BTN2A2 (NM_006995) Human Tagged ORF Clone

CAT#: RG204232

BTN2A2 (GFP-tagged) - Human butyrophilin, subfamily 2, member A2 (BTN2A2), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: BTN2A2
• Synonyms: BT2.2; BTF2; BTN2.2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204232 representing NM_006995
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACCAGCTGCTGCTCTGCACTTCTCCCTGCCAGCCTCCCTCCTCCTCCTCCTGCTCCTCCTCCTTC
  TCAGCCTGTGTGCACTGGTCTCAGCCCAGTTTACTGTCGTGGGGCCAGCTAATCCCATCCTGGCCATGGT
  GGGAGAAAACACTACATTACGCTGCCATCTGTCACCCGAGAAAAATGCTGAGGACATGGAGGTGCGGTGG
  TTCCGGTCTCAGTTCTCCCCCGCAGTGTTTGTGTATAAGGGTGGGAGAGAGAGAACAGAGGAGCAGATGG
  AGGAGTACCGGGGAAGAATCACCTTTGTGAGCAAAGACATCAACAGGGGCAGCGTGGCCCTGGTCATACA
  TAACGTCACAGCCCAGGAGAATGGGATCTACCGCTGTTACTTCCAAGAAGGCAGGTCCTACGATGAGGCC
  ATCCTACGCCTCGTGGTGGCAGGCCTTGGGTCTAAGCCCCTCATTGAAATCAAGGCCCAAGAGGATGGGA
  GCATCTGGCTGGAGTGCATATCTGGAGGGTGGTACCCAGAGCCCCTCACAGTGTGGAGGGACCCCTACGG
  TGAGGTTGTGCCCGCCCTGAAGGAGGTTTCCATCGCTGATGCTGACGGCCTCTTCATGGTCACCACAGCT
  GTGATCATCAGAGACAAGTATGTGAGGAATGTGTCCTGCTCTGTCAACAACACCCTGCTCGGCCAGGAGA
  AGGAAACTGTCATTTTTATTCCAGAATCCTTTATGCCCAGCGCATCTCCCTGGATGGTGGCCCTAGCTGT
  CATCCTGACCGCATCTCCCTGGATGGTGTCCATGACTGTCATCCTGGCTGTTTTCATCATCTTCATGGCT
  GTCAGCATCTGTTGCATCAAGAAACTTCAAAGGGAAAAAAAGATTCTGTCAGGGGAAAAGAAAGTTGAAC
  AAGAGGAAAAAGAAATTGCACAGCAACTTCAAGAAGAATTGCGATGGAGAAGAACATTCTTACATGCTGC
  TGATGTGGTCCTGGATCCAGACACCGCTCATCCCGAGCTCTTCCTGTCAGAGGACCGGAGAAGTGTGAGG
  CGGGGCCCCTACAGGCAGAGAGTGCCTGACAACCCAGAGAGATTCGACAGTCAGCCTTGTGTCCTGGGAT
  GGGAGAGCTTCGCCTCAGGGAAACATTACTGGGAGGTGGAGGTGGAAAACGTGATGGTGTGGACTGTGGG
  GGTCTGCAGACACAGTGTTGAGAGGAAAGGGGAGGTCCTGCTGATTCCTCAGAATGGCTTCTGGACCCTG
  GAGATGTTTGGAAACCAATACCGGGCCCTGTCCTCCCCTGAGAGGATTCTCCCTTTGAAGGAGTCCCTTT
  GCCGGGTGGGCGTCTTCCTGGACTATGAAGCTGGAGATGTCTCCTTCTACAACATGAGGGACAGATCGCA
  CATCTACACATGTCCCCGTTCAGCCTTTACTGTGCCTGTGAGGCCCTTCTTCAGGTTAGGGTCTGATGAC
  AGCCCCATCTTCATCTGCCCTGCACTCACAGGAGCCAGTGGGGTCATGGTGCCTGAAGAGGGCCTGAAAC
  TTCACAGAGTGGGGACCCACCAGAGCCTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204232 representing NM_006995
  Red=Cloning site Green=Tags(s)
  
  MEPAAALHFSLPASLLLLLLLLLLSLCALVSAQFTVVGPANPILAMVGENTTLRCHLSPEKNAEDMEVRW
  FRSQFSPAVFVYKGGRERTEEQMEEYRGRITFVSKDINRGSVALVIHNVTAQENGIYRCYFQEGRSYDEA
  ILRLVVAGLGSKPLIEIKAQEDGSIWLECISGGWYPEPLTVWRDPYGEVVPALKEVSIADADGLFMVTTA
  VIIRDKYVRNVSCSVNNTLLGQEKETVIFIPESFMPSASPWMVALAVILTASPWMVSMTVILAVFIIFMA
  VSICCIKKLQREKKILSGEKKVEQEEKEIAQQLQEELRWRRTFLHAADVVLDPDTAHPELFLSEDRRSVR
  RGPYRQRVPDNPERFDSQPCVLGWESFASGKHYWEVEVENVMVWTVGVCRHSVERKGEVLLIPQNGFWTL
  EMFGNQYRALSSPERILPLKESLCRVGVFLDYEAGDVSFYNMRDRSHIYTCPRSAFTVPVRPFFRLGSDD
  SPIFICPALTGASGVMVPEEGLKLHRVGTHQSL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006995
• ORF Size: 1569 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_006995.3, NP_008926.2
• RefSeq Size: 3577
• RefSeq ORF: 1572
• Locus ID: 10385
• Domains: IGv, IG, SPRY, PRY
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: Butyrophilin is the major protein associated with fat droplets in the milk. This gene is a member of the BTN2 subfamily of genes, which encode proteins belonging to the butyrophilin protein family. The gene is located in a cluster on chromosome 6, consisting of seven genes belonging to the expanding B7/butyrophilin-like group, a subset of the immunoglobulin gene superfamily. The encoded protein is a type I receptor glycoprotein involved in lipid, fatty-acid and sterol metabolism. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]