TIF1 alpha (TRIM24) (NM_015905) Human Tagged ORF Clone

CAT#: RG205332

TRIM24 (GFP-tagged) - Human tripartite motif containing 24 (TRIM24), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TRIM24
• Synonyms: hTIF1; PTC6; RNF82; TF1A; TIF1; TIF1A; TIF1ALPHA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205332 representing NM_015905
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGTGGCGGTGGAGAAGGCGGTGGCGGCGGCGGCAGCGGCCTCGGCTGCGGCCTCCGGGGGGCCCT
  CGGCGGCGCCGAGCGGGGAGAACGAGGCCGAGAGTCGGCAGGGCCCGGACTCGGAGCGCGGCGGCGAGGC
  GGCCCGGCTCAACCTGTTGGACACTTGCGCCGTGTGCCACCAGAACATCCAGAGCCGGGCGCCCAAGCTG
  CTGCCCTGCCTGCACTCTTTCTGCCAGCGCTGCCTGCCCGCGCCCCAGCGCTACCTCATGCTGCCCGCGC
  CCATGCTGGGCTCGGCCGAGACCCCGCCACCCGTCCCTGCCCCCGGCTCGCCGGTCAGCGGCTCGTCGCC
  GTTCGCCACCCAAGTTGGAGTCATTCGTTGCCCAGTTTGCAGCCAAGAATGTGCAGAGAGACACATCATA
  GATAACTTTTTTGTGAAGGACACTACTGAGGTTCCCAGCAGTACAGTAGAAAAGTCAAATCAGGTATGTA
  CAAGCTGTGAGGACAACGCAGAAGCCAATGGGTTTTGTGTAGAGTGTGTTGAATGGCTCTGCAAGACGTG
  TATCAGAGCTCATCAGAGGGTAAAGTTCACAAAAGACCACACTGTCAGACAGAAAGAGGAAGTATCTCCA
  GAGGCAGTTGGTGTCACCAGCCAGCGACCAGTGTTTTGTCCTTTTCATAAAAAGGAGCAGCTGAAGCTGT
  ACTGTGAGACATGTGACAAACTGACATGTCGAGACTGTCAGTTGTTAGAACATAAAGAGCATAGATACCA
  ATTTATAGAAGAAGCTTTTCAGAATCAGAAAGTGATCATAGATACACTAATCACCAAACTGATGGAAAAA
  ACAAAATACATAAAATTCACAGGAAATCAGATCCAAAACAGAATTATTGAAGTAAATCAAAATCAAAAGC
  AGGTGGAACAGGATATTAAAGTTGCTATATTTACACTGATGGTAGAAATAAATAAAAAAGGAAAAGCTCT
  ACTGCATCAGTTAGAGAGCCTTGCAAAGGACCATCGCATGAAACTTATGCAACAACAACAGGAAGTGGCT
  GGACTCTCTAAACAATTGGAGCATGTCATGCATTTTTCTAAATGGGCAGTTTCCAGTGGCAGCAGTACAG
  CATTACTTTATAGCAAACGACTGATTACATACCGGTTACGACACCTCCTTCGTGCAAGGTGTGATGCATC
  CCCAGTGACCAACAACACCATCCAATTTCACTGTGATCCTAGTTTCTGGGCTCAAAATATCATCAACTTA
  GGTTCTTTAGTAATCGAGGATAAAGAGAGCCAGCCACAAATGCCTAAGCAGAATCCTGTCGTGGAACAGA
  ATTCACAGCCACCAAGTGGTTTATCATCAAACCAGTTATCCAAGTTCCCAACACAGATCAGCCTAGCTCA
  ATTACGGCTCCAGCATATGCAGCAACAGGTAATGGCTCAGAGGCAACAGGTGCAACGGAGGCCAGCACCT
  GTGGGTTTACCAAACCCTAGAATGCAGGGGCCCATCCAGCAACCTTCCATCTCTCATCAGCAACCGCCTC
  CACGTTTGATAAACTTTCAGAATCACAGCCCCAAACCCAATGGACCAGTTCTTCCTCCTCATCCTCAACA
  ACTGAGATATCCACCAAACCAGAACATACCACGACAAGCAATAAAGCCAAACCCCCTACAGATGGCTTTC
  TTGGCTCAACAAGCCATAAAACAGTGGCAGATCAGCAGTGGACAGGGAACCCCATCAACTACCAACAGCA
  CATCCTCTACTCCTTCCAGCCCCACGATTACTAGTGCAGCAGGATATGATGGAAAGGCTTTTGGTTCACC
  TATGATCGATTTGAGCTCACCAGTGGGAGGGTCTTATAATCTTCCCTCTCTTCCGGATATTGACTGTTCA
  AGTACTATTATGCTGGACAATATTGTGAGGAAAGATACTAATATAGATCATGGCCAGCCAAGACCACCCT
  CAAACAGAACGGTCCAGTCACCAAATTCATCAGTGCCATCTCCAGGCCTTGCAGGACCTGTTACTATGAC
  TAGTGTACACCCCCCAATACGTTCACCTAGTGCCTCCAGCGTTGGAAGCCGAGGAAGCTCTGGCTCTTCC
  AGCAAACCAGCAGGAGCTGACTCTACACACAAAGTCCCAGTGGTCATGCTGGAGCCAATTCGAATAAAAC
  AAGAAAACAGTGGACCACCGGAAAATTATGATTTCCCTGTTGTTATAGTGAAGCAAGAATCAGATGAAGA
  ATCTAGGCCTCAAAATGCCAATTATCCAAGAAGCATACTCACCTCCCTGCTCTTAAATAGCAGTCAGAGC
  TCTACTTCTGAGGAGACTGTGCTAAGATCAGATGCCCCTGATAGTACAGGAGATCAACCTGGACTTCACC
  AGGACAATTCCTCAAATGGAAAGTCTGAATGGTTGGATCCTTCCCAGAAGTCACCTCTTCATGTTGGAGA
  GACAAGGAAAGAGGATGACCCCAATGAGGACTGGTGTGCAGTTTGTCAAAACGGAGGGGAACTCCTCTGC
  TGTGAAAAGTGCCCCAAAGTATTCCATCTTTCTTGTCATGTGCCCACATTGACAAATTTTCCAAGTGGAG
  AGTGGATTTGCACTTTCTGCCGAGACTTATCTAAACCAGAAGTTGAATATGATTGTGATGCTCCCAGTCA
  CAACTCAGAAAAAAAGAAAACTGAAGGCCTTGTTAAGTTAACACCTATAGATAAAAGGAAGTGTGAGCGC
  CTACTTTTATTTCTTTACTGCCATGAAATGAGCCTGGCTTTTCAAGACCCTGTTCCTCTAACTGTGCCTG
  ATTATTACAAAATAATTAAAAATCCAATGGATTTGTCAACCATCAAGAAAAGACTACAAGAAGATTATTC
  CATGTACTCAAAACCTGAAGATTTTGTAGCTGATTTTAGATTGATCTTTCAAAACTGTGCTGAATTCAAT
  GAGCCTGATTCAGAAGTAGCCAATGCTGGTATAAAACTTGAAAATTATTTTGAAGAACTTCTAAAGAACC
  TCTATCCAGAAAAAAGGTTTCCCAAACCAGAATTCAGGAATGAATCAGAAGATAATAAATTTAGTGATGA
  TTCAGATGATGACTTTGTACAGCCCCGGAAGAAACGCCTCAAAAGCATTGAAGAACGCCAGTTGCTTAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205332 representing NM_015905
  Red=Cloning site Green=Tags(s)
  
  MEVAVEKAVAAAAAASAAASGGPSAAPSGENEAESRQGPDSERGGEAARLNLLDTCAVCHQNIQSRAPKL
  LPCLHSFCQRCLPAPQRYLMLPAPMLGSAETPPPVPAPGSPVSGSSPFATQVGVIRCPVCSQECAERHII
  DNFFVKDTTEVPSSTVEKSNQVCTSCEDNAEANGFCVECVEWLCKTCIRAHQRVKFTKDHTVRQKEEVSP
  EAVGVTSQRPVFCPFHKKEQLKLYCETCDKLTCRDCQLLEHKEHRYQFIEEAFQNQKVIIDTLITKLMEK
  TKYIKFTGNQIQNRIIEVNQNQKQVEQDIKVAIFTLMVEINKKGKALLHQLESLAKDHRMKLMQQQQEVA
  GLSKQLEHVMHFSKWAVSSGSSTALLYSKRLITYRLRHLLRARCDASPVTNNTIQFHCDPSFWAQNIINL
  GSLVIEDKESQPQMPKQNPVVEQNSQPPSGLSSNQLSKFPTQISLAQLRLQHMQQQVMAQRQQVQRRPAP
  VGLPNPRMQGPIQQPSISHQQPPPRLINFQNHSPKPNGPVLPPHPQQLRYPPNQNIPRQAIKPNPLQMAF
  LAQQAIKQWQISSGQGTPSTTNSTSSTPSSPTITSAAGYDGKAFGSPMIDLSSPVGGSYNLPSLPDIDCS
  STIMLDNIVRKDTNIDHGQPRPPSNRTVQSPNSSVPSPGLAGPVTMTSVHPPIRSPSASSVGSRGSSGSS
  SKPAGADSTHKVPVVMLEPIRIKQENSGPPENYDFPVVIVKQESDEESRPQNANYPRSILTSLLLNSSQS
  STSEETVLRSDAPDSTGDQPGLHQDNSSNGKSEWLDPSQKSPLHVGETRKEDDPNEDWCAVCQNGGELLC
  CEKCPKVFHLSCHVPTLTNFPSGEWICTFCRDLSKPEVEYDCDAPSHNSEKKKTEGLVKLTPIDKRKCER
  LLLFLYCHEMSLAFQDPVPLTVPDYYKIIKNPMDLSTIKKRLQEDYSMYSKPEDFVADFRLIFQNCAEFN
  EPDSEVANAGIKLENYFEELLKNLYPEKRFPKPEFRNESEDNKFSDDSDDDFVQPRKKRLKSIEERQLLK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015905
• ORF Size: 3150 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015905.2, NP_056989.2
• RefSeq Size: 4007
• RefSeq ORF: 3153
• Locus ID: 8805
• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors
• Gene Summary: The protein encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]