ABI3 (NM_016428) Human Tagged ORF Clone

CAT#: RG202853

ABI3 (GFP-tagged) - Human ABI family, member 3 (ABI3), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ABI3
• Synonyms: NESH; SSH3BP3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG202853 representing NM_016428
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCTACAGCAGCTGCAGGAGTTTGAGATCCCCACTGGCCGGGAGGCTCTGAGGGGCAACCACA
  GTGCCCTGCTGCGGGTCGCTGACTACTGCGAGGACAACTATGTGCAGGCCACAGACAAGCGGAAGGCGCT
  GGAGGAGACCATGGCCTTCACTACCCAGGCACTGGCCAGCGTGGCCTACCAGGTGGGCAACCTGGCCGGG
  CACACTCTGCGCATGTTGGACCTGCAGGGGGCCGCCCTGCGGCAGGTGGAAGCCCGTGTAAGCACGCTGG
  GCCAGATGGTGAACATGCATATGGAGAAGGTGGCCCGAAGGGAGATCGGCACCTTAGCCACTGTCCAGCG
  GCTGCCCCCCGGCCAGAAGGTCATCGCCCCAGAGAACCTACCCCCTCTCACGCCCTACTGCAGGAGACCC
  CTCAACTTTGGCTGCCTGGACGACATTGGCCATGGGATCAAGGACCTCAGCACGCAGCTGTCAAGAACAG
  GCACCCTGTCTCGAAAGAGCATCAAGGCCCCTGCCACACCCGCCTCCGCCACCTTGGGGAGACCACCCCG
  GATTCCCGAGCCAGTGCACCTGCCGGTGGTGCCCGACGGCAGACTCTCCGCCGCCTCCTCTGCGTCTTCC
  CTGGCCTCGGCCGGCAGCGCCGAAGGTGTCGGTGGGGCCCCCACGCCCAAGGGGCAGGCAGCACCTCCAG
  CCCCACCTCTCCCCAGCTCCTTGGACCCACCTCCTCCACCAGCAGCCGTCGAGGTGTTCCAGCGGCCTCC
  CACGCTGGAGGAGTTGTCCCCACCCCCACCGGACGAAGAGCTGCCCCTGCCACTGGACCTGCCTCCTCCT
  CCACCCCTGGATGGAGATGAATTGGGGCTGCCTCCACCCCCACCAGGATTTGGGCCTGATGAGCCCAGCT
  GGGTGCCTGCCTCATACTTGGAGAAAGTGGTGACACTGTACCCATACACCAGCCAGAAGGACAATGAGCT
  CTCCTTCTCTGAGGGCACTGTCATCTGTGTCACTCGCCGCTACTCCGATGGCTGGTGCGAGGGCGTCAGC
  TCAGAGGGGACTGGATTCTTCCCTGGGAACTATGTGGAGCCCAGCTGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG202853 representing NM_016428
  Red=Cloning site Green=Tags(s)
  
  MAELQQLQEFEIPTGREALRGNHSALLRVADYCEDNYVQATDKRKALEETMAFTTQALASVAYQVGNLAG
  HTLRMLDLQGAALRQVEARVSTLGQMVNMHMEKVARREIGTLATVQRLPPGQKVIAPENLPPLTPYCRRP
  LNFGCLDDIGHGIKDLSTQLSRTGTLSRKSIKAPATPASATLGRPPRIPEPVHLPVVPDGRLSAASSASS
  LASAGSAEGVGGAPTPKGQAAPPAPPLPSSLDPPPPPAAVEVFQRPPTLEELSPPPPDEELPLPLDLPPP
  PPLDGDELGLPPPPPGFGPDEPSWVPASYLEKVVTLYPYTSQKDNELSFSEGTVICVTRRYSDGWCEGVS
  SEGTGFFPGNYVEPSC
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016428
• ORF Size: 1098 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_016428.2, NP_057512.1
• RefSeq Size: 2129
• RefSeq ORF: 1101
• Locus ID: 51225
• Domains: SH3
• Gene Summary: This gene encodes a member of an adaptor protein family. Members of this family encode proteins containing a homeobox homology domain, proline rich region and Src-homology 3 (SH3) domain, and are components of the Abi/WAVE complex which regulates actin polymerization. The encoded protein inhibits ectopic metastasis of tumor cells as well as cell migration. This may be accomplished through interaction with p21-activated kinase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]