ZNF35 (NM_003420) Human Tagged ORF Clone

CAT#: RG203770

ZNF35 (GFP-tagged) - Human zinc finger protein 35 (ZNF35)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ZNF35
• Synonyms: HF.10; HF10; Zfp105
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203770 representing NM_003420
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGCAGAATTGAGAGAAGCCATGGCCCTAGCCCCATGGGGCCCAGTGAAGGTGAAAAAGGAGGAGG
  AAGAAGAAGAAAACTTCCCAGGTCAGGCATCCAGCCAACAAGTGCACTCCGAGAACATCAAAGTCTGGGC
  CCCAGTGCAGGGTCTTCAGACAGGCCTTGATGGATCAGAAGAGGAAGAAAAGGGTCAGAACATATCCTGG
  GATATGGCGGTAGTCCTGAAAGCAACTCAGGAGGCACCTGCTGCTTCAACCCTTGGCAGCTACTCATTAC
  CAGGGACTCTGGCCAAGAGTGAGATACTGGAGACTCATGGGACCATGAACTTTCTAGGTGCTGAAACCAA
  GAACCTACAGTTACTGGTTCCAAAAACTGAGATATGTGAGGAAGCTGAAAAACCCCTCATCATATCAGAA
  AGAATCCAGAAAGCTGATCCTCAAGGACCTGAGTTAGGAGAAGCTTGTGAAAAGGGAAACATGTTAAAGA
  GGCAGAGAATAAAGAGAGAAAAGAAAGATTTCAGACAAGTGATAGTGAATGACTGTCACTTACCTGAAAG
  CTTCAAAGAAGAGGAAAACCAGAAATGTAAGAAATCTGGAGGAAAATATAGCCTTAATTCTGGCGCTGTT
  AAAAATCCAAAAACCCAGCTTGGACAAAAGCCTTTTACGTGTAGCGTGTGTGGGAAAGGATTTAGTCAGA
  GTGCAAACCTCGTTGTGCATCAGCGAATCCACACTGGAGAGAAACCCTTTGAATGTCATGAGTGTGGGAA
  GGCCTTCATTCAGAGTGCAAACCTCGTTGTGCATCAGAGAATCCACACTGGACAGAAACCTTATGTTTGC
  TCAAAATGTGGGAAAGCCTTCACTCAGAGTTCAAATCTGACTGTACATCAAAAAATCCACTCCTTAGAAA
  AAACTTTTAAGTGCAATGAATGTGAGAAAGCCTTTAGTTACAGCTCACAACTTGCTCGGCACCAGAAAGT
  CCACATTACGGAAAAATGCTATGAATGTAATGAATGTGGGAAAACATTTACTAGGAGCTCAAACCTCATT
  GTCCACCAGAGGATCCACACTGGGGAGAAGCCCTTTGCCTGTAACGACTGTGGCAAAGCCTTTACCCAGA
  GTGCAAATCTTATTGTACATCAGCGAAGCCATACTGGTGAGAAGCCATATGAGTGTAAAGAGTGTGGGAA
  AGCCTTTAGTTGTTTTTCACACCTTATTGTGCACCAGAGAATTCACACTGCAGAGAAACCTTACGACTGC
  AGCGAATGTGGGAAAGCCTTCAGTCAGCTCTCTTGCCTTATTGTCCACCAGAGAATTCACAGTGGAGATC
  TTCCTTACGTGTGTAATGAATGTGGGAAGGCCTTCACATGTAGCTCATACCTACTTATTCATCAGAGAAT
  TCATAATGGAGAAAAACCTTACACATGTAATGAGTGTGGGAAGGCCTTCAGACAGAGGTCGAGCCTCACC
  GTGCACCAGAGAACCCACACTGGGGAGAAGCCCTATGAATGTGAGAAGTGTGGTGCAGCTTTCATTTCCA
  ACTCACACCTCATGCGACACCATAGAACCCATCTTGTTGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203770 representing NM_003420
  Red=Cloning site Green=Tags(s)
  
  MTAELREAMALAPWGPVKVKKEEEEEENFPGQASSQQVHSENIKVWAPVQGLQTGLDGSEEEEKGQNISW
  DMAVVLKATQEAPAASTLGSYSLPGTLAKSEILETHGTMNFLGAETKNLQLLVPKTEICEEAEKPLIISE
  RIQKADPQGPELGEACEKGNMLKRQRIKREKKDFRQVIVNDCHLPESFKEEENQKCKKSGGKYSLNSGAV
  KNPKTQLGQKPFTCSVCGKGFSQSANLVVHQRIHTGEKPFECHECGKAFIQSANLVVHQRIHTGQKPYVC
  SKCGKAFTQSSNLTVHQKIHSLEKTFKCNECEKAFSYSSQLARHQKVHITEKCYECNECGKTFTRSSNLI
  VHQRIHTGEKPFACNDCGKAFTQSANLIVHQRSHTGEKPYECKECGKAFSCFSHLIVHQRIHTAEKPYDC
  SECGKAFSQLSCLIVHQRIHSGDLPYVCNECGKAFTCSSYLLIHQRIHNGEKPYTCNECGKAFRQRSSLT
  VHQRTHTGEKPYECEKCGAAFISNSHLMRHHRTHLVE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003420
• ORF Size: 1581 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003420.3, NP_003411.3
• RefSeq Size: 2664 bp
• RefSeq ORF: 1584 bp
• Locus ID: 7584
• Cytogenetics: 3p21.31
• Domains: zf-C2H2
• Protein Families: Transcription Factors
• Gene Summary: ''