POLR3K (NM_016310) Human Tagged ORF Clone

CAT#: RG204102

POLR3K (GFP-tagged) - Human polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa (POLR3K)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: POLR3K
• Synonyms: C11; C11-RNP3; My010; RPC10; RPC11; RPC12.5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204102 representing NM_016310
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCTGTTCTGCCCCGGCTGCGGGAACGGGCTGATCGTGGAGGAGGGACAACGCTGCCACCGCTTCG
  CCTGCAACACGTGCCCCTACGTGCACAACATCACCCGCAAGGTAACAAATCGGAAGTACCCAAAACTGAA
  AGAAGTGGATGATGTGCTTGGTGGAGCAGCTGCCTGGGAGAATGTTGACTCTACTGCAGAGTCGTGTCCC
  AAATGCGAACATCCTCGTGCTTACTTCATGCAGCTTCAGACCCGCTCTGCAGATGAGCCGATGACCACCT
  TCTACAAGTGCTGCAATGCTCAGTGTGGACACCGCTGGAGGGAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204102 representing NM_016310
  Red=Cloning site Green=Tags(s)
  
  MLLFCPGCGNGLIVEEGQRCHRFACNTCPYVHNITRKVTNRKYPKLKEVDDVLGGAAAWENVDSTAESCP
  KCEHPRAYFMQLQTRSADEPMTTFYKCCNAQCGHRWRD
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016310
• ORF Size: 324 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_016310.2, NP_057394.1
• RefSeq Size: 796
• RefSeq ORF: 327
• Locus ID: 51728
• Domains: TFIIS, RNA_POL_M_15KD
• Protein Families: Transcription Factors
• Protein Pathways: Cytosolic DNA-sensing pathway, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
• Gene Summary: This gene encodes a small essential subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The carboxy-terminal domain of this subunit shares a high degree of sequence similarity to the carboxy-terminal domain of an RNA polymerase II elongation factor. This similarity in sequence is supported by functional studies showing that this subunit is required for proper pausing and termination during transcription. Pseudogenes of this gene are found on chromosomes 13 and 17. [provided by RefSeq, Jul 2010]