AdSS 2 (ADSS) (NM_001126) Human Tagged ORF Clone

CAT#: RG204256

ADSS (GFP-tagged) - Human adenylosuccinate synthase (ADSS)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ADSS
• Synonyms: ADEH; ADSS 2; ADSS2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204256 representing NM_001126
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTTCGCCGAGACCTACCCGGCGGCATCCTCCCTGCCCAACGGCGATTGCGGCCGCCCCAGGGCGC
  GGCCCGGAGGAAACCGGGTGACGGTGGTGCTCGGTGCGCAGTGGGGCGACGAAGGCAAAGGGAAGGTGGT
  GGACCTGCTGGCGCAGGACGCCGACATCGTGTGCCGCTGCCAGGGAGGAAATAATGCTGGCCATACAGTT
  GTTGTGGATTCTGTGGAATATGATTTTCATCTCTTACCCAGTGGAATAATTAATCCAAATGTCACTGCAT
  TCATTGGAAATGGTGTGGTAATTCATCTACCTGGATTGTTTGAAGAAGCAGAGAAAAATGTTCAAAAAGG
  AAAAGGACTAGAAGGCTGGGAAAAAAGGCTTATTATATCTGACAGAGCTCATATTGTATTTGATTTTCAT
  CAAGCAGCTGATGGTATCCAGGAACAACAGAGACAAGAACAAGCAGGAAAAAATTTGGGTACAACAAAAA
  AGGGCATTGGCCCAGTTTATTCGTCCAAAGCTGCTCGGAGTGGACTCAGGATGTGCGACCTTGTTTCTGA
  CTTTGATGGCTTCTCTGAGAGGTTTAAAGTTCTAGCTAACCAATACAAATCTATATACCCCACTTTGGAA
  ATAGACATTGAAGGTGAATTACAAAAACTCAAGGGTTATATGGAAAAGATTAAACCAATGGTGAGAGATG
  GAGTTTATTTTCTATATGAGGCCCTACATGGACCACCAAAGAAAATCTTGGTAGAAGGTGCAAATGCAGC
  ACTATTAGATATTGATTTTGGGACTTACCCTTTTGTAACCTCTTCAAATTGTACTGTTGGAGGTGTTTGT
  ACTGGTTTGGGTATGCCACCTCAAAATGTTGGAGAAGTGTATGGAGTTGTGAAAGCTTATACAACTAGAG
  TTGGTATTGGTGCCTTTCCTACAGAGCAAGACAATGAAATTGGAGAATTATTACAAACAAGGGGTAGAGA
  GTTTGGTGTAACTACTGGAAGGAAAAGAAGATGTGGCTGGTTGGACCTCGTTTTGCTCAAATATGCTCAT
  ATGATCAATGGATTTACTGCGTTGGCACTTACCAAGTTGGATATTTTGGACATGTTTACGGAAATCAAAG
  TTGGAGTTGCTTACAAGTTAGATGGTGAAATCATACCTCATATCCCAGCAAACCAAGAAGTCTTAAATAA
  AGTTGAAGTTCAATATAAGACTCTCCCAGGATGGAACACAGACATATCAAATGCAAGGGCGTTTAAAGAA
  CTACCTGTTAATGCACAAAACTATGTTCGATTTATTGAAGATGAGCTTCAAATTCCAGTTAAGTGGATTG
  GTGTTGGTAAATCCAGAGAATCTATGATTCAACTCTTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204256 representing NM_001126
  Red=Cloning site Green=Tags(s)
  
  MAFAETYPAASSLPNGDCGRPRARPGGNRVTVVLGAQWGDEGKGKVVDLLAQDADIVCRCQGGNNAGHTV
  VVDSVEYDFHLLPSGIINPNVTAFIGNGVVIHLPGLFEEAEKNVQKGKGLEGWEKRLIISDRAHIVFDFH
  QAADGIQEQQRQEQAGKNLGTTKKGIGPVYSSKAARSGLRMCDLVSDFDGFSERFKVLANQYKSIYPTLE
  IDIEGELQKLKGYMEKIKPMVRDGVYFLYEALHGPPKKILVEGANAALLDIDFGTYPFVTSSNCTVGGVC
  TGLGMPPQNVGEVYGVVKAYTTRVGIGAFPTEQDNEIGELLQTRGREFGVTTGRKRRCGWLDLVLLKYAH
  MINGFTALALTKLDILDMFTEIKVGVAYKLDGEIIPHIPANQEVLNKVEVQYKTLPGWNTDISNARAFKE
  LPVNAQNYVRFIEDELQIPVKWIGVGKSRESMIQLF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001126
• ORF Size: 1368 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001126.2, NP_001117.2
• RefSeq Size: 2775 bp
• RefSeq ORF: 1371 bp
• Locus ID: 159
• Cytogenetics: 1q44
• Domains: Adenylsucc_synt
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Metabolic pathways, Purine metabolism
• Gene Summary: 'This gene encodes the enzyme adenylosuccinate synthetase which catalyzes the first committed step in the conversion of inosine monophosphate to adenosine monophosphate. A pseudogene of this gene is found on chromosome 17.[provided by RefSeq, Nov 2010]'