CAPNS1 (NM_001003962) Human Tagged ORF Clone
CAT#: RG203233
- TrueORF®
CAPNS1 (GFP-tagged) - Human calpain, small subunit 1 (CAPNS1), transcript variant 2
"NM_001003962" in other vectors (4)
Product Images
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | CAPNS1 |
Synonyms | CALPAIN4; CANP; CANPS; CAPN4; CDPS; CSS1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG203233 representing NM_001003962
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCTGGTTAACTCGTTCTTGAAGGGCGGCGGCGGCGGCGGCGGGGGAGGCGGGGGCCTGGGTGGGG GCCTGGGAAATGTGCTTGGAGGCCTGATCAGCGGGGCCGGGGGCGGCGGCGGCGGCGGCGGCGGCGGCGG CGGTGGTGGAGGCGGCGGTGGCGGTGGAACGGCCATGCGCATCCTAGGCGGAGTCATCAGCGCCATCAGC GAGGCGGCTGCGCAGTACAACCCGGAGCCCCCGCCCCCACGCACACATTACTCCAACATTGAGGCCAACG AGAGTGAGGAGGTCCGGCAGTTCCGGAGACTCTTTGCCCAGCTGGCTGGAGATGACATGGAGGTCAGCGC CACAGAACTCATGAACATTCTCAATAAGGTTGTGACACGACACCCTGATCTGAAGACTGATGGTTTTGGC ATTGACACATGTCGCAGCATGGTGGCCGTGATGGATAGCGACACCACAGGCAAGCTGGGCTTTGAGGAAT TCAAGTACTTGTGGAACAACATCAAAAGGTGGCAGGCCATATACAAACAGTTCGACACTGACCGATCAGG GACCATTTGCAGTAGTGAACTCCCAGGTGCCTTTGAGGCAGCAGGGTTCCACCTGAATGAGCATCTCTAT AACATGATCATCCGACGCTACTCAGATGAAAGTGGGAACATGGATTTTGACAACTTCATCAGCTGCTTGG TCAGGCTGGACGCCATGTTCCGTGCCTTCAAATCTCTTGACAAAGATGGCACTGGACAAATCCAGGTGAA CATCCAGGAGTGGCTGCAGCTGACTATGTATTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG203233 representing NM_001003962
Red=Cloning site Green=Tags(s) MFLVNSFLKGGGGGGGGGGGLGGGLGNVLGGLISGAGGGGGGGGGGGGGGGGGGGGTAMRILGGVISAIS EAAAQYNPEPPPPRTHYSNIEANESEEVRQFRRLFAQLAGDDMEVSATELMNILNKVVTRHPDLKTDGFG IDTCRSMVAVMDSDTTGKLGFEEFKYLWNNIKRWQAIYKQFDTDRSGTICSSELPGAFEAAGFHLNEHLY NMIIRRYSDESGNMDFDNFISCLVRLDAMFRAFKSLDKDGTGQIQVNIQEWLQLTMYS TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001003962 |
ORF Size | 804 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001003962.1, NP_001003962.1 |
RefSeq Size | 1489 bp |
RefSeq ORF | 807 bp |
Locus ID | 826 |
Cytogenetics | 19q13.12 |
Protein Families | Druggable Genome, Protease |
Gene Summary | 'This gene is a member of the calpain small subunit family. Calpains are calcium-dependent cysteine proteinases that are widely distributed in mammalian cells. Calpains operate as heterodimers, comprising a specific large catalytic subunit (calpain 1 subunit in Calpain I, and calpain 2 subunit in Calpain II), and a common small regulatory subunit encoded by this gene. This encoded protein is essential for the stability and function of both calpain heterodimers, whose proteolytic activities influence various cellular functions including apoptosis, proliferation, migration, adhesion, and autophagy. Calpains have been implicated in neurodegenerative processes, such as myotonic dystrophy. A pseudogene of this gene has been defined on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC300585 | CAPNS1 (untagged)-Human calpain, small subunit 1 (CAPNS1), transcript variant 2 |
USD 660.00 |
|
RC203233 | CAPNS1 (Myc-DDK-tagged)-Human calpain, small subunit 1 (CAPNS1), transcript variant 2 |
USD 420.00 |
|
RC203233L3 | Lenti-ORF clone of CAPNS1 (Myc-DDK-tagged)-Human calpain, small subunit 1 (CAPNS1), transcript variant 2 |
USD 620.00 |
|
RC203233L4 | Lenti-ORF clone of CAPNS1 (mGFP-tagged)-Human calpain, small subunit 1 (CAPNS1), transcript variant 2 |
USD 620.00 |
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