PORCN (NM_203473) Human Tagged ORF Clone

CAT#: RG205717

PORCN (GFP-tagged) - Human porcupine homolog (Drosophila) (PORCN), transcript variant B

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PORCN
• Synonyms: DHOF; FODH; MG61; PORC; PPN
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205717 representing NM_203473
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACCTTTAGCCGCCAGGAATTTTTCCAGCAGCTACTGCAAGGCTGTCTCCTGCCTACTGCCCAGC
  AGGGCCTTGACCAGATCTGGCTGCTCCTTGCCATCTGCCTCGCCTGCCGCCTCCTCTGGAGGCTCGGGTT
  GCCATCCTACCTGAAGCATGCAAGCACCGTGGCAGGCGGGTTCTTCAGCCTCTACCACTTCTTCCAGCTG
  CACATGGTTTGGGTCGTGCTGCTCAGCCTCCTGTGCTACCTCGTGCTGTTCCTCTGCCGACATTCCTCCC
  ATCGAGGCGTCTTCCTATCCGTCACCATCCTCATCTACCTACTCATGGGTGAGATGCACATGGTAGACAC
  CGTGACATGGCACAAGATGCGAGGGGCACAGATGATTGTGGCCATGAAGGCAGTGTCTCTGGGCTTCGAC
  CTGGACCGGGGCGAGGTGGGTACGGTGCCCTCGCCAGTGGAGTTCATGGGCTACCTCTACTTCGTGGGCA
  CCATCGTCTTCGGGCCCTGGATATCCTTCCACAGCTACCTACAAGCTGTCCAAGGCCGCCCACTGAGCTG
  CCGGTGGCTGCAGAAGGTGGCCCGGAGCCTGGCACTGGCCCTGCTGTGCCTTGTGCTGTCCACTTGCGTG
  GGCCCCTACCTCTTCCCGTACTTCATCCCCCTCAACGGTGACCGCCTCCTTCGCAACAAGAAACGCAAAG
  CCAGGTGGCTGCGAGCCTACGAGAGTGCTGTCTCCTTCCACTTCAGCAACTATTTTGTGGGCTTTCTTTC
  CGAGGCCACGGCCACGTTGGCGGGGGCTGGCTTTACCGAGGAGAAGGATCACCTGGAATGGGACCTGACG
  GTGTCCAAGCCACTGAATGTGGAGCTGCCTCGGTCAATGGTGGAAGTTGTCACAAGCTGGAACCTGCCCA
  TGTCTTATTGGCTAAATAACTATGTTTTCAAGAATGCTCTCCGCCTGGGGACCTTCTCGGCTGTGCTGGT
  CACCTATGCAGCCAGCGCCCTCCTACATGGCTTCAGTTTCCACCTGGCTGCGGTCCTGCTGTCCCTGGCT
  TTTATCACTTACGTGGAGCATGTCCTCCGGAAGCGCCTGGCTCGGATCCTCAGTGCCTGTGTCTTGTCAA
  AGCGGTGCCCGCCAGACTGTTCGCACCAGCATCGCTTGGGCCTGGGGGTGCGAGCCTTAAACTTGCTCTT
  TGGAGCTCTGGCCATCTTCCACCTGGCCTACCTGGGCTCCCTGTTTGATGTCGATGTGGATGACACCACA
  GAGGAGCAGGGCTACGGCATGGCATACACTGTCCACAAGTGGTCAGAGCTCAGCTGGGCCAGTCACTGGG
  TCACTTTTGGATGCTGGATCTTCTACCGTCTCATAGGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205717 representing NM_203473
  Red=Cloning site Green=Tags(s)
  
  MATFSRQEFFQQLLQGCLLPTAQQGLDQIWLLLAICLACRLLWRLGLPSYLKHASTVAGGFFSLYHFFQL
  HMVWVVLLSLLCYLVLFLCRHSSHRGVFLSVTILIYLLMGEMHMVDTVTWHKMRGAQMIVAMKAVSLGFD
  LDRGEVGTVPSPVEFMGYLYFVGTIVFGPWISFHSYLQAVQGRPLSCRWLQKVARSLALALLCLVLSTCV
  GPYLFPYFIPLNGDRLLRNKKRKARWLRAYESAVSFHFSNYFVGFLSEATATLAGAGFTEEKDHLEWDLT
  VSKPLNVELPRSMVEVVTSWNLPMSYWLNNYVFKNALRLGTFSAVLVTYAASALLHGFSFHLAAVLLSLA
  FITYVEHVLRKRLARILSACVLSKRCPPDCSHQHRLGLGVRALNLLFGALAIFHLAYLGSLFDVDVDDTT
  EEQGYGMAYTVHKWSELSWASHWVTFGCWIFYRLIG
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_203473
• ORF Size: 1368 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_203473.1, NP_982299.1
• RefSeq Size: 1867
• RefSeq ORF: 1371
• Locus ID: 64840
• Protein Families: Transmembrane
• Protein Pathways: Wnt signaling pathway
• Gene Summary: This gene belongs to the evolutionarily conserved porcupine (Porc) gene family. Genes of the porcupine family encode endoplasmic reticulum proteins with multiple transmembrane domains. Porcupine proteins are involved in the processing of Wnt (wingless and int homologue) proteins. Disruption of this gene is associated with focal dermal hypoplasia, and the encoded protein has been implicated in cancer. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Aug 2013]