SCLY (NM_016510) Human Tagged ORF Clone

CAT#: RG203164

SCLY (GFP-tagged) - Human selenocysteine lyase (SCLY)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SCLY
• Synonyms: hSCL; SCL
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203164 representing NM_016510
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCGGCCGTGGCGCCGGGGAGGGATGCGCCGGCACCCGCGGCGAGTCAGCCCAGCGGCTGCGGGA
  AACACAACTCGCCGGAGAGGAAAGTTTATATGGACTATAATGCAACGACTCCCCTGGAGCCAGAAGTTAT
  CCAGGCCATGACCAAGGCCATGTGGGAAGCCTGGGGAAATCCCAGCAGCCCGTATTCAGCAGGAAGAAAG
  GCCAAGGATATTATAAATGCAGCTCGGGAAAGCCTCGCGAAGATGATAGGGGGGAAACCTCAAGATATAA
  TCTTCACTTCCGGGGGCACTGAGTCAAATAATTTAGTAATCCATTCTGTGGTGAAACATTTCCACGCAAA
  CCAGACCTCAAAGGGACACACAGGTGGGCACCACAGCCCAGTGAAGGGGGCCAAGCCCCATTTCATTACT
  TCCTCGGTGGAACACGACTCCATCCGGCTGCCCCTGGAGCACCTGGTGGAAGAACAAGTGGCAGCGGTCA
  CCTTTGTCCCGGTGTCCAAGGTGAGCGGGCAGACAGAGGTGGACGACATCCTCGCGGCAGTCCGCCCGAC
  CACACGCCTCGTGACCATCATGCTGGCCAACAATGAGACTGGCATTGTCATGCCTGTCCCTGAAATCAGT
  CAGCGCATTAAAGCCCTGAACCAGGAACGGGTGGCAGCTGGGCTACCTCCCATCCTCGTGCACACGGATG
  CTGCACAGGCCTTGGGGAAGCAGCGCGTGGATGTGGAGGACCTGGGCGTGGACTTCCTTACAATCGTGGG
  GCACAAGTTTTATGGTCCCAGGATTGGCGCACTTTATATACGAGGACTTGGTGAATTTACCCCTCTCTAC
  CCTATGCTATTTGGAGGTGGACAAGAACGGAATTTCAGGCCAGGGACAGAGAACACCCCAATGATTGCTG
  GCCTTGGGAAGGCCGCGGAGCTGGTGACCCAGAACTGCGAGGCTTATGAGGCCCACATGAGGGACGTCCG
  CGACTACCTGGAAGAGAGGCTGGAAGCTGAATTCGGTCAGAAGAGAATCCATCTGAATAGCCAGTTTCCA
  GGCACCCAGCGGCTTCCCAATACCTGTAACTTTTCCATCCGGGGACCCCGGCTTCAAGGCCACGTGGTGC
  TTGCGCAGTGCCGAGTGCTGATGGCCAGTGTGGGGGCCGCGTGCCACTCGGACCACGGGGACCAGCCGTC
  CCCAGTGCTGCTGAGCTACGGTGTCCCCTTCGACGTGGCCAGGAACGCGCTCCGGCTCAGCGTGGGCCGC
  AGCACCACCAGGGCCGAGGTGGACCTCGTCGTGCAGGACCTGAAGCAGGCCGTGGCGCAGCTGGAGGACC
  AGGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203164 representing NM_016510
  Red=Cloning site Green=Tags(s)
  
  MEAAVAPGRDAPAPAASQPSGCGKHNSPERKVYMDYNATTPLEPEVIQAMTKAMWEAWGNPSSPYSAGRK
  AKDIINAARESLAKMIGGKPQDIIFTSGGTESNNLVIHSVVKHFHANQTSKGHTGGHHSPVKGAKPHFIT
  SSVEHDSIRLPLEHLVEEQVAAVTFVPVSKVSGQTEVDDILAAVRPTTRLVTIMLANNETGIVMPVPEIS
  QRIKALNQERVAAGLPPILVHTDAAQALGKQRVDVEDLGVDFLTIVGHKFYGPRIGALYIRGLGEFTPLY
  PMLFGGGQERNFRPGTENTPMIAGLGKAAELVTQNCEAYEAHMRDVRDYLEERLEAEFGQKRIHLNSQFP
  GTQRLPNTCNFSIRGPRLQGHVVLAQCRVLMASVGAACHSDHGDQPSPVLLSYGVPFDVARNALRLSVGR
  STTRAEVDLVVQDLKQAVAQLEDQA
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016510
• ORF Size: 1335 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_016510.3, NP_057594.2
• RefSeq Size: 2496
• RefSeq ORF: 1338
• Locus ID: 51540
• Domains: aminotran_5
• Protein Pathways: Metabolic pathways, Selenoamino acid metabolism
• Gene Summary: Selenocysteine lyase (SCLY; EC 4.4.1.16) catalyzes the pyridoxal 5-prime phosphate-dependent conversion of L-selenocysteine to L-alanine and elemental selenium (Mihara et al., 2000 [PubMed 10692412]). [supplied by OMIM, Mar 2008]