GPRC5B (NM_016235) Human Tagged ORF Clone

CAT#: RG205201

GPRC5B (GFP-tagged) - Human G protein-coupled receptor, family C, group 5, member B (GPRC5B)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GPRC5B
• Synonyms: RAIG-2; RAIG2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205201 representing NM_016235
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCGTGGCATCAGAGAGAAAGATGAGAGCTCACCAGGTGCTCACCTTCCTCCTGCTCTTCGTGATCA
  CCTCGGTGGCCTCTGAAAACGCCAGCACATCCCGAGGCTGTGGGCTGGACCTCCTCCCTCAGTACGTGTC
  CCTGTGCGACCTGGACGCCATCTGGGGCATTGTGGTGGAGGCGGTGGCCGGGGCGGGCGCCCTGATCACA
  CTGCTCCTGATGCTCATCCTCCTGGTGCGGCTGCCCTTCATCAAGGAGAAGGAGAAGAAGAGCCCTGTGG
  GCCTCCACTTTCTGTTCCTCCTGGGGACCCTGGGCCTCTTTGGGCTGACGTTTGCCTTCATCATCCAGGA
  GGACGAGACCATCTGCTCTGTCCGCCGCTTCCTCTGGGGCGTCCTCTTTGCGCTCTGCTTCTCCTGCCTG
  CTGAGCCAGGCATGGCGCGTGCGGAGGCTGGTGCGGCATGGCACGGGCCCCGCGGGCTGGCAGCTGGTGG
  GCCTGGCGCTGTGCCTGATGCTGGTGCAAGTCATCATCGCTGTGGAGTGGCTGGTGCTCACCGTGCTGCG
  TGACACAAGGCCAGCCTGCGCCTACGAGCCCATGGACTTTGTGATGGCCCTCATCTACGACATGGTACTG
  CTTGTGGTCACCCTGGGGCTGGCCCTCTTCACTCTGTGCGGCAAGTTCAAGAGGTGGAAGCTGAACGGGG
  CCTTCCTCCTCATCACAGCCTTCCTCTCTGTGCTCATCTGGGTGGCCTGGATGACCATGTACCTCTTCGG
  CAATGTCAAGCTGCAGCAGGGGGATGCCTGGAACGACCCCACCTTGGCCATCACGCTGGCGGCCAGCGGC
  TGGGTCTTCGTCATCTTCCACGCCATCCCTGAGATCCACTGCACCCTTCTGCCAGCCCTGCAGGAGAACA
  CGCCCAACTACTTCGACACGTCGCAGCCCAGGATGCGGGAGACGGCCTTCGAGGAGGACGTGCAGCTGCC
  GCGGGCCTATATGGAGAACAAGGCCTTCTCCATGGATGAACACAATGCAGCTCTCCGAACAGCAGGATTT
  CCCAACGGCAGCTTGGGAAAAAGACCCAGTGGCAGCTTGGGGAAAAGACCCAGCGCTCCGTTTAGAAGCA
  ACGTGTATCAGCCAACTGAGATGGCCGTCGTGCTCAACGGTGGGACCATCCCAACTGCTCCGCCAAGTCA
  CACAGGAAGACACCTTTGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205201 representing NM_016235
  Red=Cloning site Green=Tags(s)
  
  MFVASERKMRAHQVLTFLLLFVITSVASENASTSRGCGLDLLPQYVSLCDLDAIWGIVVEAVAGAGALIT
  LLLMLILLVRLPFIKEKEKKSPVGLHFLFLLGTLGLFGLTFAFIIQEDETICSVRRFLWGVLFALCFSCL
  LSQAWRVRRLVRHGTGPAGWQLVGLALCLMLVQVIIAVEWLVLTVLRDTRPACAYEPMDFVMALIYDMVL
  LVVTLGLALFTLCGKFKRWKLNGAFLLITAFLSVLIWVAWMTMYLFGNVKLQQGDAWNDPTLAITLAASG
  WVFVIFHAIPEIHCTLLPALQENTPNYFDTSQPRMRETAFEEDVQLPRAYMENKAFSMDEHNAALRTAGF
  PNGSLGKRPSGSLGKRPSAPFRSNVYQPTEMAVVLNGGTIPTAPPSHTGRHLW
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016235
• ORF Size: 1209 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_016235.1, NP_057319.1
• RefSeq Size: 2870
• RefSeq ORF: 1212
• Locus ID: 51704
• Domains: 7tm_3
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a member of the type 3 G protein-coupled receptor family. Members of this superfamily are characterized by a signature 7-transmembrane domain motif. The encoded protein may modulate insulin secretion and increased protein expression is associated with type 2 diabetes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2015]