PLCD4 (NM_032726) Human Tagged ORF Clone

CAT#: RG202935

PLCD4 (GFP-tagged) - Human phospholipase C, delta 4 (PLCD4)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PLCD4
• Synonyms: MGC12837
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG202935 representing NM_032726
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCCCTGCTGCAAGACCAGCTGACCACTGATCAGGACTTGCTGCTGATGCAGGAAGGCATGCCGA
  TGCGCAAGGTGAGGTCCAAAAGCTGGAAGAAGCTAAGATACTTCAGACTTCAGAATGACGGCATGACAGT
  CTGGCATGCACGGCAGGCCAGGGGCAGTGCCAAGCCCAGCTTCTCAATCTCTGATGTGGAGACAATACGT
  AATGGCCATGATTCCGAGTTGCTGCGTAGCCTGGCAGAGGAGCTCCCCCTGGAGCAGGGCTTCACCATTG
  TCTTCCATGGCCGCCGCTCCAACCTGGACCTGATGGCCAACAGTGTTGAGGAGGCCCAGATATGGATGCG
  AGGGCTCCAGCTGTTGGTGGATCTTGTCACCAGCATGGACCATCAGGAGCGCCTGGACCAATGGCTGAGC
  GATTGGTTTCAACGTGGAGACAAAAATCAGGATGGTAAGATGAGTTTCCAAGAAGTTCAGCGGTTATTGC
  ACCTAATGAATGTGGAAATGGACCAAGAATATGCCTTCAGTCTTTTTCAGGCAGCAGACACGTCCCAGTC
  TGGAACCCTGGAAGGAGAAGAATTCGTACAGTTCTATAAGGCATTGACTAAACGTGCTGAGGTGCAGGAA
  CTGTTTGAAAGTTTTTCAGCTGATGGGCAGAAGCTGACTCTGCTGGAATTTTTGGATTTCCTCCAAGAGG
  AGCAGAAGGAGAGAGACTGCACCTCTGAGCTTGCTCTGGAACTCATTGACCGCTATGAACCTTCAGACAG
  TGGCAAACTGCGGCATGTGCTGAGTATGGATGGCTTCCTCAGCTACCTCTGCTCTAAGGATGGAGACATC
  TTCAACCCAGCCTGCCTCCCCATCTATCAGGATATGACTCAACCCCTGAACCACTACTTCATCTGCTCTT
  CTCATAACACCTACCTAGTGGGGGACCAGCTTTGCGGCCAGAGCAGCGTCGAGGGATATATACGGGCCCT
  GAAGCGGGGGTGCCGCTGCGTGGAGGTGGATGTATGGGATGGACCTAGCGGGGAACCTGTCGTTTACCAC
  GGACACACCCTGACCTCCCGCATCCTGTTCAAAGATGTCGTGGCCACAGTAGCACAGTATGCCTTCCAGA
  CATCAGACTACCCAGTCATCTTGTCCCTGGAGACCCACTGCAGCTGGGAGCAGCAGCAGACCATGGCCCG
  TCATCTGACTGAGATCCTGGGGGAGCAGCTGCTGAGCACCACCTTGGATGGGGTGCTGCCCACTCAGCTG
  CCCTCGCCTGAGGAGCTTCGGAGGAAGATCCTGGTGAAGGGGAAGAAGTTAACACTTGAGGAAGACCTGG
  AATATGAGGAAGAGGAAGCAGAACCTGAGTTGGAAGAGTCAGAATTGGCGCTGGAGTCCCAGTTTGAGAC
  TGAGCCTGAGCCCCAGGAGCAGAACCTTCAGAATAAGGACAAAAAGAAGAAATCCAAGCCCATCTTGTGT
  CCAGCCCTCTCTTCCCTGGTTATCTACTTGAAGTCTGTCTCATTCCGCAGCTTCACACATTCAAAGGAGC
  ACTACCACTTCTACGAGATATCATCTTTCTCTGAAACCAAGGCCAAGCGCCTCATCAAGGAGGCTGGCAA
  TGAGTTTGTGCAGCACAATACTTGGCAGTTAAGCCGTGTGTATCCCAGCGGCCTGAGGACAGACTCTTCC
  AACTACAACCCCCAGGAACTCTGGAATGCAGGCTGCCAGATGGTGGCCATGAATATGCAGACTGCAGGGC
  TTGAAATGGACATCTGTGATGGGCATTTCCGCCAGAATGGCGGCTGTGGCTATGTGCTGAAGCCAGACTT
  CCTGCGTGATATCCAGAGTTCTTTCCACCCTGAGAAGCCCATCAGCCCTTTCAAAGCCCAGACTCTCTTA
  ATCCAGGTGATCAGCGGTCAGCAACTCCCCAAAGTGGACAAGACCAAAGAGGGGTCCATTGTGGATCCAC
  TGGTGAAAGTGCAGATCTTTGGCGTTCGTCTAGACACAGCACGGCAGGAGACCAACTATGTGGAGAACAA
  TGGTTTTAATCCATACTGGGGGCAGACACTATGTTTCCGGGTGCTGGTGCCTGAACTTGCCATGCTGCGT
  TTTGTGGTAATGGATTATGACTGGAAATCCCGAAATGACTTTATTGGTCAGTACACCCTGCCTTGGACCT
  GCATGCAACAAGGTTACCGCCACATTCACCTGCTGTCCAAAGATGGCATCAGCCTCCGCCCAGCTTCCAT
  CTTTGTGTATATCTGCATCCAGGAAGGCCTGGAGGGGGATGAGTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG202935 representing NM_032726
  Red=Cloning site Green=Tags(s)
  
  MASLLQDQLTTDQDLLLMQEGMPMRKVRSKSWKKLRYFRLQNDGMTVWHARQARGSAKPSFSISDVETIR
  NGHDSELLRSLAEELPLEQGFTIVFHGRRSNLDLMANSVEEAQIWMRGLQLLVDLVTSMDHQERLDQWLS
  DWFQRGDKNQDGKMSFQEVQRLLHLMNVEMDQEYAFSLFQAADTSQSGTLEGEEFVQFYKALTKRAEVQE
  LFESFSADGQKLTLLEFLDFLQEEQKERDCTSELALELIDRYEPSDSGKLRHVLSMDGFLSYLCSKDGDI
  FNPACLPIYQDMTQPLNHYFICSSHNTYLVGDQLCGQSSVEGYIRALKRGCRCVEVDVWDGPSGEPVVYH
  GHTLTSRILFKDVVATVAQYAFQTSDYPVILSLETHCSWEQQQTMARHLTEILGEQLLSTTLDGVLPTQL
  PSPEELRRKILVKGKKLTLEEDLEYEEEEAEPELEESELALESQFETEPEPQEQNLQNKDKKKKSKPILC
  PALSSLVIYLKSVSFRSFTHSKEHYHFYEISSFSETKAKRLIKEAGNEFVQHNTWQLSRVYPSGLRTDSS
  NYNPQELWNAGCQMVAMNMQTAGLEMDICDGHFRQNGGCGYVLKPDFLRDIQSSFHPEKPISPFKAQTLL
  IQVISGQQLPKVDKTKEGSIVDPLVKVQIFGVRLDTARQETNYVENNGFNPYWGQTLCFRVLVPELAMLR
  FVVMDYDWKSRNDFIGQYTLPWTCMQQGYRHIHLLSKDGISLRPASIFVYICIQEGLEGDES
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032726
• ORF Size: 2286 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_032726.2, NP_116115.1
• RefSeq Size: 3107
• RefSeq ORF: 2289
• Locus ID: 84812
• Domains: C2, PI-PLC-X, PI-PLC-Y, PH, EFh
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• Gene Summary: This gene encodes a member of the delta class of phospholipase C enzymes. Phospholipase C enzymes play a critical role in many cellular processes by hydrolyzing phosphatidylinositol 4,5-bisphosphate into two intracellular second messengers, inositol 1,4,5-trisphosphate and diacylglycerol. Expression of this gene may be a marker for cancer. [provided by RefSeq, Jan 2011]