MAGED1 (NM_001005333) Human Tagged ORF Clone

CAT#: RG204088

MAGED1 (GFP-tagged) - Human melanoma antigen family D, 1 (MAGED1), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MAGED1
• Synonyms: DLXIN-1; NRAGE
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204088 representing NM_001005333
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCAGAAAATGGACTGTGGTGCGGGCCTCCTCGGCTTCCAGAATCCTGACGCTTGTAGAGCAGTCT
  GTCACCCCCTCCCCCAGCCTCCTGCCTCCACCCTCCCTCTTTCTGCATTCCCCACTCTGTGCGACCCCCC
  TTATTCTCAACTCCGCGATCCGCCTGCCGTCCTGAGCTGCTACTGCACGCCTCTTGGAGCGTCCCCGGCT
  CCTGCTGAGGCCTCCGTAGAAGACAGCGCCTTGCTTATGCAGACCTTGATGGAGGCCATCCAGATCTCAG
  AGGCTCCACCTACTAACCAGGCCACCGCAGCTGCTAGTCCCCAGAGTTCACAGCCCCCAACTGCCAATGA
  GATGGCTGACATTCAGGTTTCAGCAGCTGCCGCTAGGCCTAAGTCAGCCTTTAAAGTCCAGAATGCCACC
  ACAAAAGGCCCAAATGGTGTCTATGATTTCTCTCAGGCTCATAATGCCAAGGATGTGCCCAACACGCAGC
  CCAAGGCAGCCTTTAAGTCCCAAAATGCTACCCCAAAGGGTCCAAATGCTGCCTATGATTTTTCCCAGGC
  AGCAACCACTGGTGAGTTAGCTGCTAACAAGTCTGAGATGGCCTTCAAGGCCCAGAATGCCACTACTAAA
  GTGGGCCCAAATGCCACCTACAATTTCTCTCAGTCTCTCAATGCCAATGACCTGGCCAACAGCAGGCCTA
  AGACCCCTTTCAAGGCTTGGAATGATACCACTAAGGCCCCAACAGCTGATACCCAGACCCAGAATGTAAA
  TCAGGCCAAAATGGCCACTTCCCAGGCTGACATAGAGACCGACCCAGGTATCTCTGAACCTGACGGTGCA
  ACTGCACAGACATCAGCAGATGGTTCCCAGGCTCAGAATCTGGAGTCCCGGACAATAATTCGGGGCAAGA
  GGACCCGCAAGATTAATAACTTGAATGTTGAAGAGAACAGCAGTGGGGATCAGAGGCGGGCCCCACTGGC
  TGCAGGGACCTGGAGGTCTGCACCAGTTCCAGTGACCACTCAGAACCCACCTGGCGCACCCCCCAATGTG
  CTCTGGCAGACGCCATTGGCTTGGCAGAACCCCTCAGGCTGGCAAAACCAGACAGCCAGGCAGACCCCAC
  CAGCACGTCAGAGCCCTCCAGCTAGGCAGACCCCACCAGCCTGGCAGAACCCAGTCGCTTGGCAGAACCC
  AGTGATTTGGCCAAACCCAGTAATCTGGCAGAACCCAGTGATCTGGCCAAACCCCATTGTCTGGCCCGGC
  CCTGTTGTCTGGCCGAATCCACTGGCCTGGCAGAATCCACCTGGATGGCAGACTCCACCTGGATGGCAGA
  CCCCACCGGGCTGGCAGGGTCCTCCAGACTGGCAAGGTCCTCCTGACTGGCCGCTACCACCCGACTGGCC
  ACTGCCACCTGATTGGCCACTTCCCACTGACTGGCCACTACCACCTGACTGGATCCCCGCTGATTGGCCA
  ATTCCACCTGACTGGCAGAACCTGCGCCCCTCGCCTAACCTGCGCCCTTCTCCCAACTCGCGTGCCTCAC
  AGAACCCAGGTGCTGCACAGCCCCGAGATGTGGCCCTTCTTCAGGAAAGAGCAAATAAGTTGGTCAAGTA
  CTTGATGCTTAAGGACTACACAAAGGTGCCCATCAAGCGCTCAGAAATGCTGAGAGATATCATCCGTGAA
  TACACTGATGTTTATCCAGAAATCATTGAACGTGCATGCTTTGTCCTAGAGAAGAAATTTGGGATTCAAC
  TGAAAGAAATTGACAAAGAAGAACACCTGTATATTCTCATCAGTACCCCCGAGTCCCTGGCTGGCATACT
  GGGAACGACCAAAGACACACCCAAGCTCGGTCTCCTCTTGGTGATTCTGGGTGTCATCTTCATGAATGGC
  AACCGTGCCAGTGAGGCTGTCCTCTGGGAGGCACTACGCAAGATGGGACTGCGTCCTGGGGTGAGACATC
  CCCTCCTTGGAGATCTAAGGAAACTTCTCACCTATGAGTTTGTAAAGCAGAAATACCTGGACTACAGACG
  AGTGCCCAACAGCAACCCCCCGGAGTATGAGTTCCTCTGGGGCCTCCGTTCCTACCATGAGACTAGCAAG
  ATGAAAGTGCTGAGATTCATTGCAGAGGTTCAGAAAAGAGACCCTCGTGACTGGACTGCACAGTTCATGG
  AGGCTGCAGATGAGGCCTTGGATGCTCTGGATGCTGCTGCAGCTGAGGCCGAAGCCCGGGCTGAAGCAAG
  AACCCGCATGGGAATTGGAGATGAGGCTGTGTCTGGGCCCTGGAGCTGGGATGACATTGAGTTTGAGCTG
  CTGACCTGGGATGAGGAAGGAGATTTTGGAGATCCCTGGTCCAGAATTCCATTTACCTTCTGGGCCAGAT
  ACCACCAGAATGCCCGCTCCAGATTCCCTCAGACCTTTGCCGGTCCCATTATTGGTCCTGGTGGTACAGC
  CAGTGCCAACTTCGCTGCCAACTTTGGTGCCATTGGTTTCTTCTGGGTTGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204088 representing NM_001005333
  Red=Cloning site Green=Tags(s)
  
  MAQKMDCGAGLLGFQNPDACRAVCHPLPQPPASTLPLSAFPTLCDPPYSQLRDPPAVLSCYCTPLGASPA
  PAEASVEDSALLMQTLMEAIQISEAPPTNQATAAASPQSSQPPTANEMADIQVSAAAARPKSAFKVQNAT
  TKGPNGVYDFSQAHNAKDVPNTQPKAAFKSQNATPKGPNAAYDFSQAATTGELAANKSEMAFKAQNATTK
  VGPNATYNFSQSLNANDLANSRPKTPFKAWNDTTKAPTADTQTQNVNQAKMATSQADIETDPGISEPDGA
  TAQTSADGSQAQNLESRTIIRGKRTRKINNLNVEENSSGDQRRAPLAAGTWRSAPVPVTTQNPPGAPPNV
  LWQTPLAWQNPSGWQNQTARQTPPARQSPPARQTPPAWQNPVAWQNPVIWPNPVIWQNPVIWPNPIVWPG
  PVVWPNPLAWQNPPGWQTPPGWQTPPGWQGPPDWQGPPDWPLPPDWPLPPDWPLPTDWPLPPDWIPADWP
  IPPDWQNLRPSPNLRPSPNSRASQNPGAAQPRDVALLQERANKLVKYLMLKDYTKVPIKRSEMLRDIIRE
  YTDVYPEIIERACFVLEKKFGIQLKEIDKEEHLYILISTPESLAGILGTTKDTPKLGLLLVILGVIFMNG
  NRASEAVLWEALRKMGLRPGVRHPLLGDLRKLLTYEFVKQKYLDYRRVPNSNPPEYEFLWGLRSYHETSK
  MKVLRFIAEVQKRDPRDWTAQFMEAADEALDALDAAAAEAEARAEARTRMGIGDEAVSGPWSWDDIEFEL
  LTWDEEGDFGDPWSRIPFTFWARYHQNARSRFPQTFAGPIIGPGGTASANFAANFGAIGFFWVE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001005333
• ORF Size: 2502 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001005333.1, NP_001005333.1
• RefSeq Size: 2928
• RefSeq ORF: 2505
• Locus ID: 9500
• Protein Families: Druggable Genome
• Protein Pathways: Neurotrophin signaling pathway
• Gene Summary: This gene is a member of the melanoma antigen gene (MAGE) family. Most of the genes of this family encode tumor specific antigens that are not expressed in normal adult tissues except testis. Although the protein encoded by this gene shares strong homology with members of the MAGE family, it is expressed in almost all normal adult tissues. This gene has been demonstrated to be involved in the p75 neurotrophin receptor mediated programmed cell death pathway. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]