MTAP (NM_002451) Human Tagged ORF Clone

CAT#: RG206024

MTAP (GFP-tagged) - Human methylthioadenosine phosphorylase (MTAP)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MTAP
• Synonyms: BDMF; c86fus; DMSFH; DMSMFH; HEL-249; LGMBF; MSAP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG206024 representing NM_002451
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCTGGCACCACCACCACCGCCGTGAAGATTGGAATAATTGGTGGAACAGGCCTGGATGATCCAG
  AAATTTTAGAAGGAAGAACTGAAAAATATGTGGATACTCCATTTGGCAAGCCATCTGATGCCTTAATTTT
  GGGGAAGATAAAAAATGTTGATTGCATCCTCCTTGCAAGGCATGGAAGGCAGCACACCATCATGCCTTCA
  AAGGTCAACTACCAGGCGAACATCTGGGCTTTGAAGGAAGAGGGCTGTACACATGTCATAGTGACCACAG
  CTTGTGGCTCCTTGAGGGAGGAGATTCAGCCCGGCGATATTGTCATTATTGATCAGTTCATTGACAGGAC
  CACTATGAGACCTCAGTCCTTCTATGATGGAAGTCATTCTTGTGCCAGAGGAGTGTGCCATATTCCAATG
  GCTGAGCCGTTTTGCCCAAAAACGAGAGAGGTTCTTATAGAGACTGCTAAGAAGCTAGGACTCCGGTGCC
  ACTCAAAGGGGACAATGGTCACAATCGAGGGACCTCGTTTTAGCTCCCGGGCAGAAAGCTTCATGTTCCG
  TACCTGGGGGGCGGATGTTATCAACATGACCACAGTTCCAGAGGTGGTTCTTGCTAAGGAGGCTGGAATT
  TGTTACGCAAGTATCGCCATGGCGACAGATTATGACTGCTGGAAGGAGCACGAGGAAGCAGTTTCGGTGG
  ACCGGGTCTTAAAGACCCTGAAAGAAAACGCTAATAAAGCCAAAAGCTTACTGCTCACTACCATACCTCA
  GATAGGGTCCACAGAATGGTCAGAAACCCTCCATAACCTGAAGAATATGGCCCAGTTTTCTGTTTTATTA
  CCAAGACAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG206024 representing NM_002451
  Red=Cloning site Green=Tags(s)
  
  MASGTTTTAVKIGIIGGTGLDDPEILEGRTEKYVDTPFGKPSDALILGKIKNVDCILLARHGRQHTIMPS
  KVNYQANIWALKEEGCTHVIVTTACGSLREEIQPGDIVIIDQFIDRTTMRPQSFYDGSHSCARGVCHIPM
  AEPFCPKTREVLIETAKKLGLRCHSKGTMVTIEGPRFSSRAESFMFRTWGADVINMTTVPEVVLAKEAGI
  CYASIAMATDYDCWKEHEEAVSVDRVLKTLKENANKAKSLLLTTIPQIGSTEWSETLHNLKNMAQFSVLL
  PRH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002451
• ORF Size: 849 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002451.3, NP_002442.2
• RefSeq Size: 4937 bp
• RefSeq ORF: 852 bp
• Locus ID: 4507
• Cytogenetics: 9p21.3
• Domains: Mtap_PNP
• Protein Families: Druggable Genome
• Protein Pathways: Cysteine and methionine metabolism, Metabolic pathways
• Gene Summary: 'This gene encodes an enzyme that plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. The encoded enzyme is deficient in many cancers because this gene and the tumor suppressor p16 gene are co-deleted. Multiple alternatively spliced transcript variants have been described for this gene, but their full-length natures remain unknown. [provided by RefSeq, Jul 2008]'