DTX2 (NM_020892) Human Tagged ORF Clone

CAT#: RG203717

DTX2 (GFP-tagged) - Human deltex homolog 2 (Drosophila) (DTX2), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DTX2
• Synonyms: RNF58
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203717 representing NM_020892
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCATGGCCCCAAGCCCTTCCCTGGTGCAGGTGTACACCAGCCCCGCGGCTGTGGCCGTGTGGGAAT
  GGCAGGACGGGCTGGGCACCTGGCACCCCTACAGTGCCACCGTCTGCAGCTTCATCGAGCAGCAGTTTGT
  CCAGCAGAAGGGCCAACGTTTTGGGCTTGGGAGCCTGGCCCACAGCATCCCCTTGGGCCAGGCAGACCCC
  TCGCTGGCCCCTTACATTATTGACCTCCCCAGCTGGACCCAGTTCCGCCAGGACACCGGCACCATGCGGG
  CTGTGCGGAGACACCTGTTCCCCCAGCACTCAGCCCCTGGCCGAGGTGTCGTCTGGGAGTGGCTGAGCGA
  CGATGGCTCCTGGACTGCCTATGAAGCCAGCGTCTGTGACTATCTGGAGCAGCAGGTGGCCAGGGGCAAC
  CAGCTCGTGGACTTGGCCCCCCTGGGGTACAACTACACTGTCAACTACACCACCCACACGCAGACCAACA
  AGACTTCCAGCTTTTGCCGCAGCGTGCGGCGCCAAGCAGGGCCGCCTTACCCGGTGACCACCATCATCGC
  TCCGCCGGGCCACACAGGCGTCGCCTGCTCTTGCCACCAGTGCCTCAGTGGCAGCAGAACTGGCCCCGTG
  TCAGGCCGCTACCGCCACTCCATGACCAACCTCCCTGCATACCCCGTCCCCCAGCACCCCCCACACAGGA
  CCGCTTCTGTGTTTGGGACCCACCAGGCCTTTGCACCGTACAACAAACCCTCACTCTCCGGGGCCCGGTC
  TGCGCCCAGGCTGAACACCACCAACGCCTGGGGCGCAGCTCCTCCTTCCCTGGGGAGCCAGCCCCTCTAC
  CGCTCCAGCCTCTCCCACCTGGGACCGCAGCACCTGCCCCCAGGATCCTCCACCTCCGGTGCAGTCAGTG
  CCTCCCTCCCCAGCGGTCCCTCAAGCAGCCCAGGGAGCGTCCCTGCCACTGTGCCCATGCAGATGCCAAA
  GCCCAGCAGAGTCCAGCAGGCGCTCGCAGGCATGACGAGTGTTCTGATGTCAGCCATTGGACTCCCTGTG
  TGTCTTAGCCGCGCACCCCAGCCCACCAGCCCTCCCGCCTCCCGTCTGGCTTCCAAAAGTCACGGCTCAG
  TTAAGAGATTGAGGAAAATGTCCGTGAAAGGAGCGACCCCGAAGCCAGAGCCAGAGCCAGAGCAGGTCAT
  AAAAAACTACACGGAAGAGCTGAAAGTGCCCCCAGATGAGGACTGCATCATCTGCATGGAGAAGCTGTCC
  GCAGCGTCTGGATACAGCGATGTGACTGACAGCAAGGCAATCGGGTCCCTAGCTGTGGGCCACCTCACCA
  AGTGCAGCCATGCCTTCCACCTGCTGTGCCTCCTGGCCATGTACTGCAACGGCAATAAGGATGGAAGTCT
  GCAGTGTCCCTCCTGCAAAACCATCTATGGAGAGAAGACGGGGACCCAGCCCCAGGGAAAGATGGAGGTA
  TTACGGTTCCAGATGTCGCTCCCCGGCCACGAGGACTGCGGGACCATCCTCATAGTTTACAGCATTCCCC
  ATGGTATCCAGGGCCCTGAGCACCCCAATCCCGGAAAGCCGTTCACTGCCAGAGGGTTTCCCCGCCAGTG
  CTACCTTCCAGACAACGCCCAGGGCCGCAAGGTCCTAGAGCTCCTGAAGGTGGCCTGGAAGAGGCGGCTC
  ATCTTCACAGTGGGCACGTCCAGCACCACGGGTGAGACGGACACCGTGGTATGGAACGAGATCCACCACA
  AGACAGAGATGGACCGCAACATTACGGGCCACGGCTATCCCGACCCCAACTACCTGCAGAACGTGCTGGC
  TGAGCTGGCTGCCCAGGGGGTGACCGAGGACTGCCTGGAGCAGCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203717 representing NM_020892
  Red=Cloning site Green=Tags(s)
  
  MAMAPSPSLVQVYTSPAAVAVWEWQDGLGTWHPYSATVCSFIEQQFVQQKGQRFGLGSLAHSIPLGQADP
  SLAPYIIDLPSWTQFRQDTGTMRAVRRHLFPQHSAPGRGVVWEWLSDDGSWTAYEASVCDYLEQQVARGN
  QLVDLAPLGYNYTVNYTTHTQTNKTSSFCRSVRRQAGPPYPVTTIIAPPGHTGVACSCHQCLSGSRTGPV
  SGRYRHSMTNLPAYPVPQHPPHRTASVFGTHQAFAPYNKPSLSGARSAPRLNTTNAWGAAPPSLGSQPLY
  RSSLSHLGPQHLPPGSSTSGAVSASLPSGPSSSPGSVPATVPMQMPKPSRVQQALAGMTSVLMSAIGLPV
  CLSRAPQPTSPPASRLASKSHGSVKRLRKMSVKGATPKPEPEPEQVIKNYTEELKVPPDEDCIICMEKLS
  AASGYSDVTDSKAIGSLAVGHLTKCSHAFHLLCLLAMYCNGNKDGSLQCPSCKTIYGEKTGTQPQGKMEV
  LRFQMSLPGHEDCGTILIVYSIPHGIQGPEHPNPGKPFTARGFPRQCYLPDNAQGRKVLELLKVAWKRRL
  IFTVGTSSTTGETDTVVWNEIHHKTEMDRNITGHGYPDPNYLQNVLAELAAQGVTEDCLEQQ
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020892
• ORF Size: 1866 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_020892.1, NP_065943.1
• RefSeq Size: 2782
• RefSeq ORF: 1869
• Locus ID: 113878
• Domains: RING, WWE
• Protein Families: Druggable Genome
• Protein Pathways: Notch signaling pathway
• Gene Summary: DTX2 functions as an E3 ubiquitin ligase (Takeyama et al., 2003 [PubMed 12670957]). [supplied by OMIM, Nov 2009]