RFK (NM_018339) Human Tagged ORF Clone

CAT#: RG202812

RFK (GFP-tagged) - Human riboflavin kinase (RFK)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RFK
• Synonyms: RIFK
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG202812 representing NM_018339
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCGAGCGGACTGCATTATGAGGCACCTGCCTTACTTCTGCCGGGGTCAAGTGGTGCGGGGCTTCG
  GCCGCGGCTCCAAGCAGCTGGGCATCCCCACAGCTAATTTTCCTGAGCAAGTGGTAGATAATCTTCCAGC
  TGATATATCCACTGGTATTTACTATGGTTGGGCCAGTGTTGGAAGTGGAGATGTCCATAAGATGGTGGTG
  AGCATAGGATGGAACCCATATTACAAGAATACGAAGAAGTCTATGGAAACACATATCATGCATACCTTCA
  AAGAGGACTTCTATGGGGAAATCCTCAATGTCGCCATTGTTGGCTACCTGAGACCAGAAAAGAACTTTGA
  TTCTTTAGAGTCACTTATTTCAGCAATTCAAGGTGATATTGAAGAAGCTAAGAAACGACTAGAGTTACCA
  GAACATTTGAAAATCAAAGAAGACAATTTCTTCCAGGTTTCTAAAAGCAAAATAATGAATGGCCAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG202812 representing NM_018339
  Red=Cloning site Green=Tags(s)
  
  MPRADCIMRHLPYFCRGQVVRGFGRGSKQLGIPTANFPEQVVDNLPADISTGIYYGWASVGSGDVHKMVV
  SIGWNPYYKNTKKSMETHIMHTFKEDFYGEILNVAIVGYLRPEKNFDSLESLISAIQGDIEEAKKRLELP
  EHLKIKEDNFFQVSKSKIMNGH
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018339
• ORF Size: 486 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_018339.3, NP_060809.2
• RefSeq Size: 2712
• RefSeq ORF: 468
• Locus ID: 55312
• Domains: FAD_Synth
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Riboflavin metabolism
• Gene Summary: Riboflavin kinase (RFK; EC 2.7.1.26) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis (Karthikeyan et al., 2003 [PubMed 12623014]). [supplied by OMIM, Nov 2009]