MRRF (NM_138777) Human Tagged ORF Clone

CAT#: RG203909

MRRF (GFP-tagged) - Human mitochondrial ribosome recycling factor (MRRF), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MRRF
• Synonyms: MRFF; MTRRF; RRF
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203909 representing NM_138777
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTTGGGATTAAAGTGCTTCCGCATGGTCCACCCTACCTTTCGCAATTATCTTGCAGCCTCTATCA
  GACCCGTTTCAGAAGTTACACTGAAGACAGTGCATGAAAGACAACATGGCCATAGGCAATACATGGCCTA
  TTCAGCTGTACCAGTCCGCCATTTTGCTACCAAGAAAGCCAAAGCCAAAGGGAAAGGACAGTCCCAAACC
  AGAGTGAATATTAATGCTGCCTTGGTTGAGGATATAATCAACTTGGAAGAGGTGAATGAAGAAATGAAGT
  CTGTGATAGAAGCTCTCAAGGATAATTTCAATAAGACTCTCAATATAAGGACCTCACCAGGATCCCTTGA
  CAAGATTGCTGTGGTAACTGCTGACGGGAAGCTTGCTTTAAACCAGATTAGCCAGATCTCCATGAAGTCG
  CCACAGCTGATTTTGGTGAATATGGCCAGCTTCCCAGAGTGTACAGCTGCAGCTATCAAGGCTATAAGAG
  AAAGTGGAATGAATCTGAACCCAGAAGTGGAAGGGACGCTAATTCGGGTACCCATTCCCCAAGTAACCAG
  AGAGCACAGAGAAATGCTGGTGAAACTGGCCAAACAGAACACCAACAAGGCCAAAGACTCTTTACGGAAG
  GTTCGCACCAACTCAATGAACAAGCTGAAGAAATCCAAGGATACAGTCTCAGAGGACACCATTAGGCTAA
  TAGAGAAACAGATCAGCCAAATGGCCGATGACACAGTGGCAGAACTGGACAGGCATCTGGCAGTGAAGAC
  CAAAGAACTCCTTGGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203909 representing NM_138777
  Red=Cloning site Green=Tags(s)
  
  MALGLKCFRMVHPTFRNYLAASIRPVSEVTLKTVHERQHGHRQYMAYSAVPVRHFATKKAKAKGKGQSQT
  RVNINAALVEDIINLEEVNEEMKSVIEALKDNFNKTLNIRTSPGSLDKIAVVTADGKLALNQISQISMKS
  PQLILVNMASFPECTAAAIKAIRESGMNLNPEVEGTLIRVPIPQVTREHREMLVKLAKQNTNKAKDSLRK
  VRTNSMNKLKKSKDTVSEDTIRLIEKQISQMADDTVAELDRHLAVKTKELLG
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138777
• ORF Size: 786 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_138777.2, NP_620132.1
• RefSeq Size: 1787
• RefSeq ORF: 789
• Locus ID: 92399
• Domains: RRF
• Protein Families: Transmembrane
• Gene Summary: This gene encodes a ribosome recycling factor, which is a component of the mitochondrial translational machinery. The encoded protein, along with mitochondrial elongation factor 2, functions in ribosomal recycling at the termination of mitochondrial translation by mediating the disassembly of ribosomes from messenger RNA. A pseudogene of this gene has been identified on chromosome X. [provided by RefSeq, Oct 2016]