GPSN2 (TECR) (NM_138501) Human Tagged ORF Clone

CAT#: RG202503

TECR (GFP-tagged) - Human trans-2,3-enoyl-CoA reductase (TECR), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TECR
• Synonyms: GPSN2; MRT14; SC2; TER
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG202503 representing NM_138501
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCATTACGAGGTGGAGATTCTGGACGCAAAGACAAGGGAGAAGCTGTGTTTCTTGGACAAGGTGG
  AGCCCCACGCCACCATTGCGGAGATCAAGAACCTCTTCACTAAGACCCATCCGCAGTGGTACCCCGCCCG
  CCAGTCCCTCCGCCTGGACCCCAAGGGCAAGTCCCTGAAGGATGAGGATGTTCTGCAGAAGCTGCCCGTG
  GGCACCACGGCCACACTGTACTTCCGGGACCTGGGGGCCCAGATCAGCTGGGTGACGGTCTTCCTAACAG
  AGTACGCGGGGCCCCTTTTCATCTACCTGCTCTTCTACTTCCGAGTGCCCTTCATCTATGGCCACAAATA
  TGACTTTACGTCCAGTCGGCATACAGTGGTGCACCTCGCCTGCATCTGTCACTCATTCCACTACATCAAG
  CGCCTGCTGGAGACGCTCTTCGTGCACCGCTTCTCCCATGGCACTATGCCTTTGCGCAACATCTTCAAGA
  ACTGCACCTACTACTGGGGCTTCGCCGCGTGGATGGCCTATTACATCAATCACCCTCTCTACACTCCCCC
  TACCTACGGAGCTCAGCAGGTGAAACTGGCGCTCGCCATCTTTGTGATCTGCCAGCTCGGCAACTTCTCC
  ATCCACATGGCCCTGCGGGACCTGCGGCCCGCTGGGTCCAAGACGCGGAAGATCCCATACCCCACCAAGA
  ACCCCTTCACGTGGCTCTTCCTGCTGGTGTCCTGCCCCAACTACACCTACGAGGTGGGGTCCTGGATCGG
  TTTCGCCATCATGACGCAGTGTCTCCCAGTGGCCCTGTTCTCCCTGGTGGGCTTCACCCAGATGACCATC
  TGGGCCAAGGGCAAGCACCGCAGCTACCTGAAGGAGTTCCGGGACTACCCGCCCCTGCGCATGCCCATCA
  TCCCCTTCCTGCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG202503 representing NM_138501
  Red=Cloning site Green=Tags(s)
  
  MKHYEVEILDAKTREKLCFLDKVEPHATIAEIKNLFTKTHPQWYPARQSLRLDPKGKSLKDEDVLQKLPV
  GTTATLYFRDLGAQISWVTVFLTEYAGPLFIYLLFYFRVPFIYGHKYDFTSSRHTVVHLACICHSFHYIK
  RLLETLFVHRFSHGTMPLRNIFKNCTYYWGFAAWMAYYINHPLYTPPTYGAQQVKLALAIFVICQLGNFS
  IHMALRDLRPAGSKTRKIPYPTKNPFTWLFLLVSCPNYTYEVGSWIGFAIMTQCLPVALFSLVGFTQMTI
  WAKGKHRSYLKEFRDYPPLRMPIIPFLL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138501
• ORF Size: 924 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_138501.4, NP_612510.1
• RefSeq Size: 1197
• RefSeq ORF: 927
• Locus ID: 9524
• Domains: Steroid_dh
• Protein Families: Transmembrane
• Protein Pathways: Biosynthesis of unsaturated fatty acids
• Gene Summary: This gene encodes a multi-pass membrane protein that resides in the endoplasmic reticulum, and belongs to the steroid 5-alpha reductase family. The elongation of microsomal long and very long chain fatty acid consists of 4 sequential reactions. This protein catalyzes the final step, reducing trans-2,3-enoyl-CoA to saturated acyl-CoA. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2011]