NARS2 (NM_024678) Human Tagged ORF Clone

CAT#: RG203201

NARS2 (GFP-tagged) - Human asparaginyl-tRNA synthetase 2, mitochondrial (putative) (NARS2), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NARS2
• Synonyms: asnRS; DFNB94; SLM5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203201 representing NM_024678
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGGGGTCCGCTGCCTGCTGCGGTCCGTGCGCTTCTGTTCCTCCGCCCCCTTCCCCAAGCACAAAC
  CTTCAGCCAAACTGAGCGTGCGGGACGCTCTCGGGGCTCAGAACGCGAGTGGGGAGCGCATTAAGATCCA
  GGGATGGATTCGTTCTGTCCGATCCCAGAAGGAAGTCTTGTTCCTGCATGTAAATGATGGGTCATCTTTG
  GAAAGCCTTCAGGTTGTTGCAGATTCAGGCCTTGACAGTAGAGAATTAACTTTTGGGAGTTCTGTGGAAG
  TACAAGGGCAGCTGATAAAAAGTCCATCCAAAAGGCAAAATGTGGAACTGAAGGCAGAAAAAATTAAAGT
  TATTGGAAATTGTGATGCCAAGGATTTCCCCATCAAATATAAAGAGAGGCATCCTCTGGAGTACCTGCGA
  CAATATCCTCACTTTAGGTGTAGGACTAACGTTCTGGGTTCTATATTGAGGATTCGCAGTGAAGCGACAG
  CTGCTATTCATTCTTTCTTTAAGGACAGTGGCTTTGTACATATTCATACTCCAATAATCACATCCAATGA
  CTCTGAGGGAGCTGGAGAACTTTTTCAACTTGAACCTTCAGGCAAACTTAAGGTACCTGAGGAGAATTTC
  TTCAATGTTCCTGCTTTCTTAACTGTCTCAGGACAACTTCATCTAGAAGTGATGTCAGGAGCTTTTACTC
  AAGTGTTTACCTTTGGTCCGACCTTCCGAGCTGAAAATTCTCAGAGCCGGAGGCACCTGGCAGAGTTTTA
  TATGATAGAAGCAGAGATTTCTTTTGTTGACAGCCTTCAAGATCTTATGCAGGTTATAGAGGAACTGTTC
  AAGGCTACAACAATGATGGTTCTCTCAAAATGTCCTGAAGATGTTGAACTCTGTCACAAATTCATAGCAC
  CTGGCCAAAAGGACAGATTAGAACATATGCTAAAAAACAACTTTTTAATCATTTCTTATACTGAAGCAGT
  GGAGATCTTAAAGCAAGCATCCCAGAACTTCACCTTTACCCCAGAGTGGGGTGCTGACCTACGGACTGAA
  CATGAAAAGTACCTGGTGAAGCACTGTGGCAACATACCTGTCTTCGTTATTAATTATCCATTAACACTCA
  AGCCTTTCTACATGAGGGATAATGAAGATGGCCCTCAGCACACGGTTGCTGCTGTTGATCTTCTGGTTCC
  TGGAGTTGGGGAACTCTTTGGAGGAGGCCTCAGAGAAGAACGATACCATTTCTTAGAGGAGCGCTTAGCC
  AGATCGGGACTTACAGAAGTCTACCAATGGTATCTGGACCTTCGTCGATTTGGATCTGTGCCACATGGAG
  GTTTTGGGATGGGATTTGAACGCTACCTGCAGTGCATCTTGGGTGTTGACAATATCAAAGATGTTATCCC
  TTTCCCAAGGTTTCCTCATTCATGCCTTTTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203201 representing NM_024678
  Red=Cloning site Green=Tags(s)
  
  MLGVRCLLRSVRFCSSAPFPKHKPSAKLSVRDALGAQNASGERIKIQGWIRSVRSQKEVLFLHVNDGSSL
  ESLQVVADSGLDSRELTFGSSVEVQGQLIKSPSKRQNVELKAEKIKVIGNCDAKDFPIKYKERHPLEYLR
  QYPHFRCRTNVLGSILRIRSEATAAIHSFFKDSGFVHIHTPIITSNDSEGAGELFQLEPSGKLKVPEENF
  FNVPAFLTVSGQLHLEVMSGAFTQVFTFGPTFRAENSQSRRHLAEFYMIEAEISFVDSLQDLMQVIEELF
  KATTMMVLSKCPEDVELCHKFIAPGQKDRLEHMLKNNFLIISYTEAVEILKQASQNFTFTPEWGADLRTE
  HEKYLVKHCGNIPVFVINYPLTLKPFYMRDNEDGPQHTVAAVDLLVPGVGELFGGGLREERYHFLEERLA
  RSGLTEVYQWYLDLRRFGSVPHGGFGMGFERYLQCILGVDNIKDVIPFPRFPHSCLL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024678
• ORF Size: 1431 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_024678.3, NP_078954.3
• RefSeq Size: 2110
• RefSeq ORF: 1434
• Locus ID: 79731
• Domains: tRNA-synt_2, tRNA_anti
• Protein Pathways: Aminoacyl-tRNA biosynthesis
• Gene Summary: This gene encodes a putative member of the class II family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. This protein is encoded by the nuclear genome but is likely to be imported to the mitochondrion where it is thought to catalyze the ligation of asparagine to tRNA molecules. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency 24 (COXPD24). [provided by RefSeq, Mar 2015]