RNMTL1 (MRM3) (NM_018146) Human Tagged ORF Clone

CAT#: RG204113

RNMTL1 (GFP-tagged) - Human RNA methyltransferase like 1 (RNMTL1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MRM3
• Synonyms: RMTL1; RNMTL1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204113 representing NM_018146
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGCTGGTGAGACCCTCGAGGTTTGTCGTGCGACCGTTGCTGCAGGTGGTCCAGGCTTGGGACC
  TTGACGCGAGGCGCTGGGTCCGGGCGCTGCGGCGGAGCCCAGTGAAAGTGGTGTTTCCTTCCGGAGAGGT
  GGTGGAACAGAAGCGCGCTCCTGGGAAGCAGCCCCGCAAGGCACCATCTGAGGCCAGTGCCCAGGAGCAA
  CGAGAGAAACAACCGCTCGAGGAGTCCGCATCCCGCGCTCCCAGCACCTGGGAAGAGTCTGGGCTTCGCT
  ACGATAAAGCTTATCCCGGGGACAGGAGGCTGAGCAGTGTAATGACAATAGTAAAGTCCAGGCCATTTCG
  GGAAAAACAAGGGAAGATCCTGCTGGAAGGTCGCAGGCTCATTTCAGACGCTCTCAAGGCTGGAGCTGTG
  CCAAAAATGTTCTTCTTTAGCCGTCTAGAATACCTAAAGGAGTTGCCAGTCGATAAGCTGAAAGGTGTCA
  GCCTCATTAAGGTGAAATTTGAGGATATCAAGGATTGGTCCGACCTCGTAACGCCACAAGGAATAATGGG
  GATTTTTGCCAAGCCTGACCATGTTAAGATGACATATCCAAAGACTCAGCTTCAGCATTCACTGCCTTTA
  TTATTGATTTGTGACAATCTCCGTGACCCTGGGAACCTGGGGACAATTCTGAGATCTGCAGCTGGGGCAG
  GCTGCAGCAAAGTGTTACTCACCAAAGGCTGTGTGGATGCCTGGGAGCCCAAAGTGCTCCGGGCGGGTAT
  GGGCGCACATTTCCGGATGCCCATTATCAATAATCTGGAATGGGAAACCGTGCCCAATTACCTGCCCCCT
  GACACTCGGGTCTATGTGGCTGACAACTGTGGCCTTTATGCCCAGGCTGAGATGTCTAATAAAGCTAGTG
  ACCATGGCTGGGTGTGTGATCAACGAGTGATGAAGTTTCACAAGTATGAGGAAGAGGAAGATGTAGAAAC
  CGGAGCCAGTCAAGATTGGCTGCCTCATGTTGAGGTTCAGAGTTACGACTCGGACTGGACAGAGGCGCCG
  GCAGCTGTGGTGATTGGCGGGGAGACCTACGGCGTGAGCCTGGAGTCCCTGCAGCTGGCCGAGAGCACTG
  GTGGCAAGAGGCTGCTGATCCCCGTTGTGCCTGGTGTGGACAGCCTCAACTCGGCCATGGCGGCAAGCAT
  CCTGCTTTTCGAAGGGAAAAGACAGCTGCGGGGGAGGGCGGAGGACTTGAGCAGGGACAGGAGTTACCAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204113 representing NM_018146
  Red=Cloning site Green=Tags(s)
  
  MAALVRPSRFVVRPLLQVVQAWDLDARRWVRALRRSPVKVVFPSGEVVEQKRAPGKQPRKAPSEASAQEQ
  REKQPLEESASRAPSTWEESGLRYDKAYPGDRRLSSVMTIVKSRPFREKQGKILLEGRRLISDALKAGAV
  PKMFFFSRLEYLKELPVDKLKGVSLIKVKFEDIKDWSDLVTPQGIMGIFAKPDHVKMTYPKTQLQHSLPL
  LLICDNLRDPGNLGTILRSAAGAGCSKVLLTKGCVDAWEPKVLRAGMGAHFRMPIINNLEWETVPNYLPP
  DTRVYVADNCGLYAQAEMSNKASDHGWVCDQRVMKFHKYEEEEDVETGASQDWLPHVEVQSYDSDWTEAP
  AAVVIGGETYGVSLESLQLAESTGGKRLLIPVVPGVDSLNSAMAASILLFEGKRQLRGRAEDLSRDRSYH
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018146
• ORF Size: 1260 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_018146.2, NP_060616.1
• RefSeq Size: 1815
• RefSeq ORF: 1263
• Locus ID: 55178
• Domains: SpoU_methylase
• Protein Families: Stem cell - Pluripotency
• Gene Summary: Efficient translation of mitochondrial-derived transcripts requires proper assembly of the large subunit of the mitochondrial ribosome. Central to the biogenesis of this large subunit is the A-loop of mitochondrial 16S rRNA, which is modified by three rRNA methyltransferases located near mtDNA nucleoids. The protein encoded by this gene methylates G(1370) of 16S rRNA, and this modification is necessary for proper ribosomal large subnit assembly. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2015]