MIR16 (GDE1) (NM_016641) Human Tagged ORF Clone

CAT#: RG204923

GDE1 (GFP-tagged) - Human glycerophosphodiester phosphodiesterase 1 (GDE1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GDE1
• Synonyms: 363E6.2; MIR16
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204923 representing NM_016641
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCTGTGGGAGGACCAGGGCGGCCTCCTGGGCCCTTTCTCCTTCCTGCTGCTAGTGCTGCTGCTGG
  TGACGCGGAGCCCGGTCAATGCCTGCCTCCTCACCGGCAGCCTCTTCGTTCTACTGCGCGTCTTCAGCTT
  TGAGCCGGTGCCCTCTTGCAGGGCCCTGCAGGTGCTCAAGCCCCGGGACCGCATTTCTGCCATCGCCCAC
  CGTGGCGGCAGCCACGACGCGCCCGAGAACACGCTGGCGGCCATTCGGCAGGCAGCTAAGAATGGAGCAA
  CAGGCGTGGAGTTGGACATTGAGTTTACTTCTGACGGGATTCCTGTCTTAATGCACGATAACACAGTAGA
  TAGGACGACTGATGGGACTGGGCGATTGTGTGATTTGACATTTGAACAAATTAGGAAGCTGAATCCTGCA
  GCAAACCACAGACTCAGGAATGATTTCCCTGATGAAAAGATCCCTACCCTAAGGGAAGCTGTTGCAGAGT
  GCCTAAACCATAACCTCACAATCTTCTTTGATGTCAAAGGCCATGCACACAAGGCTACTGAGGCTCTAAA
  GAAAATGTATATGGAATTTCCTCAACTGTATAATAATAGTGTGGTCTGTTCTTTCTTGCCAGAAGTTATC
  TACAAGATGAGACAAACAGATCGGGATGTAATAACAGCATTAACTCACAGACCTTGGAGCCTAAGCCATA
  CAGGAGATGGGAAACCACGCTATGATACTTTCTGGAAACATTTTATATTTGTTATGATGGACATTTTGCT
  CGATTGGAGCATGCATAATATCTTGTGGTACCTGTGTGGAATTTCAGCTTTCCTCATGCAAAAGGATTTT
  GTATCCCCGGCCTACTTGAAGAAGTGGTCAGCTAAAGGAATCCAGGTTGTTGGTTGGACTGTTAATACCT
  TTGATGAAAAGAGTTACTACGAATCCCATCTTGGTTCCAGCTATATCACTGACAGCATGGTAGAAGACTG
  CGAACCTCACTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204923 representing NM_016641
  Red=Cloning site Green=Tags(s)
  
  MWLWEDQGGLLGPFSFLLLVLLLVTRSPVNACLLTGSLFVLLRVFSFEPVPSCRALQVLKPRDRISAIAH
  RGGSHDAPENTLAAIRQAAKNGATGVELDIEFTSDGIPVLMHDNTVDRTTDGTGRLCDLTFEQIRKLNPA
  ANHRLRNDFPDEKIPTLREAVAECLNHNLTIFFDVKGHAHKATEALKKMYMEFPQLYNNSVVCSFLPEVI
  YKMRQTDRDVITALTHRPWSLSHTGDGKPRYDTFWKHFIFVMMDILLDWSMHNILWYLCGISAFLMQKDF
  VSPAYLKKWSAKGIQVVGWTVNTFDEKSYYESHLGSSYITDSMVEDCEPHF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016641
• ORF Size: 993 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_016641.3, NP_057725.1
• RefSeq Size: 2960
• RefSeq ORF: 996
• Locus ID: 51573
• Domains: GDPD
• Protein Families: Transmembrane
• Protein Pathways: Glycerophospholipid metabolism