alpha Internexin (INA) (NM_032727) Human Tagged ORF Clone

CAT#: RG202877

INA (GFP-tagged) - Human internexin neuronal intermediate filament protein, alpha (INA)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: INA
• Synonyms: NEF5; NF-66; TXBP-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG202877 representing NM_032727
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTTCGGCTCGGAGCACTACCTGTGCTCCTCCTCCTCCTACCGCAAGGTGTTCGGGGATGGCTCTC
  GCCTGTCCGCCCGCCTCTCTGGGGCCGGCGGCGCGGGCGGCTTCCGCTCGCAGTCGCTGTCCCGCAGCAA
  TGTGGCCTCCTCGGCCGCCTGCTCCTCGGCCTCGTCGCTCGGCCTCGGCCTGGCCTATCGCCGGCCGCCG
  GCGTCCGACGGGCTGGACCTGAGCCAGGCGGCGGCGCGCACCAACGAGTACAAGATCATCCGCACCAACG
  AGAAGGAGCAGCTGCAGGGCCTCAACGACCGCTTCGCCGTGTTCATCGAGAAGGTGCATCAGCTGGAGAC
  GCAGAACCGCGCGTTGGAGGCCGAGCTGGCCGCGCTGCGACAGCGCCACGCTGAGCCGTCGCGCGTCGGC
  GAGCTCTTCCAGCGCGAGCTGCGCGACCTGCGCGCGCAGCTGGAGGAGGCCAGCTCGGCTCGCTCGCAGG
  CCCTGCTGGAGCGCGACGGGCTGGCGGAGGAGGTGCAGCGGCTGCGGGCGCGCTGCGAGGAGGAGAGCCG
  CGGACGCGAAGGCGCCGAGCGCGCCCTGAAGGCGCAGCAGCGCGACGTGGACGGCGCCACGCTGGCCCGC
  CTGGACCTGGAGAAGAAGGTGGAGTCGCTGCTGGACGAGCTGGCCTTCGTACGCCAGGTGCACGACGAGG
  AGGTAGCCGAGCTGCTGGCCACGCTGCAGGCGTCGTCGCAGGCCGCGGCCGAGGTGGACGTGACTGTGGC
  TAAACCAGACCTGACCTCGGCTCTGAGGGAGATCCGCGCCCAGTATGAGTCCCTGGCCGCTAAGAACCTG
  CAGTCCGCGGAAGAATGGTACAAGTCCAAGTTTGCCAACCTGAACGAGCAGGCGGCGCGCAGCACCGAGG
  CCATCCGGGCCAGCCGCGAGGAGATCCACGAGTATCGGCGCCAGCTGCAGGCGCGCACCATCGAGATCGA
  GGGCCTGCGCGGGGCCAACGAGTCCTTGGAGAGGCAGATCCTGGAGCTGGAGGAGCGGCACAGTGCCGAG
  GTAGCTGGCTACCAGGATAGCATTGGGCAGCTGGAGAATGATCTGAGGAACACCAAGAGTGAGATGGCAC
  GCCACCTTCGGGAATACCAGGACTTGCTCAATGTCAAAATGGCTCTTGACATTGAGATAGCAGCTTACAG
  GAAACTGCTGGAAGGCGAGGAGACACGTTTTAGCACCAGTGGGTTAAGCATTTCGGGGCTGAATCCACTT
  CCCAATCCAAGTTACCTGCTCCCACCTAGAATCCTCAGTGCTACAACCTCCAAAGTCTCATCCACTGGGC
  TATCACTTAAGAAAGAGGAGGAGGAGGAGGAGGCATCTAAGGTAGCCTCTAAGAAAACCTCCCAGATAGG
  GGAAAGTTTTGAAGAAATATTAGAGGAGACAGTAATATCTACTAAGAAAACCGAGAAATCAAATATAGAA
  GAAACCACCATTTCAAGCCAAAAAATA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG202877 representing NM_032727
  Red=Cloning site Green=Tags(s)
  
  MSFGSEHYLCSSSSYRKVFGDGSRLSARLSGAGGAGGFRSQSLSRSNVASSAACSSASSLGLGLAYRRPP
  ASDGLDLSQAAARTNEYKIIRTNEKEQLQGLNDRFAVFIEKVHQLETQNRALEAELAALRQRHAEPSRVG
  ELFQRELRDLRAQLEEASSARSQALLERDGLAEEVQRLRARCEEESRGREGAERALKAQQRDVDGATLAR
  LDLEKKVESLLDELAFVRQVHDEEVAELLATLQASSQAAAEVDVTVAKPDLTSALREIRAQYESLAAKNL
  QSAEEWYKSKFANLNEQAARSTEAIRASREEIHEYRRQLQARTIEIEGLRGANESLERQILELEERHSAE
  VAGYQDSIGQLENDLRNTKSEMARHLREYQDLLNVKMALDIEIAAYRKLLEGEETRFSTSGLSISGLNPL
  PNPSYLLPPRILSATTSKVSSTGLSLKKEEEEEEASKVASKKTSQIGESFEEILEETVISTKKTEKSNIE
  ETTISSQKI
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032727
• ORF Size: 1497 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_032727.2, NP_116116.1
• RefSeq Size: 3243
• RefSeq ORF: 1500
• Locus ID: 9118
• Domains: filament, filament_head
• Gene Summary: Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene is a member of the intermediate filament family and is involved in the morphogenesis of neurons. [provided by RefSeq, Jun 2009]