CHST11 (NM_018413) Human Tagged ORF Clone

CAT#: RG203037

CHST11 (GFP-tagged) - Human carbohydrate (chondroitin 4) sulfotransferase 11 (CHST11), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CHST11
• Synonyms: C4ST; C4ST-1; C4ST1; HSA269537; OCBMD
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203037 representing NM_018413
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCCAGCGCTGCTGGAAGTGATGAGGATGAACAGAATCTGCCGGATGGTGCTGGCCACTTGCTTGG
  GATCCTTTATCCTGGTCATCTTCTATTTCCAAAGTATGTTGCACCCAGTCATGCGGAGGAATCCCTTTGG
  TGTGGACATCTGCTGCCGGAAGGGGTCCCGAAGCCCCCTGCAGGAACTCTACAACCCAATCCAGCTGGAG
  CTCTCAAACACTGCTGTCCTGCACCAGATGCGGCGGGACCAGGTGACAGACACGTGCCGAGCCAACAGCG
  CCACAAGCCGTAAGCGGAGGGTGCTGACCCCCAACGACCTGAAGCACTTGGTGGTGGATGAGGACCACGA
  GCTCATCTACTGCTACGTGCCCAAGGTGGCCTGCACCAACTGGAAGCGGCTCATGATGGTCCTGACCGGG
  CGGGGGAAGTACAGCGACCCCATGGAGATCCCGGCCAACGAGGCACACGTCTCCGCCAACCTGAAGACCC
  TGAACCAGTACAGCATCCCAGAAATCAACCACCGCTTGAAAAGCTACATGAAGTTCCTGTTTGTCCGGGA
  GCCCTTCGAGAGGCTAGTGTCCGCCTACCGCAACAAGTTCACCCAGAAGTACAACATCTCCTTCCACAAG
  CGGTACGGCACCAAGATCATCAAACGCCAGCGGAAGAACGCCACCCAGGAGGCCCTGCGCAAAGGGGACG
  ATGTCAAATTCGAGGAGTTTGTGGCCTATCTCATCGACCCACACACCCAGCGGGAGGAGCCTTTCAACGA
  ACACTGGCAAACCGTCTACTCACTCTGCCATCCCTGCCACATCCACTATGACCTCGTGGGCAAGTACGAG
  ACACTGGAAGAGGATTCTAATTACGTCCTGCAGCTGGCAGGAGTGGGCAGCTACCTGAAGTTCCCCACCT
  ATGCAAAGTCTACGAGAACTACTGATGAAATGACCACAGAATTCTTCCAGAACATCAGCTCAGAGCACCA
  AACGCAGCTGTACGAAGTCTACAAACTCGATTTTTTAATGTTCAATTACTCAGTGCCAAGCTACCTGAAA
  TTGGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203037 representing NM_018413
  Red=Cloning site Green=Tags(s)
  
  MKPALLEVMRMNRICRMVLATCLGSFILVIFYFQSMLHPVMRRNPFGVDICCRKGSRSPLQELYNPIQLE
  LSNTAVLHQMRRDQVTDTCRANSATSRKRRVLTPNDLKHLVVDEDHELIYCYVPKVACTNWKRLMMVLTG
  RGKYSDPMEIPANEAHVSANLKTLNQYSIPEINHRLKSYMKFLFVREPFERLVSAYRNKFTQKYNISFHK
  RYGTKIIKRQRKNATQEALRKGDDVKFEEFVAYLIDPHTQREEPFNEHWQTVYSLCHPCHIHYDLVGKYE
  TLEEDSNYVLQLAGVGSYLKFPTYAKSTRTTDEMTTEFFQNISSEHQTQLYEVYKLDFLMFNYSVPSYLK
  LE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018413
• ORF Size: 1056 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_018413.2, NP_060883.1
• RefSeq Size: 1687
• RefSeq ORF: 1059
• Locus ID: 50515
• Domains: Sulfotransfer2
• Protein Families: Transmembrane
• Protein Pathways: Chondroitin sulfate biosynthesis, Sulfur metabolism
• Gene Summary: The protein encoded by this gene belongs to the sulfotransferase 2 family. It is localized to the golgi membrane, and catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage, and is distributed on the surfaces of many cells and extracellular matrices. A chromosomal translocation involving this gene and IgH, t(12;14)(q23;q32), has been reported in a patient with B-cell chronic lymphocytic leukemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]