATGTGGATCAAACACAACAACCTGTATCTGGTTGCCACA
  TCCAAGAAGAACGCGTGCGTGTCGCTGGTCTTTTCTTTCCTCTATAAGGTGGTGCAGGTGTTTTCCGAGT
  ACTTCAAGGAGCTGGAGGAGGAGAGCATCCGGGACAACTTTGTTATCATCTACGAGCTGCTGGACGAGCT
  CATGGACTTCGGCTACCCCCAGACCACCGACAGCAAGATCCTGCAGGAGTACATCACTCAGGAAGGCCAC
  AAGCTGGAAACAGGGGCCCCGCGGCCACCAGCCACCGTCACCAACGCGGTGTCCTGGCGGTCCGAAGGCA
  TCAAGTATCGGAAGAATGAGGTGTTCTTGGACGTCATCGAGTCTGTCAACCTCTTGGTCAGCGCCAACGG
  CAATGTCCTGCGCAGCGAGATCGTGGGCTCCATCAAGATGCGAGTCTTCCTCTCGGGCATGCCCGAGCTG
  CGCCTGGGCCTCAACGACAAGGTCCTCTTTGACAACACGGGCCGCGGCAAAAGCAAATCCGTGGAGCTGG
  AGGATGTGAAGTTCCACCAGTGTGTGCGGCTATCACGCTTCGAGAATGACCGCACCATCTCCTTCATCCC
  ACCCGACGGCGAGTTCGAGCTCATGTCCTACCGTCTCAACACCCACGTCAAGCCTTTGATATGGATCGAG
  TCGGTGATCGAGAAGCACTCCCACAGCCGCATCGAGTACATGATCAAGGCCAAAAGCCAGTTCAAGCGGC
  GGTCAACAGCCAACAACGTGGAGATCCACATTCCCGTGCCCAATGATGCCGACTCACCCAAGTTCAAGAC
  GACGGTGGGGAGCGTTAAGTGGGTCCCCGAGAACAGCGAGATTGTGTGGTCCATCAAGTCCTTCCCGGGC
  GGCAAGGAGTACCTGATGCGGGCCCACTTCGGCCTGCCTAGTGTGGAGGCCGAAGACAAGGAGGGCAAGC
  CCCCGATCAGTGTCAAGTTCGAGATCCCTTACTTCACTACCTCCGGCATCCAGGTGCGCTACCTGAAGAT
  CATTGAGAAGAGTGGGTACCAGGCCCTGCCCTGGGTGCGTTATATCACGCAGAATGGAGATTACCAGCTC
  CGGACCCAG
  
  ACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG202366 representing NM_032493
  Red=Cloning site Green=Tags(s)
  
  MSASAVYVLDLKGKVLICRNYRGDVDMSEVEHFMPILMEKEEEGMLSPILAHGGVRFMWIKHNNLYLVAT
  SKKNACVSLVFSFLYKVVQVFSEYFKELEEESIRDNFVIIYELLDELMDFGYPQTTDSKILQEYITQEGH
  KLETGAPRPPATVTNAVSWRSEGIKYRKNEVFLDVIESVNLLVSANGNVLRSEIVGSIKMRVFLSGMPEL
  RLGLNDKVLFDNTGRGKSKSVELEDVKFHQCVRLSRFENDRTISFIPPDGEFELMSYRLNTHVKPLIWIE
  SVIEKHSHSRIEYMIKAKSQFKRRSTANNVEIHIPVPNDADSPKFKTTVGSVKWVPENSEIVWSIKSFPG
  GKEYLMRAHFGLPSVEAEDKEGKPPISVKFEIPYFTTSGIQVRYLKIIEKSGYQALPWVRYITQNGDYQL
  RTQ
  
  TRPLE - GFP Tag - V
• Restriction Sites: SgfI-NotI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032493
• ORF Size: 1269 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_032493.2, NP_115882.1
• RefSeq Size: 2297
• RefSeq ORF: 1272
• Locus ID: 8907
• Domains: Adap_comp_sub
• Protein Pathways: Lysosome
• Gene Summary: The protein encoded by this gene is the medium chain of the trans-Golgi network clathrin-associated protein complex AP-1. The other components of this complex are beta-prime-adaptin, gamma-adaptin, and the small chain AP1S1. This complex is located at the Golgi vesicle and links clathrin to receptors in coated vesicles. These vesicles are involved in endocytosis and Golgi processing. Alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]