PODN (NM_153703) Human Tagged ORF Clone

CAT#: RG205567

PODN (GFP-tagged) - Human podocan (PODN), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PODN
• Synonyms: PCAN; SLRR5A
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205567 representing NM_153703
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGGAGCCCGAGCCCGCGGAGCGCAGCTGAGACTGGGGGAGCGCGTTCGGCCTGTGGGGCGCCGCT
  CGGCGCCGGGGCGCAGCAGGTTCCATCAGCCCTGGCGCCCAGGCGCATCTGACTCGGCACCCCCTGCAGG
  CACCATGGCCCAGAGCCGGGTGCTGCTGCTCCTGCTGCTGCTGCCGCCACAGCTGCACCTGGGACCTGTG
  CTTGCCGTGAGGGCCCCAGGATTTGGCCGAAGTGGCGGCCACAGCCTGAGCCCCGAAGAGAACGAATTTG
  CGGAGGAGGAGCCGGTGCTGGTACTGAGCCCTGAGGAGCCCGGGCCTGGCCCAGCCGCGGTCAGCTGCCC
  CCGAGACTGTGCCTGTTCCCAGGAGGGCGTCGTGGACTGTGGCGGTATTGACCTGCGTGAGTTCCCGGGG
  GACCTGCCTGAGCACACCAACCACCTATCTCTGCAGAACAACCAGCTGGAAAAGATCTACCCTGAGGAGC
  TCTCCCGGCTGCACCGGCTGGAGACACTGAACCTGCAAAACAACCGCCTGACTTCCCGAGGGCTCCCAGA
  GAAGGCGTTTGAGCATCTGACCAACCTCAATTACCTGTACTTGGCCAATAACAAGCTGACCTTGGCACCC
  CGCTTCCTGCCAAACGCCCTGATCAGTGTGGACTTTGCTGCCAACTATCTCACCAAGATCTATGGGCTCA
  CCTTTGGCCAGAAGCCAAACTTGAGGTCTGTGTACCTGCACAACAACAAGCTGGCAGACGCCGGGCTGCC
  GGACAACATGTTCAACGGCTCCAGCAACGTCGAGGTCCTCATCCTGTCCAGCAACTTCCTGCGCCACGTG
  CCCAAGCACCTGCCGCCTGCCCTGTACAAGCTGCACCTCAAGAACAACAAGCTGGAGAAGATCCCCCCGG
  GGGCCTTCAGCGAGCTGAGCAGCCTGCGCGAGCTATACCTGCAGAACAACTACCTGACTGACGAGGGCCT
  GGACAACGAGACCTTCTGGAAGCTCTCCAGCCTGGAGTACCTGGATCTGTCCAGCAACAACCTGTCTCGG
  GTCCCAGCTGGGCTGCCGCGCAGCCTGGTGCTGCTGCACTTGGAGAAGAACGCCATCCGGAGCGTGGACG
  CGAATGTGCTGACCCCCATCCGCAGCCTGGAGTACCTGCTGCTGCACAGCAACCAGCTGCGGGAGCAGGG
  CATCCACCCACTGGCCTTCCAGGGCCTCAAGCGGTTGCACACGGTGCACCTGTACAACAACGCGCTGGAG
  CGCGTGCCCAGTGGCCTGCCTCGCCGCGTGCGCACCCTCATGATCCTGCACAACCAGATCACAGGCATTG
  GCCGCGAAGACTTTGCCACCACCTACTTCCTGGAGGAGCTCAACCTCAGCTACAACCGCATCACCAGCCC
  ACAGGTGCACCGCGACGCCTTCCGCAAGCTGCGCCTGCTGCGCTCGCTGGACCTGTCGGGCAACCGGCTG
  CACACGCTGCCACCTGGGCTGCCTCGAAATGTCCATGTGCTGAAGGTCAAGCGCAATGAGCTGGCTGCCT
  TGGCACGAGGGGCGCTGGCGGGCATGGCTCAGCTGCGTGAGCTGTACCTCACCAGCAACCGACTGCGCAG
  CCGAGCCCTGGGCCCCCGTGCCTGGGTGGACCTCGCCCATCTGCAGCTGCTGGACATCGCCGGGAATCAG
  CTCACAGAGATCCCCGAGGGGCTCCCCGAGTCACTTGAGTACCTGTACCTGCAGAACAACAAGATTAGTG
  CGGTGCCCGCCAATGCCTTCGACTCCACGCCCAACCTCAAGGGGATCTTTCTCAGGTTTAACAAGCTGGC
  TGTGGGCTCCGTGGTGGACAGTGCCTTCCGGAGGCTGAAGCACCTGCAGGTCTTGGACATTGAAGGCAAC
  TTAGAGTTTGGTGACATTTCCAAGGACCGTGGCCGCTTGGGGAAGGAAAAGGAGGAGGAGGAAGAGGAGG
  AGGAGGAGGAAGAGGAAACAAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205567 representing NM_153703
  Red=Cloning site Green=Tags(s)
  
  MEGARARGAQLRLGERVRPVGRRSAPGRSRFHQPWRPGASDSAPPAGTMAQSRVLLLLLLLPPQLHLGPV
  LAVRAPGFGRSGGHSLSPEENEFAEEEPVLVLSPEEPGPGPAAVSCPRDCACSQEGVVDCGGIDLREFPG
  DLPEHTNHLSLQNNQLEKIYPEELSRLHRLETLNLQNNRLTSRGLPEKAFEHLTNLNYLYLANNKLTLAP
  RFLPNALISVDFAANYLTKIYGLTFGQKPNLRSVYLHNNKLADAGLPDNMFNGSSNVEVLILSSNFLRHV
  PKHLPPALYKLHLKNNKLEKIPPGAFSELSSLRELYLQNNYLTDEGLDNETFWKLSSLEYLDLSSNNLSR
  VPAGLPRSLVLLHLEKNAIRSVDANVLTPIRSLEYLLLHSNQLREQGIHPLAFQGLKRLHTVHLYNNALE
  RVPSGLPRRVRTLMILHNQITGIGREDFATTYFLEELNLSYNRITSPQVHRDAFRKLRLLRSLDLSGNRL
  HTLPPGLPRNVHVLKVKRNELAALARGALAGMAQLRELYLTSNRLRSRALGPRAWVDLAHLQLLDIAGNQ
  LTEIPEGLPESLEYLYLQNNKISAVPANAFDSTPNLKGIFLRFNKLAVGSVVDSAFRRLKHLQVLDIEGN
  LEFGDISKDRGRLGKEKEEEEEEEEEEEETR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153703
• ORF Size: 1983 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_153703.3, NP_714914.2
• RefSeq Size: 3019
• RefSeq ORF: 1986
• Locus ID: 127435
• Domains: LRR, LRR_TYP, LRR_BAC, LRR_PS
• Protein Families: Secreted Protein
• Gene Summary: The protein encoded by this gene is a member of the small leucine-rich repeat protein family and contains an amino terminal CX3CXCX7C cysteine-rich cluster followed by a leucine-rich repeat domain. Studies suggest that this protein could function to inhibit smooth muscle cell proliferation and migration following arterial injury. [provided by RefSeq, Jul 2016]